Optimization of solid-state fermentation for bioherbicide production by Phoma sp .

Abstract

-In this work the bioherbicide production from Phoma sp. by solid-state fermentation was optimized. Agroindustrial residues such as bagasse, soybean bran and corn steep liquor were used as substrate. The bioherbicide was extracted from the fermented solid and the supernatant was applied for the control of the target plant. The evaluated responses in the bioassay were plant height, root length, fresh and dry weight, number of flowers and phytotoxicity. The results in the bioassays demonstrated that the bioherbicide presented activity towards the target plant and the intensity of the effect was influenced by the formulation of the fermentation medium. The optimized condition for bioherbicide production was (wt%): moisture content 70.0, soybean bran content 30.0 and corn steep liquor (CSL) 20.0, it being possible to obtain an injury phytotoxicity level of 40. The bioherbicide showed a mode of action based on the inhibition of carotenoid biosynthesis.

Bioherbicidal action of Phoma dimorpha fermented broth on seeds and plants of Senna obtusifolia 1

“…A significant difference (p < 0.05) was observed for plant height with the application of the P. dimorpha fermented broth on S. obtusifolia plants, resulting in a lower height (Table 4). Similar results were observed by Klaic et al (2017), who found a reduction of 30 % in the plant height of the bioindicator C. sativus, when submitted to the same fermented broth.…”

“…This reduction, as a function of the fermented broth, was also observed by Brun et al (2016) on C. sativus plants at 7 days after the application of Phoma sp. Similarly, Klaic et al (2017), after optimizing the solid state fermentation process for the production of the bioherbicide through Phoma sp. secondary metabolites, also observed its phytotoxicity on C. sativus plants, with a reduction of 20 % in the fresh mass, in comparison to the control treatment.…”

“…For the fermentation process, Petri dishes containing the active growing fungus were kept in a bacteriological oven for 7 days, at 28 ºC, from which 2 mycelium disks of 6 mm were removed and transferred to Erlenmeyer flasks with 130 mL of a liquid medium containing potato extract (200 g L -1 ), dextrose (20 g L -1 ), peptone (10 g L -1 ), yeast extract (7.5 g L -1 ), ammonium sulfate [(NH 4 ) 2 SO 4 ; 2 g L -1 ], ferrous sulfate (FeSO 4 .7H 2 O; 1g L -1 ) and magnesium sulfate (MgSO 4 ; 0.5 g L -1 and MnSO 4 .H 2 O; 1g L -1 ) (Zhang et al 2012), previously sterilized by autoclaving at 121 ºC, for 30 min. The flasks were maintained at 28 ºC, under agitation of 120 rpm, for 10 days (Innova 44R, New Brunswick) (Klaic et al 2017). After this period, the cells were separated from the fermented broth through a filter paper with 14 μm pores in vacuum filtration with a e-ISSN 1983-4063 -www.agro.ufg.br/pat -Pesq.…”

Efeito de metabólitos secundários produzidos por Phoma dimorpha sobre a germinação e crescimento de sementes de diferentes espécies vegetais

“…1 g L -1 Fonte: Para et al 2005 Em seguida, os frascos foram mantidos a temperatura de 28°C e agitação de 120 rpm (Innova 44R, New Brunswick) por um período de 10 dias, de acordo com metodologia descrita por Klaic et al (2017) (Brasil, 2009).…”

Production and Stabilization of Mycoherbicides

“…Naturally occurring substances can be applied for bioherbicide production [106,107]. SSF allows to obtain bioherbicides utilizing the agroindustry waste such as bagasse, soybean bran and corn steep liquor [108].…”