Optimization of the Cycling of Clonogenic and Primitive Cord Blood Progenitors by Various Growth Factors

Movassagh, Mojgan; Caillot, L.; Baillou, Claude; Guigon, Martine; Lemoine, François M.

doi:10.1002/stem.150214

Cited by 9 publications

(4 citation statements)

References 42 publications

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“…The same results were observed when similar experiments were performed in serum-free conditions, ruling out the possibility that the presence of FBS could have affected the cycling status of the progenitor cells (Cashman et al, 1990;Sitnicka et al, 1995). Our results on the proportion of CFCs and LTC-ICs in the S-phase con®rm those of a recent study by Movassagh et al (1997), who used the [ 3 H]-thymidine incorporation suicide technique and demonstrated that the Ara-C approach is similar to the thymidine suicide approach for the assessment of the proportion of haematopoietic progenitor cells in the S-phase of the cell cycle.…”

Section: Discussionsupporting

confidence: 91%

Cell cycle distribution of cord blood‐derived haematopoietic progenitor cells and their recruitment into the S‐phase of the cell cycle

Lucotti¹,

Malabarba²,

Rosti³

et al. 2000

Br J Haematol

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Summary. The objective of this study was to evaluate the cycling status of cord blood (CB)-derived colony-forming cells (CFC) and long-term culture-initiating cells (LTC-IC), and their recruitment into the S-phase of the cell cycle. By using the cytosine arabinoside (Ara-C) suicide approach, we found that only small proportions of both CFC and LTC-IC were in the S-phase of the cell cycle. These estimates were con®rmed by¯ow cytometric DNA analysis, which showed that 96 6 2% of CB-derived CD34 cells were in G 0 /G 1 and only 1´6 6 0´4% in the S-phase. Staining of CD34 cells with an antistatin monoclonal antibody, a marker of the G 0 phase, indicated that among CD34 cells with a¯ow cytometric DNA content typical of the G 0 /G 1 phase 68 6 7% of cells were in the G 0 phase of the cell cycle. Incubation (24 h) with interleukin 3 (IL-3), recombinant human stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) signi®cantly increased the proportion of cells in the S-phase for both CFC and LTC-IC without inducing any loss in numbers. Flow cytometric DNA analysis also showed an increase in CD34 cells in the S-phase upon continuous exposure to these cytokines. Our ®ndings indicate that: (i) very few CB-derived CFC or LTC-IC were in the S-phase of the cell cycle; (ii) a substantial amount of CD34 cells with ā ow cytometric DNA content typical of the G 0 /G 1 fraction was cycling, as found in the G 1 phase of the cell cycle; and (iii) 24-h incubation with IL-3, SCF and G-CSF could drive a proportion of progenitor cells into the S-phase without reducing their number. These data might be useful for gene transfer protocols and the ex vivo expansion of CB-derived progenitor cells.

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Section: Discussionsupporting

confidence: 91%

Cell cycle distribution of cord blood‐derived haematopoietic progenitor cells and their recruitment into the S‐phase of the cell cycle

Lucotti¹,

Malabarba²,

Rosti³

et al. 2000

Br J Haematol

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“…It is known that granulocyte/macrophage progenitor cells from PB and BM, differ in their response to (for example) prostaglandin E1 (Richman & Johnson 1987). In CB, however, there is high concentration of growth factors and different cytokines, and therefore stem and progenitor cells present in CB are more quiescent (Mavassagh et al . 1997).…”

Section: Discussionmentioning

confidence: 99%

The influence of 3,3′,5‐triiodo‐l‐thyronine on human haematopoiesis

Grymuła¹,

Paczkowska²,

Dziedziejko³

et al. 2007

Cell Proliferation

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“…UCB substantially differs from that of bone marrow or mobilized peripheral blood in quantity, composition, and properties of hematopoietic cells. In contrast to bone marrow HSPCs, UCB HSPCs are outside of the cell cycle, but they have a pronounced and rather fast proliferative response to growth factors stimulation [ 14 - 17 ]. The ability of UCB HSPCs to expand ex vivo in response to stimulation became the basis for the development of different approaches towards increase of the HSPC number in UCB transplants.…”

Section: Introductionmentioning

confidence: 99%

Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood

et al. 2016

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Transplantation of umbilical cord blood cells is currently widely used in modern cell therapy. However, the limited number of hematopoietic stem and progenitor cells (HSPCs) and prolonged time of recovery after the transplantation are significant limitations in the use of cord blood. Ex vivo expansion with various cytokine combinations is one of the most common approaches for increasing the number of HSPCs from one cord blood unit. In addition, there are protocols that enable ex vivo amplification of cord blood cells based on native hematopoietic microenvironmental cues, including stromal components and the tissue-relevant oxygen level. The newest techniques for ex vivo expansion of HSPCs are based on data from the elucidation of the molecular mechanisms governing the hematopoietic niche function. Application of these methods has provided an improvement of several important clinical outcomes. Alternative methods of cord blood transplantation enhancement based on optimization of HPSC homing and engraftment in patient tissues have also been successful. The goal of the present review is to analyze recent methodological approaches to cord blood HSPC ex vivo amplification.

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Optimization of the Cycling of Clonogenic and Primitive Cord Blood Progenitors by Various Growth Factors

Abstract: ABSTRACT

Cited by 9 publications

References 42 publications

Cell cycle distribution of cord blood‐derived haematopoietic progenitor cells and their recruitment into the S‐phase of the cell cycle

Cell cycle distribution of cord blood‐derived haematopoietic progenitor cells and their recruitment into the S‐phase of the cell cycle

The influence of 3,3′,5‐triiodo‐l‐thyronine on human haematopoiesis

Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood

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