Optimization of the antioxiant peptides production from goat milk casein hydrolyzed by alcalase and papain using respons surface methodology

Abstract

Antioxidant peptides are widely used in many fields due to their biological activities such as anti-cancer, anti-induction and anti-aging. However, little information is available concerning the peptides production by enzymatic hydrolysis of casein from goat milk. In this study, response surface methodology (RSM) was applied to optimize the conditions of the hydrolysis of goat milk casein by proteases (alcalase in combination with papain) to increase the antioxidant activity of the peptides. The results showed that the optimal hydrolysis conditions were as follows: temperature of 61°C, enzyme/substrate ratio (E/S) of 5.6%, and protease ratio (papain: alcalase) of 1.8:1. Under the optimal conditions, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity significantly increased (1.17 folds compared to un-optimized conditions). Furthermore, Fe 2+ chelating ability and superoxide radical scavenging activity increased 1.02 folds and 1.14 folds, respectively. The antioxidant activity was significantly improved after optimization and provided a basis for the study of the antioxidant effects of hydrolysed goat milk casein.

Use of Alcalase in the production of bioactive peptides: A review

“…It was found that the hydrolysis conditions that led the highest antioxidative activity of the produced peptides were 55°C, pH 7.5, substrate concentration of 3.0%, an enzyme/substrate of 4.0%, a ratio of Alcalase/papain of 1/3 and a reaction time of 180 min [409]. Later, the optimization of hydrolysis condition of goat milk casein using mixtures of Alcalase/papain was optimized via response surface methodology [410]. The optimal reaction conditions were 61°C, enzyme/substrate ratio of 5.6%, and a combi-protease papain: Alcalase of 1.8.…”

“…The optimal reaction conditions were 61°C, enzyme/substrate ratio of 5.6%, and a combi-protease papain: Alcalase of 1.8. This led a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity increased 1.17 folds compared to un-optimized conditions [410].…”

The optimization of peptides – producing conditions for antioxidant peptides in goat milk by Lactobacillus casei L61

“…After centrifugation at 5000×g for 15 minutes, the supernatant was collected and adjusted to pH 8.3 with NaOH (1 mol/L) and centrifuged at 5000×g for 15 minutes. The supernatant was collected as the whey sample for the determination of antioxidant activity (Shu et al, 2019).…”