Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method

Song, Huibo; Zhao, Genhai; Zhang, Ming; Bi, Ruiming; Xiao, Meng; Song, Jian; Wang, Bo; Liu, Jian; Liu, Li; Lyu, Yongmei; Zhang, Xiaoyang

doi:10.1016/j.pep.2021.106002

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…The significance was determined by p -value in variance analysis and R -value in range analysis. Only the factor of the liquid–solid ratio had an extremely significant influence on the content of the WHSs and the nitrogen loss . In the papermaking, the liquid-to-wood ratio is a decisive factor for the energy efficiency in the sulfite-based pretreatment, which is consistent with the finding of this study .…”

Section: Resultssupporting

confidence: 91%

“…Only the factor of the liquid−solid ratio had an extremely significant influence on the content of the WHSs and the nitrogen loss. 24 In the papermaking, the liquid-to-wood ratio is a decisive factor for the energy efficiency in the sulfite-based pretreatment, which is consistent with the finding of this study. 25 The results suggest that the liquid−solid ratio of 4:1 was the optimum option in the study, and the reaction temperature and reaction time only had a mild effect on ASS activation.…”

Section: Optimal Activation Routesupporting

confidence: 91%

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Ammonium Sulfite Slurry from Ammonia-Based Desulfurization Activates Water-Soluble Humic Substances from Lignite: Performance, Application, and Mechanism

Zhao,

Chen,

Yao

et al. 2023

J. Agric. Food Chem.

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Both ammonium sulfite slurry (ASS) from ammonia-based desulfurization and lignite are waste materials with low value. In this work, an innovative method was developed by applying ASS in lignite activation to produce water-soluble humic substances (WHSs) with a high bioactivity and economic value. The optimal activation method was to mix lignite and ASS at a 4:1liquid−solid ratio by vortex blender and then oscillate it for 30 min at 25 °C. Compared with that of the unactivated lignite (UAL), the yield of WHSs from activated lignite (AL) increased by 42.72%. WHSs from AL consisted of a large number of aliphatic carbons with low molecular weight and functional groups such as amides, amines, sulfonic acid groups, C−O, and so forth. Moreover, WHSs from AL at lower concentrations (2 mg/L) has a more obvious root-elongation-promoting effect than WHSs from UAL (10 mg/L). Activation experiment with the lignite-related model compounds revealed that ASS caused the breakage of C aliph −O, C aliph −C aliph , and C arom −C aliph linkages between aromatic rings. These findings provide a theoretical basis for the development of green and sustainable technologies for the beneficial reuse of ASS and lignite in agriculture.

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Section: Resultssupporting

confidence: 91%

Section: Optimal Activation Routesupporting

confidence: 91%

Ammonium Sulfite Slurry from Ammonia-Based Desulfurization Activates Water-Soluble Humic Substances from Lignite: Performance, Application, and Mechanism

Zhao,

Chen,

Yao

et al. 2023

J. Agric. Food Chem.

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“…The GlcN yield was a little higher than the highest value in the orthogonal test. A combination with the application of orthogonal design on the biosynthesis of other compounds [59] indicated that the orthogonal array for the optimization of GlcN biotransformation was available. The yield of GlcN hydrolyzed from shrimp shell increased 20.09% with the optimized condition.…”

Section: Optimization Of Glcn Biosynthesis By Orthogonal Designmentioning

confidence: 99%

Heterologous Expression of a Thermostable Chitinase from Myxococcus xanthus and Its Application for High Yield Production of Glucosamine from Shrimp Shell

Lyu

Zheng

Qiu

et al. 2021

Foods

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Glucosamine (GlcN) is a widely used food supplement. Hence, enormous attention has been concerned with enzymatic production of GlcN owing to its advantage over a chemical approach. In this study, a previously unstudied chitinase gene (MxChi) in the genome of Myxococcus xanthus was cloned, expressed in recombinant soluble form and purified to homogeneity. TLC-, UPLC-, and microplate-reader- based activity tests confirmed MxChi hydrolyzes colloidal chitin to chitobiose as sole product. The optimal catalytic pH and temperature of MxChi was identified as 7.0 and 55 °C, respectively. MxChi exhibited 80% activity after 72 h incubation at 37 °C. The site-directed mutagenesis revealed that the amino acids D323A, D325A, and E327A of MxChi were in the DXDXE catalytic motif of GH18. When coupled with β-N-acetylhexosaminidase (SnHex) and deacetylase (CmCBDA), the enzyme allowed one-pot extraction of GlcN from colloidal chitin and shrimp shell. The optimal condition was 37 °C, pH 8.0, and 1/3/16.5 (MxChi/SnHex/CmCBDA), conducted by orthogonal design for the enzymatic cascades. Under this condition, the yield of GlcN was 26.33 mg from 400 mg shrimp shell. Facile recombinant in E. coli, robust thermostability and pure product herein makes newly discovered chitinase a valuable candidate for the green recycling of chitin rich waste.

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Study on jet formation behavior and optimization of trunconical hypercumulation shaped charge structure

Qu²,

Wang³

et al. 2023

Defence Technology

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Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method

Cited by 6 publications

References 45 publications

Ammonium Sulfite Slurry from Ammonia-Based Desulfurization Activates Water-Soluble Humic Substances from Lignite: Performance, Application, and Mechanism

Ammonium Sulfite Slurry from Ammonia-Based Desulfurization Activates Water-Soluble Humic Substances from Lignite: Performance, Application, and Mechanism

Heterologous Expression of a Thermostable Chitinase from Myxococcus xanthus and Its Application for High Yield Production of Glucosamine from Shrimp Shell

Study on jet formation behavior and optimization of trunconical hypercumulation shaped charge structure

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