Optimization of the Extraction Conditions of Phenolic Compounds From Alchemilla Vulgaris Using Response Surface Methodology

Abstract

The object of this study is to determine the best solvent and optimum extraction conditions for extraction of maximum phenolic compounds from Alchemilla vulgaris leaves. Extractions were carried out using solvents with different polarities. Box-Behnken Design was used to optimize extraction conditions including extraction time, temperature, and liquid/solid ratio. In the study, extract obtained with acetone-water indicated the highest total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity. Optimum extraction conditions for both responses were found as 168 min, 48.5 °C, and liquid/solid ratio of 41:1. The maximum TPC and TFC values were determined as 7.17 mg GAE/gdw and 3.63 mg QE/gdw under optimum extraction conditions. Concentrations of five phenolic compounds analyzed by HPLC increased significantl after optimization. The results indicated that optimizing extraction conditions is critical for quantification of phenolic compounds. The present model can contribute to food industry where phenolic compounds have potential use as biopreservatives.