Optimization of the separation of phenolic compounds by micellar electrokinetic capillary chromatography

Rodríguez-Delgado, M. A.; Perez, Martine; Corbella, R.; González, G.; Montelongo, F. J. García

doi:10.1016/s0021-9673(99)00883-3

Cited by 57 publications

(35 citation statements)

References 26 publications

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“…The detection limit (3σ) was 9.6 × 10 -8 mol l -1 , which was similar, or an order of magnitude lower than, to those previously reported by other methods. [11][12][13][14][15] The relative standard deviation was 1.0% for 11 replicate analyses of 2.0 × 10 -4 mol l -1 ferulic acid. A typical recording output of the proposed CL system for the determination of different concentrations of ferulic acid is shown in Fig.…”

Section: Analytical Characteristicsmentioning

confidence: 91%

“…In recent years, the TLC and HPLC techniques have been developed and commonly employed for the determination of ferulic acid. [7][8][9][10][11][12] Some new methods have been proposed, such as micellar electrokinetic capillary chromatography, 13 micellar electrokinetic chromatography, 14 liquid chromatography-mass spectrometry, 15 and high-performance capillary electrophoresis. 16 Each of these proposed methods often offer their own set of advantages and disadvantages.…”

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confidence: 99%

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Permanganate-based Chemiluminescence Analysis of Ferulic Acid Using Flow Injection

Li¹,

Li²,

Luo³

2005

ANAL. SCI.

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Section: Analytical Characteristicsmentioning

confidence: 91%

mentioning

confidence: 99%

Permanganate-based Chemiluminescence Analysis of Ferulic Acid Using Flow Injection

Li¹,

Li²,

Luo³

2005

ANAL. SCI.

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“…Therefore, a sample-preparation procedure is a necessary step prior to the electrophoretic analysis, in order to isolate the analytes under study from real samples. Different preconcentration methods have been used over the years, including solid-phase extraction (SPE) with C-18, silica, or other cartridges [14,16,19] and liquid-liquid extraction (LLE) with different organic solvents [10,20,21].…”

Section: Applicationmentioning

confidence: 99%

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Kapnissi‐Christodoulou¹

2012

Electrophoresis

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In addition, hydrogen bonding, dipole-dipole, and dispersive interactions can contribute to the solute partitioning between the two phases [4][5][6].CE was originally considered as a powerful analytical tool for the analysis of biological macromolecules. It has though, over the years, been extensively used for the separation of other compounds, such as chiral drugs, food additives, pesticides, inorganic ions, organic acids, and others. In this chapter, the ability of CE, and particularly CZE and MEKC, to be used for the qualitative and quantitative determination of compounds in pharmaceutical, biological and natural samples is investigated. In each approach, a number of studies are reported and discussed. These studies involve establishment of optimum separation conditions, method validation, optimization of sample-preparation procedure and application for the determination of the analytes under study in real samples. The first part of this chapter involves the determination of polyphenolic compounds using CZE with UVVis detector in red and white wines, while the second part involves the determination of pharmaceutical compounds in biological samples, such as blood and urine, using the hyphenated technique CE-MS (mass spectrometry). The third and final part emphasizes the importance of MEKC in chiral analysis since it has been known that usually only one enantiomer is active, while the other may be less active, inactive or has adverse effects. Determination of polyphenolic compounds in natural samplesPolyphenolic compounds exist in a variety of natural products, such as fruits, vegetables, beverages (tea, wine and juices), honey, cacao and herbs. They attract a lot of interest due to their beneficial implication in human health. They have been widely studied due to their antioxidant capacity and their association with several pathological conditions, such as hypertension, cardiovascular disease, dementia, and even cancer [7][8][9]. Therefore, due to their health significance, numerous analytical methods have, over the last decades, been developed for their separation, identification and quantitation in natural products [10][11][12][13].According to literature, the simplest CE method, CZE, proved to be the best method for the determination of polyphenolic compounds in wine samples [14][15][16][17]. In such studies, and in each case, when the optimum CZE method was applied to different red and white wines, it was established that red wines have higher levels of polyphenolic compounds than white wines and that the polyphenolic composition varies among different wines.

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“…Capillary electrophoresis (CE) has been shown to be a fast, powerful, clean and efficient separation technique for a wide variety of compounds (1)(2)(3)(4). Up to now, many detection methods coupled with CE involving the absorbance spectrometry, fluorescence, mass spectrum, conductivity and electrochemistry are commonly used.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence determination of pharmacologically active compounds by capillary electrophoresis

Han

2005

Luminescence

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A simple and rapid capillary electrophoresis with direct chemiluminescence method has been developed for the determination of five natural pharmacologically active compounds including rutin, protocatechuic aldehyde, chlorogenic acid, luteolin and protocatechuic acid. The luminol as a component of the separation electrolyte buffer was introduced at the head of the separation capillary. The separation of five compounds was carried out in a fused-silica capillary with 15.0 mmol/L tetraborate, 1.0 mmol/L SDS and 0.42 mmol/L luminol (pH 8.5). The analytes was determined by enhancing the chemiluminescence of luminol with 0.13 mmol/L K3Fe(CN)6 in 0.05 mol/L NaOH, which was introduced at the post-column stage. The voltage applied was 16 kV. Under the optimum conditions, the analytes were separated within 10 min. The excellent linearity was obtained over two to three orders of magnitude with a detection limit (signal:noise = 3) of 0.012-0.055 micromol/L for all five analytes. The method was successfully used in the analysis of pharmaceutical and biological samples, and the assay results were satisfactory.

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Optimization of the separation of phenolic compounds by micellar electrokinetic capillary chromatography

Cited by 57 publications

References 26 publications

Permanganate-based Chemiluminescence Analysis of Ferulic Acid Using Flow Injection

Permanganate-based Chemiluminescence Analysis of Ferulic Acid Using Flow Injection

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Chemiluminescence determination of pharmacologically active compounds by capillary electrophoresis

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