Optimization of the cultural medium and conditions for production of antifungal substances by Streptomyces platensis 3-10 and evaluation of its efficacy in suppression of clubroot disease ( Plasmodiophora brassicae ) of oilseed rape

Shakeel, Qaiser; Lyu, Ang; Zhang, Jing; Wu, Mingde; Chen, Shouwen; Cao, Weidong; Li, Guoqing; Yang, Long

doi:10.1016/j.biocontrol.2016.06.007

Cited by 36 publications

(34 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. yanglinensis 3-10 was originally isolated from a healthy rice leaf grown in the field near Wuhan, China ( Wan et al, 2008 ) and stored at -20°C. It was cultured on fermentation medium (soluble starch 3%, peptone 0.75%, yeast extract 0.025%, soybean meal 1%, K 2 HPO 4 ⋅3H 2 O 0.5 g/l, KH 2 PO 4 0.7 g/l, MgSO 4 ⋅7H 2 O 0.4 g/l, MnSO 4 ⋅H 2 O 0.02 g/l, ZnSO 4 ⋅7H 2 O 0.01 g/l) at 28°C for 72 h for AFS production ( Shakeel et al, 2016 ). A. flavus NRRL 3375 was kindly provided by Dr. Desheng Qi of Huazhong Agricultural University in China and cultured on potato dextrose agar (PDA).…”

Section: Methodsmentioning

confidence: 99%

Biocontrol of Aspergillus flavus on Peanut Kernels Using Streptomyces yanglinensis 3-10

et al. 2018

Self Cite

View full text Add to dashboard Cite

The bacterium, Streptomyces yanglinensis 3-10, shows promise in the control of many phytopathogenic fungi. In this study, S. yanglinensis and its antifungal substances, culture filtrate (CF3-10) and crude extracts (CE3-10), were evaluated for their activity in reducing growth and aflatoxin AFB1 production by Aspergillus flavus, both in vitro and in vivo on peanut kernels. The results showed that in dual culture conditions, S. yanglinensis reduced the mycelial growth of A. flavus about 41% as compared to control. The mycelial growth of A. flavus was completely inhibited on potato dextrose agar amended with CF3-10 at 3% (v/v) or CE3-10 at 2.5 μg/ml. In liquid culture experiments, growth inhibition ranged from 32.3 to 91.9% with reduction in AFB1 production ranging from 46.4 to 93.4% using different concentrations of CF3-10 or CE3-10. For in vivo assays, CF3-10 at 0.133 ml/g (v/w) or CE3-10 at 13.3 μg/g (w/w) reduced the postharvest decay of peanut kernels by inhibiting visible growth of A. flavus leading to an 89.4 or 88.1% reduction in AFB1 detected, respectively. Compared with the controls, CF3-10 and CE3-10 in A. flavus shake culture significantly reduced expression levels of two AFB1 biosynthesis genes, aflR and aflS. Furthermore, electron microscopy observation showed that CF3-10 (2%, v/v) caused hyphae growth to be abnormal and shriveled, cell organelles to degenerate and collapse, large vacuoles to appear. These results suggest that S. yanglinensis 3-10 has potential as an alternative to chemical fungicides in protecting peanut kernels and other agricultural commodities against postharvest decay from A. flavus.

show abstract

Section: Methodsmentioning

confidence: 99%

Biocontrol of Aspergillus flavus on Peanut Kernels Using Streptomyces yanglinensis 3-10

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was isolated from a culture containing spores of S. platensis F-1 jointly treated by UV-irradiation and LiCl (Che, 2011). Aspergillus niger A-1 (a soil saprophyte) was used as indicator in bioassays to detect the antifungal activity of the metabolites of strain 3–10 (Shakeel et al, 2016). The remaining two species of Oomycetes, four species of bacteria, and 21 species of fungi were used to test the antimicrobial spectrum of the metabolites of Streptomyces sp.…”

Section: Methodsmentioning

confidence: 99%

“…The column was eluded with gradient chloroform/methanol solutions (from 99/1 to 0/100, v/v). The resulting fractions were individually assayed for antifungal activity against A. niger using the Oxford cup-agar diffusion method described by Shakeel et al (2016). The fractions that had antifungal activity were combined, concentrated, and loaded in a Sephadex LH-20 chromatography column (GE Healthcare), which was eluded with methanol to remove impurities.…”

Section: Methodsmentioning

confidence: 99%

Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System

et al. 2017

Self Cite

View full text Add to dashboard Cite

This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3–10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3–10 was re-evaluated. The cultural filtrates of strain 3–10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum. Two compounds were purified from the crude extract and were identified as reveromycins A and B, which demonstrated high antifungal activity against Botrytis cinerea, Mucor hiemails, Rhizopus stolonifer, and Sclerotinia sclerotiorum under acidic pH conditions. Both the crude extract and reveromycin A from strain 3–10 at 10, 50, and 100 μg/ml showed high efficacy in suppression of strawberry fruit rot caused by the above-mentioned four pathogens. The efficacy was comparable to that of corresponding commercial fungicides (pyrimethanil, captan, dimetachlone) used in management of these pathogens. Morphological, physiological, and phylogenetic characterization showed that strain 3–10 is closely related to Streptomyces yanglinensis 1307T, representing a novel phylotype in that species. This study reported a new strain with reveromycins-producing capability. The finding is important for further exploitation of reveromycins for agricultural use.

show abstract

“…[30,31]. Secondary metabolites production in actinomycetes is often stimulated by slowly-assirnilated complex carbohydrates such as soluble starch, but is suppressed by rapidly-utilized carbon sources such as presence of glucose [32]. In fact, high concentration usually inhibits the biosynth esis of many antibiotics in Streptomycetes [33][34][35][36].…”

Section: Runsmentioning

confidence: 99%

“…In fact, high concentration usually inhibits the biosynth esis of many antibiotics in Streptomycetes [33][34][35][36]. A possible ex planation of this phenomenon is that glucose may cause catabolic re pression, in which the production of enzymes for secondary metabolites biosynthesis might be inhibited [32,37]. An example of catabolic re pression of secondary metabolism in actinomycetes is that of actino mycin synthesis by Streptomyces antibioticu.s when more glucose is added to the media [37].…”

Section: Runsmentioning

confidence: 99%

Modeling-based optimization approaches for the development of Anti- Agrobacterium tumefaciens activity using Streptomyces sp TN71

Smaoui

Ennouri

Chakchouk-Mtibaa

et al. 2018

Microbial Pathogenesis

View full text Add to dashboard Cite

A new aerobic bacterium TN71 was isolated from Tunisian Saharan soil and has been selected for its antimicrobial activity against phytopathogenic bacteria. Based on cellular morphology, physiological characterization and phylogenetic analysis, this isolate has been assigned as Streptomyces sp. TN71 strain. In an attempt to increase its anti-Agrobacterium tumefaciens activity, GYM + S (glucose, yeast extract, malt extract and starch) medium was selected out of five different production media and the medium composition was optimized. Plackett-Burman design (PBD) was used to select starch, malt extract and glucose as parameters having significant effects on antibacterial activity and a Box-Behnken design was applied for further optimization. The analysis revealed that the optimum concentrations for anti-A. tumefaciens activity of the tested variables were 19.49 g/L for starch, 5.06 g/L for malt extract and 2.07 g/L for glucose. Several Artificial Neural Networks (ANN): the Multilayer perceptron (MLP) and the Radial basis function (RBF) were also constructed to predict anti-A. tumefaciens activity. The comparison between experimental with predicted outputs from ANN and Response Surface Methodology (RSM) were studied. ANN model presents an improvement of 12.36% in terms of determination coefficients of anti A. tumefaciens activity. To our knowledge, this is the first work reporting the statistical versus artificial intelligence based modeling for optimization of bioactive molecules against phytopathogens.

show abstract

Optimization of the cultural medium and conditions for production of antifungal substances by Streptomyces platensis 3-10 and evaluation of its efficacy in suppression of clubroot disease ( Plasmodiophora brassicae ) of oilseed rape

Cited by 36 publications

References 38 publications

Biocontrol of Aspergillus flavus on Peanut Kernels Using Streptomyces yanglinensis 3-10

Biocontrol of Aspergillus flavus on Peanut Kernels Using Streptomyces yanglinensis 3-10

Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System

Modeling-based optimization approaches for the development of Anti- Agrobacterium tumefaciens activity using Streptomyces sp TN71

Contact Info

Product

Resources

About