Optimization of solid state medium for the production of clavulanic acid by Streptomyces clavuligerus

Sircar, Anindya; Sridhar, P.; Das, Parichay K.

doi:10.1016/s0032-9592(97)00058-7

Cited by 34 publications

(26 citation statements)

References 5 publications

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“…Because of the above disadvantages, Solid state fermentation (SSF) has emerged as an alternate cost-effective process for bioproducts production by utilizing the agrowastes and byproducts [19]. It has been employed for production of various microbial metabolites such as enzymes [3,4,20], organic acids [21][22][23] and antibiotics [24,25]. Optimization of bioprocess by one factor at a time (OFAT) is a wellstudied method but it has some disadvantages like more time consumption, more number of experimental runs and lack of knowledge about the interaction between the variables involved in the process [26].…”

Section: Introductionmentioning

confidence: 99%

Optimization of L-asparaginase production by Serratia marcescens (NCIM 2919) under solid state fermentation using coconut oil cake

Ghosh

Murthy

Sharmila

et al. 2013

sustain chem process

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Background: The present study focused on utilization of agrowaste byproducts generated from oil mill for L-asparaginase enzyme production using Serratia marcescens under solid state fermentation. Classical and statistical methods were employed to optimize the process variables and the results were compared. Results: The classical one factor at a time (OFAT) and response surface methodology (RSM) are employed to optimize the fermentation process. When used as the sole carbon source in SSF, coconut oil cake (COC) showed maximum enzyme production. The optimal values of substrate amount, initial moisture content, pH and temperature were found to be 6 g, 40%, 6 and 35°C respectively under classical optimization method with maximum enzyme activity of 3.87 (U gds -1 ). Maximum enzyme activity of 5.86 U gds -1 was obtained at the predicted optimal conditions of substrate amount 7.6 g of COC, initial moisture content of substrate 50%, temperature 35.5°C and pH 7.4. Validation results proved that a good relation existed between the experimental and the predicted model. Conclusions: RSM optimization approach enhances the enzyme production to 33% when compared to classical method. Utilization of coconut oil cake as a low cost substrate in SSF for L-asparaginase production makes the process economical and also reduces the environmental pollution by converting the oil mill solid waste into a useful bioproduct.

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Section: Introductionmentioning

confidence: 99%

Optimization of L-asparaginase production by Serratia marcescens (NCIM 2919) under solid state fermentation using coconut oil cake

Ghosh

Murthy

Sharmila

et al. 2013

sustain chem process

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“…By a fractional factorial design, the significant factors and their effects can be studied using only a few experiments, thus reducing the operating costs [11]. Most of previous studies were devoted to the improvement of CA production by S. clavuligerus [6], by selection of the optimum operating conditions, namely pH [12], temperature [13], agitation [14], concentrations of glycerol as a carbon source [8], and of soybean flour as a nitrogen source [15].…”

Section: Introductionmentioning

confidence: 99%

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Viana

Carneiro-Cunha

Araújo

et al. 2009

Appl Biochem Biotechnol

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Clavulanic acid (CA) is a beta-lactam antibiotic, which has a potent beta-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28 degrees C, 30 degrees C, and 32 degrees C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32 degrees C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g(X)/g(S) and 64.3 mg(P)/g(S), respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%.

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“…Yang and Swei ( 1996 ) utilised corncob, as SSF substrate in the production of oxytetracycline by Streptomyces rimosus , supplemented with 20 % (w/w) rice bran or 1.5-2.5 % ammonium sulphate and showed a yield of 10-11 mg/g substrate. Sircar et al ( 1998 ) used supplementary sources including soya fl our, KH 2 PO 4 and sunfl ower oil cake and improved the production of clavulanic acid by S. clavuligerus . The maximum productivity of cephalosporin C (22,281 µg/g) employing A. chrysogenum ATCC 48272 was achieved by utilising wheat rawa with optimised process parameters including 1 % w/w soluble starch and 1 % w/w yeast extract as additives (Adinarayana et al 2003 ).…”

Section: Selection Of Supplementsmentioning

confidence: 99%

Solid-State Fermentation of Agricultural Residues for the Production of Antibiotics

Kumar

Selvaraj

Dharani

et al. 2013

Biotransformation of Waste Biomass Into High Value Biochemicals

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Optimization of solid state medium for the production of clavulanic acid by Streptomyces clavuligerus

Cited by 34 publications

References 5 publications

Optimization of L-asparaginase production by Serratia marcescens (NCIM 2919) under solid state fermentation using coconut oil cake

Optimization of L-asparaginase production by Serratia marcescens (NCIM 2919) under solid state fermentation using coconut oil cake

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Solid-State Fermentation of Agricultural Residues for the Production of Antibiotics

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