Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum

Abstract

To facilitate Ganoderma lucidum submerged cultivation and achieve high productivity, four fine powder solid substrates incorporated with different nitrogen-rich supplements were utilized to grow the fungus and as solid seed for its submerged culture. Of the four solid seeds, the soybean meal solid seed gave the highest biomass (10.73 g/ L) and exopolysaccharide (EPS) (1.22 g/L), higher than those (8.36 g/L biomass and 0.44 g/L EPS) obtained with mycelial liquid seed. The optimal level of soybean meal supplementation was 20% (w/w) for production of the solid seed. Following single factor experiments, levels of three selected process variables were optimized as: the moisture content of solid seed, 70%; inoculum size, 0.8 g/flask; and rotary speed, 160 rpm. These conditions were validated experimentally with improved EPS yield of 1.33 g/L. The developed solid seed can be conveniently used for G. lucidum submerged culture with improved EPS productivity.

Flexible Fungal Materials: Shaping the Future

“…With both a tensile Box 3. Liquid-State (Surface) Fermentation for Paper-like Materials LSF methods are associated with shorter cultivation periods, scalability, and lower space requirements [82,83], making them well suited to produce compact fungal pellets [5,84], which can then be converted into pulp for paper-like materials. Separation of fungal biomass and substrate is easily achieved in LSF through filtration, thus facilitating easy harvesting of pure mycelium as a source of chitin or chitosan [85].…”

Aspects of mushroom cultivation to obtain polysaccharides in submerged cultivation

“…In flask cultivation, to develop effective and economical solid seeds suitable for use in submerged culture for the production of EPS, four supplements were examined as sources of nitrogen for the formulation of substrate with sawdust as the basal ingredient. The higher biomass and EPS yield obtained for the four solid test seeds on the liquid seed were attributed to the formation of a large number of growth points of hyphae in cultures, resulting from the dissociation of inoculated solid seeds into numerous tiny particles (Liu and Zhang, 2019).…”

“…In addition to solid-state crops, fungi are grown in liquid media in bottles or bioreactors. This type of cultivation presents a fast, efficient and less expensive alternative form (Wu et al, 2003(Wu et al, , 2004Tang and Zhong, 2002), in which organisms can be obtained in limited physical spaces under conditions controlled and optimized for the production of biomass, exopolysaccharides (EPS) and endopolysaccharides (IPS), simultaneously (Cui et al, 2006;Confortin et al, 2008;Liu and Zhang, 2019;Guillén-Navarro et al, 1998), also showing the ease of separating the mycelium from the culture medium, obtaining products of uniform quality, in addition to the possibility of being cultivated all year round (Leonowicz et al, 1991;Rosado et al, 2002).…”

Current Progress Regarding Cordyceps militaris, Its Metabolite Function, and Its Production

“…Liquid culture is the other option for C. militaris cultivation. It is considered a better procedure for application in industry due to its shorter cultivation times, smaller space requirements, and higher productivities [163]. Cordyceps militaris is allowed to grow in liquid as mycelia, without forming fruiting bodies, and total cultivation times can decrease from 60 to 15 days [164].…”