Optimization of Technologies for Manufacture of Demineralized Bone Implants for Drug Release

Litvinov, Yu. Yu.; Матвейчук, Ю. В.; Розанов, В. В.; Краснов, В. В.

doi:10.1007/s10527-021-10047-5

Cited by 3 publications

(2 citation statements)

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“…Reconstruction involves bone implants placed in the defect area. A wide range of materials is used in implant manufacture (metals, polymer composites, ceramics, and coral) [7][8][9]; however, bone tissue remains preferable as it provides a number of advantages: The use of autogenic bone prevents immunologic havoc. In addition, the bone is biologically active as it is capable of initiating regenerative reactions.…”

Section: Introductionmentioning

confidence: 99%

Compressive Strength Characteristics of Long Tubular Bones after Hyperthermal Ablation

et al. 2022

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Thermoablation is used in the treatment of tumorous bones. However, little is known about the influence such thermal treatment has on the mechanical properties of bone tissue. The purpose of this work was to study the influence of thermal treatment in a range of 60–100 °C (in increments of 10 °C) on the structural properties of pig femurs using an original approach that involved a periosteal arrangement of heating elements providing gradual dry heating of the bone from its periphery to its center. Heating of freshly extracted bone tissue segments was performed ex vivo using surface heaters of a Phoenix-2 local hyperthermia hardware system. Mechanical testing followed the single-axis compression scheme (traverse velocity of 1 mm/min). In the 60–90 °C range of heating, no attributes of reduced structural characteristics were found in the samples subjected to thermoablation in comparison to the control samples taken from symmetric areas of adjacent cylinders of healthy bones and not subjected to heat treatment. The following statistically significant changes were found as a result of compressing the samples to 100 °C after the heat treatment: reduced modulus of elasticity and increased elastic strain (strain-to-failure), mainly due to increases in plastic deformation. This finding may serve as evidence of a critical ex vivo change in the biomechanical behavior of bone tissues at such temperatures. Thus, ex vivo treatment of bone tissue in the thermal range of 60–90 °C may be used in studies of thermoablation efficiency against tumor involvement of bones.

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Section: Introductionmentioning

confidence: 99%

Compressive Strength Characteristics of Long Tubular Bones after Hyperthermal Ablation

et al. 2022

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“…Biomaterials used in reconstructive surgery are available in a rather wide range of artificial (metals, polymers, ceramics, etc. ), natural (bone allotransplants, autotransplants), and composite osteoplastic materials [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Mechanical and Histological Characteristics of Human Tubular Bones after Hyperthermal Treatment

et al. 2023

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This research focused on studying regularities in changes in strength characteristics and histological patterns of healthy tubular bone tissue depending on the temperature setting of hyperthermal treatment. Experimentation has established that heating the experimental bone sample in a temperature range of 60 to 70 °C does not cause any decline in strength characteristics compared to the control samples not subject to heat treatment. In compression tests (along the length of the bone), after heating the bone samples ex vivo to 80 °C, the strength characteristics were found to increase as the samples sustained a higher maximum stress. In bending tests, in contrast, the strength characteristics were reliably found to decrease in bone samples at 80 °C and 90 °C for the maximum stress indicator and 90 °C for the modulus of elasticity. Data obtained through histological examination further demonstrated statistically significant differences between the two temperature ranges of 60–70 °C and 80–90 °C, where semi-quantitative assessment revealed statistically significant differences in the markers of bone tissue destruction caused by hyperthermal treatment. Moderate (at 60–70 °C) and pronounced (at 80–90 °C) dystrophic and necrotic changes were observed both in the cells and the intercellular matrix of the tibia. From a practical point of view, the temperature range of 60–70 °C can be considered operational for thermal ablation since, at these temperatures, no statistically significant decline was observed for the strength characteristics in either the cross-section or length-section.

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Evaluation of Biocompatibility and Adhesive Properties of Scaffolds Based on Inorganic Bone Matrix

Litvinov,

Kabanov,

Krasnov

2024

Problems Bio Med Pharm Chem

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Introduction. The use of cellular engineering structures, including cell cultures and composite biomaterials, ensures maximum efficiency of bone de-fect repair, and the development of techniques and testing of carriers for osteogenic cell transplantation remains an urgent task of modern science. The study carried out biological testing of scaffolds based on the inorganic bone matrix obtained by a previously developed and patented method at the Institute of Medicinal and Aromatic Plants using human fibroblasts in terms of cytotoxicity, efficiency of cell adhesion and proliferation. The purpose of the study is to evaluate experimentally the biocompatibility and adhesive properties of SIBM samples using human cell cultures in vitro to create an implantation cellular engineering structures. Material and methods. The object of the study is SIBM samples of the inorganic bone tissue of the compact substance of the diaphysis of the fe-mur of a bull with a cell line of human dermal fibroblasts HdFb (d77). The biocompatibility and adhesive properties of SIBM with respect to the cell line of dermal fibroblasts, their effect on cell viability and proliferative activity were studied using phase contrast microscopy, scanning electron microsco-py, and the classical spectral method using tetrazolium yellow salt (MTT method). Results. The formation of contacts of the plasma membrane of dermal fibroblasts with the SIBM samples was studied within 240 hours of incubation. At the same time, there were no significant changes in cell morphology. They formed a confluent monolayer (with cells tightly adjacent to each other), adhered well and were distributed over the surface of the SIBM. SIBM samples had virtually no effect on the metabolic activity of HdFb cells. The data obtained indicate a minimal effect of the studied samples on cell viability and proliferation, the absence of toxicity to cells and good biocompatibility with dermal fibroblasts in vitro. Conclusions. It has been experimentally proved that the studied SIBM samples obtained by the previously developed method at the Institute of Me-dicinal and Aromatic Plants have adhesive properties, lack of cytotoxicity with respect to human HdFb dermal fibroblasts and have little effect on their proliferation in vitro.

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Optimization of Technologies for Manufacture of Demineralized Bone Implants for Drug Release

Cited by 3 publications

References 1 publication

Compressive Strength Characteristics of Long Tubular Bones after Hyperthermal Ablation

Compressive Strength Characteristics of Long Tubular Bones after Hyperthermal Ablation

Mechanical and Histological Characteristics of Human Tubular Bones after Hyperthermal Treatment

Evaluation of Biocompatibility and Adhesive Properties of Scaffolds Based on Inorganic Bone Matrix

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