Optimization of the nanocellulose based cryoprotective medium to enhance the viability of freeze dried Lactobacillus plantarum using response surface methodology

Survival and stability of Lactobacillus fermentum and Wickerhamomyces anomalus strains upon lyophilisation with different cryoprotectant agents

“…The addition of 10% and 15% of trehalose before lyophilisation also demonstrated a cryoprotective effect directly proportional to the added concentration of trehalose in microorganism viability in the preparation of a lyophilized sourdough (Stefanello et al, 2018a). The survivability of L. plantarum cells after freeze-drying also increased as the concentration of trehalose rose from 0% to 30% (Keivani Nahr et al, 2015) and this could be an option to improve the viability rates when trehalose was the protectant agent of the microrganism evaluated in this study (L. fermentum and W. anomalus), however, due to economic reasons higher amounts were not tested in our study. The main objective of our study was to find good and relatively cheap agents allowing for commercially viable production of starters of W. anomalus IAL 4533 and L. fermentum IAL 4541, and skin milk powder, as well glutamate, are cheaper and more economically advantageous than the use of trehalose.…”

“…The combination of trehalose, sucrose and skim milk was the most effective protection and gave a survival rate between 83% and 85% after freeze-drying, however the stability along the storage cannot be compered, since in that study samples were maintained at −85 °C (Zayed & Roos, 2004). An average concentration of skim milk and a high concentration of trehalose also improved tolerance to freezing followed by the drying of L. plantarum (Keivani Nahr et al, 2015).…”

“…After 12 h of freezing, cultures were transferred to a freeze-dryer (K105, Liotop, Brazil). Freeze-drying was carried out for 24 h at −50 °C and at a pressure of 0.15 mbar (Keivani Nahr et al, 2015). After closing, the vials containing lyophilized strains were sealed with Parafilm® and stored protected from light at room temperature for 1 year.…”

Formulation and Safety Tests of a Wickerhamomyces anomalus–Based Product: Potential Use of Killer Toxins of a Mosquito Symbiotic Yeast to Limit Malaria Transmission

“…The samples were immediately frozen (at −80 • C) for 12 h; then, the cultures were transferred to a freeze-dryer (Edwards, Burgess Hill, United Kingdom). Freeze-drying was carried out for 24 h at −50 • C and at a pressure of 0.15 mbar [33]. The vials containing lyophilized strains were sealed with Parafilm (Darmstadt, Germany) and stored up to 60 days at rt and protected from light.…”

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

“…A composition must be found to create a suitable powder lyophilizate, making it possible to obtain a homogeneous material for encapsulation while allowing the bacteria to survive in the required time interval. Insoluble silicates [ 42 ] and cellulose derivates [ 43 ], used as constitutional excipients, and water-soluble polyols [ 44 ] or saccharides [ 45 ], which primarily act as cryoprotectants, could be promising for the formation of the required lyophilizates [ 46 ]. Hence, this work deals with the formulation and evaluation of a total of 16 lyophilizate compositions containing Lactobacillus plantarum R2 in mixtures with magnesium aluminosilicates (Neusilin ® US2 (US2); Neusilin ® NS2N (NS2N)), microcrystalline cellulose (Avicel ® PH-101 (AV)), and saccharides (inulin (IN), saccharose (SA), and modified starch 1500 ® (ST)).…”