Optimization of sodium hydroxide pretreatment and enzyme loading for efficient hydrolysis of rice straw to improve succinate production by metabolically engineered Escherichia coli KJ122 under simultaneous saccharification and fermentation

Sawisit, Apichai; Jampatesh, Surawee; Jantama, Sirima Suvarnakuta; Jantama, Kaemwich

doi:10.1016/j.biortech.2018.03.107

Cited by 22 publications

(7 citation statements)

References 40 publications

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“…Comparatively, the addition of EPH caused a 13.16% increment of delignification. Compared with these publications which pretreat straw and grass with NaOH, this biphasic EPH system showed a competitive delignification and enzymatic digestibility of residue under more moderate and less water-consuming conditions. ,, Although weak-alkali catalysts like n -propylamine, N 2 CO 3 , and ammonia can result in similar delignification and enzymatic digestibility, the experimental conditions will be more harsh than that in this study. ,, Compared with rice straw pretreatment with NaOH, the biphasic EPH system also presented the advantages of moderate conditions and less water consumption . Moreover, in consideration of the excellent characteristic of the biphasic system on products separation, the EPH/water system provides a way for more efficient fractionation of RS.…”

Section: Resultsmentioning

confidence: 77%

“…26,29,30 Compared with rice straw pretreatment with NaOH, the biphasic EPH system also presented the advantages of moderate conditions and less water consumption. 31 Moreover, in consideration of the excellent characteristic of the biphasic system on products separation, the EPH/water system provides a way for more efficient fractionation of RS.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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A Novel Recyclable Alkaline Biphasic 2-Phenoxyethanol/Water System for Rice Straw Biorefinery under Mild Conditions

Zhang

Tan

Wen

et al. 2020

ACS Sustainable Chem. Eng.

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A novel recyclable alkali-assisted biphasic pretreatment process under mild conditions with low water consumption is first developed. After conditions were optimized with the uniform design method, the biphasic 2-phenoxyethanol system presented superior delignification performance with only 6 mL of water consumption per gram of rice straw. Namely, the lignin removal reached 82.16% at 80 °C or 78.15% even at 55 °C. Then, according to the characteristics of the biphasic system, the entire solvent circulation process was constructed. The recycled 2phenoxyethanol and washing wastewater were directly reused three times at 80 °C, giving more than 80% of lignin removal. Meanwhile, enzymatic digestibility of corresponding residues still exceeded 80% in acetate buffer, or 71% even in black liquor at 72 h. These show the possibility of constructing a biorefinery system based on an alkaline biphasic pretreatment process, with less water and energy consumption, fractionable composition, solvent recyclability, and black liquor utilization.

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Section: Resultsmentioning

confidence: 77%

Section: ■ Results and Discussionmentioning

confidence: 99%

A Novel Recyclable Alkaline Biphasic 2-Phenoxyethanol/Water System for Rice Straw Biorefinery under Mild Conditions

Zhang

Tan

Wen

et al. 2020

ACS Sustainable Chem. Eng.

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“…This should most likely be related to the highly reduced state of glucose and fructose in addition to glycerol, which unlike the other sugars, promotes the generation of SA [34]. Prior studies also pointed out a high yield in SA production when mutant bacterial strains were used [4,35,36]. The feasibility of SA production via A. succinogenes co-utilizing glucose and fructose simultaneously as carbon sources was evaluated.…”

Section: Results From 250 ML Anaerobic Bottles Using Sole and Mixed Sugars As Carbon Source In Anaerobic Conditionsmentioning

confidence: 99%

Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production

Salma

Djelal

Abdallah

et al. 2021

Applied Microbiology

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The anaerobic fermentation of glucose and fructose was performed by Actinobacillus succinogenes 130Z in batch mode using three different volume of bioreactors (0.25, 1 and 3 L). The strategy used was the addition of MgCO3 and fumaric acid (FA) as mineral carbon and the precursor of succinic acid, respectively, in the culture media. Kinetics and yields of succinic acid (SA) production in the presence of sugars in a relevant synthetic medium were investigated. Work on the bench scale (3 L) showed the best results when compared to the small anaerobic reactor's succinic acid yield and productivity after 96 h of fermentation. For an equal mixture of glucose and fructose used as substrate at 0.4 mol L−1 with the addition of FA as enhancer and under proven optimal conditions (pH 6.8, T = 37 °C, anaerobic condition and 1% v/v of biomass), about 0.5 mol L−1 of SA was obtained, while the theoretical production of succinic acid was 0.74 mol L−1. This concentration corresponded to an experimental yield of 0.88 (mol-C SA/mol-C sugars consumed anaerobically) and a volumetric productivity of 0.48 g-SA L−1 h−1. The succinic acid yield and concentration obtained were significant and in the order of those reported in the literature.

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“…Lignocellulosic biomass, mainly comprising of cellulose, hemicellulose and lignin, are the most abundant, sustainable, and cost-effective types of biomass for butanol production. 39,40 Among them, cellulose is an unbranched homopolysaccharide consisting of D-glucopyranosyl units that can be broken down to hexoses (C-6 sugars), and hemicellulose are branched heteropolysaccharides consisting of both hexose and pentose sugar residues. 41,42 Lignin consists of phenylpropane units linked through different types of interunit linkages, playing a cementing role to connect cells and increasing the mechanical strength properties, which makes lignocellulosic resistant against biodegradation by microorganisms.…”

Section: Substrates For Lignocellulosic Butanolmentioning

confidence: 99%

Production of butanol from lignocellulosic biomass: recent advances, challenges, and prospects

et al. 2022

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Optimization of sodium hydroxide pretreatment and enzyme loading for efficient hydrolysis of rice straw to improve succinate production by metabolically engineered Escherichia coli KJ122 under simultaneous saccharification and fermentation

Cited by 22 publications

References 40 publications

A Novel Recyclable Alkaline Biphasic 2-Phenoxyethanol/Water System for Rice Straw Biorefinery under Mild Conditions

A Novel Recyclable Alkaline Biphasic 2-Phenoxyethanol/Water System for Rice Straw Biorefinery under Mild Conditions

Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production

Production of butanol from lignocellulosic biomass: recent advances, challenges, and prospects

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