Optimization of tannase production by a novel Klebsiella pneumoniae KP715242 using central composite design

Kumar, Manoj; Rana, Shiny; Beniwal, Vikas; Salar, Raj Kumar

doi:10.1016/j.btre.2015.06.002

Cited by 29 publications

(13 citation statements)

References 23 publications

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“…The yeast isolation and screening were performed as previously reported [21]. As for the screening of tannin-utilizing yeast strains, the visual reading method was executed according to Kumar, with some modifications [22]. A single yeast colony was picked and inoculated the plate of the test medium at 28 °C for 3 days, then green to brown coloration of the medium was judged as a positive result for tannin degradation.…”

Section: Methodsmentioning

confidence: 99%

Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9

Zhang

Wang

et al. 2019

Biomolecules

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Tannins biodegradation by a microorganism is one of the most efficient ways to produce bioproducts of high value. However, the mechanism of tannins biodegradation by yeast has been little explored. In this study, Aureobasidium melanogenum T9 isolated from red wine starter showed the ability for tannins degradation and had its highest biomass when the initial tannic acid concentration was 20 g/L. Furthermore, the genes involved in the tannin degradation process were analyzed. Genes tan A, tan B and tan C encoding three different tannases respectively were identified in the A. melanogenum T9. Among these genes, tan A and tan B can be induced by tannin acid simultaneously at both gene transcription and protein expression levels. Our assay result showed that the deletion of tanA and tanB resulted in tannase activity decline with 51.3 ± 4.1 and 64.1 ± 1.9 U/mL, respectively, which is much lower than that of A. melanogenum T9 with 91.3 ± 5.8 U/mL. In addition, another gene coding gallic acid decarboxylase (gad) was knocked out to better clarify its function. Mutant Δgad completely lost gallic acid decarboxylase activity and no pyrogallic acid was seen during the entire cultivation process, confirming that there was a sole gene encoding decarboxylase in the A. melanogenum T9. These results demonstrated that tanA, tanB and gad were crucial for tannin degradation and provided new insights for the mechanism of tannins biodegradation by yeast. This finding showed that A. melanogenum has potential in the production of tannase and metabolites, such as gall acid and pyrogallol.

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Section: Methodsmentioning

confidence: 99%

Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9

Zhang

Wang

et al. 2019

Biomolecules

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“…They contain the bacteria: Bacillus sp. PAB2, B. licheniformis KBR6 and Klebsiella pneumoniae KP715242 (Jana et al, 2012 andKumar et al, 2015); yeast: Kluyveromyces marxianus NRRL Y-8281 and Saccharomyces cerevisiae CCMB 520 (Fathy et al, 2017 andMorgana de Melo Lopes et al, 2017) and fungi:…”

Section: Introductionmentioning

confidence: 99%

Implementation of Different Fermentation Techniques for Induction of Tannase and Gallic Acid Using Agro-residues Substrates

Ahmed

Abou-Taleb

2019

Egypt. J. Microbiol.

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T ANNASE is an inducible enzyme which hydrolysate tannin to gallic acid and used to in many industries such as food and pharmaceutical. This enzyme has scooped more attention in recent times. A maximum production of tannase (ranged from 0.30 to 0.93 enzyme index and from1.05 to 1.87U/mg specific tannase activity (STA)) and gallic acid (from 0.07 to 0.76mg/ml gallic acid concentration (GAC)) was achieved by 8 fungal strains out of 24 fungal and yeast strains belonged to genera; Aspergillus, Rhizopus, Trichoderma, Fusarium, Penicillium, Candida and Saccharomyces. The selected fungi were grown on tannin-rich substrates (eucalyptus leaves, pomegranate peel, banana peel, guava leaves and wheat bran) and by-products (corn steep liquor (CSL) and soybean extract) as sole carbon and nitrogen sources for tannase and GA production at 28°C for 6 days under liquid-surface (LSF), submerged (SmF) and solid-state (SSF) fermentation. Results indicated that A. niger A8 and T. viride with 10% (v/v) of inoculum size gave a high STA (from 8.08 to 10.95U/mg) and GAC (from 2.62 to 4.00mg/ml) on pomegranate and banana peels supplemented with CSL after 4 days incubation at 28°C under SSF compared to SmF and LSF. STA (ranged from 10.68 to 12.93U/mg) and GAC (ranged from 3.56 to 4.16mg/ml) were increased when inoculated the medium with both A. niger A8+T. viride (with 5:5% v/v of inoculum size) more than when inoculated with each of them separately.

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“…whereas tannase activity pH optima for Bacillus cereus KBR9 and Bacillus licheniformis KBR 6 was 4.5 and pH 5.75 respectively (Mondal and Pati 2000;Rodríguez et al 2008). Several other investigators have reported the optimal pH from 4 to 5 (Gayen and Ghosh 2013;Bagga et al 2015;Kumar et al 2015;Farag et al 2018). However, some reports also indicated the activity in alkaline range (Iwamoto et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Corn (Zea Mays) Leaves as Cost-Effective Substrate for Enhanced Tannase Production Under Solid State Fermentation: Optimization and Characterization

Shakir

Yousaf

Ali

et al. 2021

Preprint

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The current study reports the utilization of agricultural residues of corn (Zea mays) as lower-cost substrate for the production of tannase under solid state fermentation (SSF). Tannase producing bacterial strains were isolated from gut content of freshwater fish, Ctenopharyngodon idella, and highest tannase producer was identified as Bacillus cereus using 16S rDNA sequencing. For enhanced tannase production, B. cereus was investigated using one variable at a time (OVAT) followed by central composite design (CCD) of response surface methodology (RSM). Under OVAT, optimal fermentation conditions and medium composition were achieved with 70% substrate moisture, distilled water (1 ml) as enzyme extraction medium, 30°C incubation temperature, 3.0 pH, 1% inoculum size, 24 h incubation time, 150 rpm agitation, large-sized substrate particles (4 mm), non-centrifugation condition, NaCl salt, 1.5% tannic acid substrate and malt extract as organic nitrogen source for maximal enzyme synthesis. The highest tannase was produced (155.74±1.67 U/ml) with 1.75% tannic acid, 0.75% NaCl and 1.25% malt extract with the application of CCD of RSM. Higher coefficient of regression (R2=0.9665) indicated that second-order polynomial regression model assessed the data excellently. Further, tannase characterization depicted its maximum activity at 5.0 pH, 50°C temperature, 45 min incubation and 0.35% tannic acid.

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Optimization of tannase production by a novel Klebsiella pneumoniae KP715242 using central composite design

Cited by 29 publications

References 23 publications

Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9

Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9

Implementation of Different Fermentation Techniques for Induction of Tannase and Gallic Acid Using Agro-residues Substrates

Corn (Zea Mays) Leaves as Cost-Effective Substrate for Enhanced Tannase Production Under Solid State Fermentation: Optimization and Characterization

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