Optimization of temperature and light intensity for improved photofermentative hydrogen production using Rhodobacter capsulatus DSM 1710

Investigation of the effects of initial substrate and biomass concentrations and light intensity on photofermentative hydrogen gas production by Response Surface Methodology

“…Regarding the effect of the light intensity value, optimum light intensity of 263.6 W/m 2 (3955 lux) found in this study is close to the optimal range of 4000e6000 lux reported in other studies [15,17,29,30]. Androga et al [11] stated that 285 and 287 W/m 2 are the optimum light intensity for hydrogen production. Shi and Yu [17] studied the effect of light intensity considering the interactive effect of cell concentration.…”

“…The light intensities on the photobioreactors' surfaces were measured by a luxmeter (Lutron). The amount of the evolved gas was determined volumetrically by using water replacement method [11]. Gas composition was analyzed by a Gas Chromatograph (GC), (Agilent Technologies 6890N).…”

“…Temperature and light intensity are two of these factors which strongly affect the hydrogen production by PNS bacteria [11]. Optimal temperature for PNS bacteria to grow and produce hydrogen is between 30 and 35 C [12].…”

“…One way of researching this combined (interactive) effect can be achieved via experimental design methods such as Response Surface Methodology (RSM). To our knowledge, the studies investigating this combined effect of parameters on photofermentative H 2 production are very limited [11,17]. In addition, these studies only focus on two parameters.…”

Statistical optimization of simultaneous saccharification fermentative hydrogen production from corn stover

“…Three-dimensional response surface and contour maps were used to determine the interaction between fermentation conditions and optimal reaction conditions for hydrogen production in synchronous saccharification fermentation. The color and shape of the contour line can determine the significance of the variable and the interaction between variables, with larger color changes and gradients indicating a greater impact on the experiment, an elliptical contour, indicating a strong mutual effect, while a circular contour indicates no interaction [35][36][37][38]. Figure 1 shows the three-dimensional response surface diagram and contour diagram of the effects of hydrogen yield on the hydrogen production in synchronous saccharification fermentation as a function of substrate concentration, inoculum, pH, and enzyme concentration.…”