Optimization of the separation lactic acid enantiomers in body fluids by capillary electrophoresis

How experimental design can improve the validation process. Studies in pharmaceutical analysis

“…In fact, a method can become specific/selective thanks to the optimisation of the experimental conditions. This is a simple example where optimisation and validation are linked together and several experimental design applications for this purpose can be found in the literature [24,36,37,38,39,40,41,42]. It should be noted that most of the cited works commonly deal with optimising resolution, rather than method selectivity.…”

Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

“…Selected papers which were not mentioned before are also included. On the other hand, work dealing with the CE monitoring of the enantiomers of amino acids and acids in body fluids and tissues, including aspartic acid [46], tryptophan [47] and lactic acid [48], is not included in this review. As with our previous compilation 10 years ago [26], insight into the achievements is presented via distinction between enantiomer analysis in fortified body fluids (Table 1), body fluids and tissues of humans and animals (Tables 2 and 3), in vitro preparations (Table 4) and in forensic biosamples (Table 5 [ ).…”

Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: An update

“…Further biological applications include the determination of lactic acid enantiomers in body fluids such as urine, amniotic fluid and cerebrospinal fluid [118]. Utilizing factorial design, an assay was developed in a polyacrylamide-coated capillary utilizing a pH 6.0 phosphate buffer containing HP-b-CD as chiral selector.…”