Optimization of the efficacy of a SWCNTs-based subunit vaccine against infectious spleen and kidney necrosis virus in mandarin fish

Zhao, Zhao; Xiong, Yan; Zhang, Chen; Jia, Yijun; Qiu, De-Kui; Wang, Gao‐Xue; Zhu, Bin

doi:10.1016/j.fsi.2020.07.062

Cited by 17 publications

(12 citation statements)

References 46 publications

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“…(2016) immunized rainbow trout with B.17-ILM vaccine to prevent bacterial coldwater disease (BCWD), they found that the immunization time was extended from 1.5 min to 30 min, and RPS also increased from 36% to 52%. In addition, similar results appeared in the research of Zhao et al (2020) and Qiu et al (2021). Interestingly, in this study, we found that vaccine immunization for 12 hr achieved an immune protection effect similar to that of immunization for 24 hr, which is discrepancy compare with other study.…”

Section: Discussionsupporting

confidence: 62%

“…However, there have been few reports on the optimization of the programme of immersion immunity. Immunization conditions, such as vaccine dose, immersing immunization time and fish density, have been confirmed in Zhao and Qiu's research in affecting the immune protection effect of the vaccine (Qiu et al, 2021;Zhao et al, 2020).…”

Section: Discussionmentioning

confidence: 86%

“…The experiment process and timeline chart of the present research Currently, vaccine development is mostly focused on the screening of antigen proteins (Zhao et al, 2020), whereas few studies are devoted to the optimization of vaccine dose, immunization time and immunization density during immersion vaccination.…”

Section: Svcv Has Aroused Widespread Interest Among Researchers Inmentioning

confidence: 99%

“…In addition, the immunization time also affected the intake of the vaccine by the fish and thus impressed the immune efficacy of the vaccine (Huising et al, 2003;Tatner, 1987). Study has shown that the immunization time has the most critical impact on the immune response of the immersion vaccine (Zhao et al, 2020); of course, different studies may have different results, but the effect of the immunization time on the immune response of the immersion vaccine is still very important. Therefore, it is extremely important to investigate the influence of vaccine dose, immunization time and immunization density on the immune effect of immersion vaccine.…”

Section: Svcv Has Aroused Widespread Interest Among Researchers Inmentioning

confidence: 99%

“…SWCNTs-MG was prepared based on our previous studies (Qiu et al, 2021;Zhang et al, 2020;Zhao et al, 2020). Briefly, the boric acid buffer containing G protein and the functionalized mannose solution with a concentration of 100 mg/ml were mixed and stirred at room temperature for 24 hr to make them uniformly dispersed.…”

Section: Preparation Of Swcnts-mg Vaccinementioning

confidence: 99%

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Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Gong

Zhang

et al. 2021

Journal of Fish Diseases

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Immersion vaccination of single-walled carbon nanotubes loaded with mannosemodified glycoprotein (SWCNTs-MG) vaccine has been proved to be effective in preventing spring viraemia of carp virus (SVCV). Immunization procedure has immense consequence on the immune effect of the immersion vaccine. However, immunization procedure optimization for SWCNTs-MG vaccine against SVCV has not been reported. In this study, accordingly, a full-factor experiment was designed to optimize the immunization procedure of SWCNTs-MG vaccine by three aspects of vaccine dose (30 mg/L, 40 mg/L and 50 mg/L), immunization density (8 fish L −1 , 24 fish L −1 and 48 fish L −1 ) and immunization time (6, 12 and 24 hr). Furthermore, we used the immunization group (A1B2C1, 30 mg/L, 24 fish L −1 and 6 hr) in the previous study as a positive control (PC) to evaluate the immunization effect optimized conditions from the expression of immune-related genes and relative percentage survival (RPS). At 28 days post-vaccination (DPV), common carps were intraperitoneal injected SVCV challenged test indicated that the A1B2C2 group (30 mg/L, 24 fish L −1 , 12 hr) displayed superiority of protective efficacy compare with other groups and the RPS with 77.9%, which was 15.6% higher than the PC group of RPS with 62.3%.Moreover, the expression of immune-related genes such as IL-10, CD4 and MHC-II was also significantly higher than PC group. The specific experimental flow chart is shown in Figure 1. Conclusively, these results demonstrated that vaccine dose, immunization density and immunization time are 30 mg/L, 24 fish L −1 and 12 hr, which is the more appropriate immunization programme with juvenile carp for SWCNTs-MG vaccine. This study provides a profitable reference for improving the immune efficiency of aquatic immersion vaccine.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 86%

Section: Svcv Has Aroused Widespread Interest Among Researchers Inmentioning

confidence: 99%