Optimization of soybean hull acid hydrolysis and its characterization as a potential substrate for bioprocessing

Cassales, A. R.; Souza-Cruz, Priscila Brasil de; Rech, Rosane; Ayub, Marco Antônio Záchia

doi:10.1016/j.biombioe.2011.09.021

Cited by 52 publications

(36 citation statements)

References 27 publications

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“…The total nitrogen and ammonia nitrogen presenting in the corncob hydrolysate were detected by Hach Water Quality Analyzer DR2700. Soluble lignin in corncob hydrolysate was estimated according to Cassales et al (2011).…”

Section: Methodsmentioning

confidence: 99%

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum

et al. 2012

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Corncob was treated by dilute H(2)SO(4). The hydrolysate contained 45.7 g sugar/l. Without concentration or adding other nutrients, the hydrolysate, after being detoxified by overliming and adsorption with activated charcoal, was used for oil production using Trichosporon cutaneum. After 8 days' growth in shake-flasks, the biomass was 22.1 g/l with a lipid content of 36%. The lipid yield per mass of sugar was 17.4% (w/w). Corncob thus is a promising raw material for microbial oil production by this yeast.

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Section: Methodsmentioning

confidence: 99%

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum

et al. 2012

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“…Los parámetros cinéticos se estiman empleando diferentes experiencias reportadas en literatura (PURWADI; NIKLASSON;TAHERZADEH, 2004;GUTIERREZ-PADILLA;KARIM, 2005;CASSALES et al, 2011). Los resultados numéricos presentados muestran un ajuste satisfactorio entre los perfiles logrados y los datos experimentales, concluyendo que los modelos propuestos para las etapas de hidrólisis ácida diluida, detoxificación y cofermentación son capaces de describir el comportamiento de los datos experimentales utilizados.…”

Section: Conclusionesunclassified

“…Los modelos propuestos se ajustan empleando datos experimentales de bibliografía (PURWADI; NIKLASSON;TAHERZADEH, 2004;CASSALES et al, 2011;GUTIERREZ-PADILLA;KARIM, 2005). El problema dinámico de estimación de parámetros, formulado para cada una de las etapas mencionadas, se modela como un sistema DAE que luego se convierte a un …”

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Producción de Etanol a Partir de Biomasa Lignocelulósica: Estimación Dinámica de Parámetros con Enfoque Simultáneo

Andersen

Moreno

Díaz

2013

IJIE

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RESUMEN:En este trabajo se lleva a cabo la estimación de parámetros en modelos cinéticos correspondientes a las diferentes operaciones involucradas en la producción bioquímica de etanol lignocelulósico. El modelo resultante se representa con un sistema de ecuaciones algebraico-diferenciales (DAE), que incluye las principales variables del proceso. El problema de estimación de parámetros se resuelve mediante un enfoque simultáneo, en el que el DAE se convierte en un sistema algebraico, mediante la técnica de colocación ortogonal sobre elementos finitos. Así, estimar los parámetros del modelo implica minimizar una función objetivo de cuadrados mínimos ponderados sujeta a restricciones algebraicas. Los modelos cinéticos se pueden emplear en futuros trabajos de diseño y simulación de plantas productoras de etanol. En cada modelo se lograron ajustes satisfactorios de los datos experimentales obtenidos de bibliografía.Palabras clave: Estimación de parámetros. Optimización dinámica. Colocación ortogonal. Etanol lignocelulósico. INTRODUCCIÓNLa biomasa lignocelulósica es la más abundante y económica de las materias primas en la naturaleza utilizadas para la producción del denominado bioetanol de segunda generación.La misma contiene una mezcla heterogénea de biopolímeros proveniente de la pared celular

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“…Interestingly, compared with other waste lignocellulosic biomass, soybean hulls typically contain 6–15% pectin and are poorly lignified, which contributes to raising the question whether this by‐product generated massively in America could be exploited for pectinases production. Recently, substantial attention has been paid to different pretreatment methods, such as physico‐chemical or enzymatic hydrolysis, to yield fermentable carbohydrates for bioethanol production . Enzyme production from soybean hull has received some attention, although research was directed almost entirely to cellulases and xylanases .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, substantial attention has been paid to different pretreatment methods, such as physico-chemical or enzymatic hydrolysis, to yield fermentable carbohydrates for bioethanol production. [10][11][12] Enzyme production from soybean hull has received some attention, although research was directed almost entirely to cellulases and xylanases. 9 The rationale behind this approach lies in the relatively higher content of cellulose and hemicellulose compared with pectin in soybean hulls, 10 which is in turn apparent due to the nature of the by-product.…”

Section: Introductionmentioning

confidence: 99%

Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine‐tuning of ambient pH

Fratebianchi¹,

Acosta²,

Cavalitto³

2017

J of Chemical Tech & Biotech

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BACKGROUND Soybean hulls result from the processing of the bean for producing oil and protein products. This by‐product generated massively in America has virtually no commercial value, so substantial effort is being applied to its exploitation for generating value‐added goods. This work evaluates soybean hulls as inducer of the production of pectinolytic enzymes, through optimization studies regarding polygalacturonase production by Aspergillus sojae in submerged cultures. RESULTS A 2‐fold improvement in polygalacturonase yield was found by varying the initial pH of the culture in a very narrow acid pH range (2.40–2.80). The optimized fermentation process was successfully transferred to stirred‐tank bioreactors in terms of volumetric productivity, and final polygalacturonase yields were 42 U mL‐1 and 1.39 U g‐1 soybean hulls, which are among the highest reported with this by‐product. Morphological characterization of A. sojae during cultivation showed that the fungus mainly developed in dispersed mycelia at initial pH of 2.40–2.80 while, conversely, fungal pellets predominated in cultures performed at initial pH of 5.40. CONCLUSION High enzyme titers are possibly connected to the formation of dispersed mycelia, as well as to acid‐induced expression of the respective gene/s. It is foreseen that this data will be helpful regarding the production of fungal pectinases or other acid‐induced enzymes. © 2017 Society of Chemical Industry

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Optimization of soybean hull acid hydrolysis and its characterization as a potential substrate for bioprocessing

Cited by 52 publications

References 27 publications

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum

Producción de Etanol a Partir de Biomasa Lignocelulósica: Estimación Dinámica de Parámetros con Enfoque Simultáneo

Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine‐tuning of ambient pH

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