Optimization of the extraction of orange peel pectin and evaluation of its antiproliferative activity towards HEp2 cancer cells

Pectin a multifaceted biopolymer in the management of cancer: A review

“…Purified pectin (57.7 % homogalacturonan and 42.0 % rhamnogalacturonan-I, with long neutral side chains) isolated from Campomanesia xanthocarpa Berg, a Brazilian Myrtaceae family species, showed cytotoxic effect on glioblastoma cells, with associated increase in the cellular reactive oxygen species (ROS) levels (48 h treatment) and no cytotoxicity on normal NIH 3T3 cells [ 30 ]. Chemically modified and irradiated orange peel pectin showed cytotoxic activity on HepG2 [ 31 ]. It was found that heat-modified citrus pectin contained 4,5-dihydroxy-2-cyclopenten-1-one which showed cytotoxicity against HepG2 by forming covalent adducts on cysteines in tubulin, thereby preventing microtubule formation and cell cycle [ 32 ].…”

“… NR [ 90 ] Orange peel De-esterified (alkaline hydrolysis) and irradiated (25 kGy) pectin Human epidermoid laryngeal cancer cells (HEp2) and normal African green monkey kidney cells [ 91 ] Mortality rate of HEp2 cells reached 95 % when treated with pectin at a concentration of 5 mg/mL, the same concentration exhibited lower mortality rate (41 %) with VERO cells. NR [ 31 ] Potato Rhamnogalacturonan I Molecular weight: 42 kDa Human colon cancer cell line (DLD1) Dose- and time-dependent reduction in the proliferation of DLD1 cells, induced the detachment of cells, reduced expression of the adhesion molecule ICAM1. Homogalacturonan segments in rhamnogalacturonan I and neutral sugar side- chains were essential for bioactivity.…”