Optimization of the simultaneous production of cellulase and xylanase by submerged and solid-state fermentation of wheat chaff

Abstract

Wheat chaff as an agricultural waste represents a cheap raw material for biotechnological processes. With its lignocellulosic composition, it is suitable for producing hydrolytic enzymes for second generation renewable fuel production technologies. The aim of this work was to optimize the process parameters (cultivation temperature 25-35 °C, pH value 4-6 and cultivation time 3-7 days) of the cultivating fungi (Trichoderma reesei QM 9414) on a media based on wheat chaff by submerged and solid-state techniques, in order to enhance and compare the two types of simultaneous cellulase and xylanase production. Optimal conditions for the submerged fermentation were 29.65 °C for temperature, pH 4.27 and 7 days of cultivation, while for the solid-state fermentation, the optimal conditions were 28.01 °C, pH 6.00 and 7 days. The cellulolytic and xylanolytic activities of the obtained cultivation broth filtrates were 0.0535 and 0.1676 U mL-1 for the submerged fermentation, and 0.0407 and 0.1401 U mL-1 for the solid-state fermentation, respectively, and with a 26.77 and 13.39 % enhancement of enzyme activity for submerged fermentation, and a 22.96 and 42.66 % enhancement for solid-state fermentation, respectively, compared to the results obtained before optimization.

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

“…Similar findings were reported for Aspergillus niger [72]. Maximum production was reported at 29 • C for Trichoderma reesei QM 9414 [73]. Bacillus subtilis and B. circulans produced minimum cellulase at 45 • C, whereas the maximum production was obtained at 40 • C [74].…”

Biotechnological Utilization of Agro-Industrial Residues and By-Products—Sustainable Production of Biosurfactants

“…Different agro-industrial residues, wastes, and by-products are used for the production of different bioproducts. For example, soybean molasses has been used for the production of bioethanol [ 11 ], biogas [ 12 ], and extracellular polymers [ 13 ]; sugar beet molasses for single cell proteins [ 14 ], bioethanol [ 15 , 16 ], and biohydrogen [ 17 ]; sugar beet pulp for single cell proteins [ 14 ], and bioethanol, biomethane, and biohydrogen [ 18 ]; wheat chaff for cellulase and xylanase [ 19 ], and itaconic acid [ 20 ]; corn straw for bioethanol and biogas [ 21 ], fumaric acid [ 22 ], and lactic acid [ 23 ]; and wheat straw for lactic acid [ 24 ], bioethanol, biohydrogen, and biogas [ 25 ]; while wastewaters obtained in the different industrial processes were used for the production of lipases, proteases, and tannases [ 26 ], xanthan [ 27 , 28 ], bioethanol [ 29 ], and biomass [ 30 ], etc.…”