Optimization of Tissue Culture Protocol for Regeneration and Direct Multiplication of Moringa Oleifera by Seed Germination.

Chaudhary, Asfandyar; Ali, Kashif; Farid, Neha; Maqsood, Nosheen

doi:10.21474/ijar01/8851

Cited by 2 publications

(11 citation statements)

References 17 publications

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“…The data in Table (1) and Figure (1) showed that all concentrations of BA and most concentrations of Kin added to the MS medium achieved significant differences in the multiplication of single nodes compared with the control treatment. While the largest number of shoots reached 17.4 shoots/nodule, resulting from the nodes cultured in the nutrient media equipped with 0.75 mg.l -1 BA.…”

Section: Number Of Shootsmentioning

confidence: 97%

“…Table (2) and Figure (1) show that the addition of BA to the MS medium caused a significant decrease in the lengths of the shoots resulting from the culture of nodes after eight weeks. The highest mean of the lengths of the shoots was obtained from the comparison treatment, which was 5.0 cm.…”

Section: Lengths Of the Shootsmentioning

confidence: 98%

“…It is a deciduous perennial tree that falls within forest trees or ornamental trees. Moringa trees are drought tolerant so they can grow in unsuitable conditions [1].Moringa tree has several common names, including Drumstick, Horseradish, and Miracle Tree, as it provides a rich food source for the poor [2]. Moringa trees have many economic and medicinal benefits, as their seeds are eaten while they are green or after boiling them as tea, in addition to using them to purify water.…”

Section: Introductionmentioning

confidence: 99%

“…Moringa trees have many economic and medicinal benefits, as their seeds are eaten while they are green or after boiling them as tea, in addition to using them to purify water. As for their leaves, they are very rich in nutrients, vitamins, and minerals and work to increase milk production [1],as they contain vitamin A more than carrots, more vitamin C than oranges, more calcium than milk, more of iron than spinach, more of potassium than bananas, and a protein quality that rivals that of milk and eggs [3]. On the other hand, [4] mentioned that the fruits, flowers, and leaves of Moringa trees contain (5-10%) protein.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to what was mentioned above, Moringa trees have high anti-tumor efficacy and play an important role in protecting the liver. This is what encouraged workers in the agricultural and pharmaceutical fields to pay attention to it, study it, and spread its culture more widely worldwide [1]. Moringa trees are traditionally propagated by seeds or cuttings, but each of them has its problems.…”

Section: Introductionmentioning

confidence: 99%

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The Response of Moringa oleifera Lam. Nodes to Multiply on MS and WPM Media Supplemented with Different Concentrations of BA and Kin

Al-Hamidi,

Bashi,

Hadedy

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The study was conducted at the laboratory of Plant Cells and Tissue Culture/Department of Forestry Sciences in the College of Agriculture and Forestry/the University of Mosul, during the year 2021-2022 AD on Moringa oleifera Lam. to spread the In vitro propagation. The effect of the type of nutrient medium (MS and WPM) and the type of cytokinin 6-Benzyladenine (BA) at the concentrations (0, 0.25, 0.5, 0.75, 1) mg.l−1 and Kinetin (Kin) at concentrations (0, 1, 2, 3, 4) mg.l−1 In the growth and multiplication of single nodes prepared from seedlings at 21 day. After eight weeks of cultivation, the results showed that there was a significant effect of the type of nutrient medium on the growth and multiplication of single nodes, as the MS medium was superior to the WPM medium in the number of shoots and leaves per single node. The best result was (6.4 and 4.7 shoot/node and 34.5 and 27.3 leaf/node) on the leaves, respectively, BA was also significantly superior to Kin in the number of shoots and the number of leaves per node which achieved (10.4 and 2.7 shoots/node and 52.1 and 19.5 leaves/node), respectively. On the other hand, the interaction (0.75 mg.l−1 BA with 4 mg.l−1Kin) in MS media caused the highest rate of the number of shoots and number of leaves achieved (19.6 shoots/node and 82.9 leaves/node), respectively.

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Section: Number Of Shootsmentioning

confidence: 97%

Section: Lengths Of the Shootsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Response of Moringa oleifera Lam. Nodes to Multiply on MS and WPM Media Supplemented with Different Concentrations of BA and Kin

Al-Hamidi,

Bashi,

Hadedy

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Effect of 2,4-D and NAA in Callus Induction and Differentiation from Different Explants of Moringa Oleifera Lam

Al-Hamidi,

Al-Hadedy,

Bashi

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study was carry out in the Laboratory of Plant Cells and Tissue Culture / Department of Forestry Sciences in the College of Agriculture and Forestry / University of Mosul during the year 2021-2022 to study the effect of adding different concentrations of 2,4-Dichloro-Phenoxyacetic acid (2,4-D) or Naphthalene Acetic Acid (NAA) to the MS medium in the induction of callus from different parts of the Moringa oleifera Lam. and then study the effect of different concentrations of 6-Benzyladenine (BA) and Kinetin (Kin) on the differentiation of callus into buds and embryogenesis. Concerning callus induction, the results showed that 2,4-D or NAA in the nutrient medium was necessary after three weeks of cultivation 2,4-D was superior to NAA in the percentage of callus induction, as callus induction occurred by 100% of the explants cultured in the media supplemented with 0.1 mg.l−1 2,4-D. On the other hand, the parts (apical buds, single node, parts of the leaf blade, epicotyl, cotyledon, hypocotyl) differed significantly among themselves in the percentage of their ability on callus induction, as the cotyledons gave the highest rate of induction, amounting to 80%. The shoots resulting from the callus rooted after four weeks of cultivation in media supplemented with different concentrations of IBA at a rate of 100%, regardless of the concentration of IBA. The study proved the existence of a genetic match between the mother plants and the plants produced from the callus induction from them.

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Optimization of Tissue Culture Protocol for Regeneration and Direct Multiplication of Moringa Oleifera by Seed Germination.

Cited by 2 publications

References 17 publications

The Response of Moringa oleifera Lam. Nodes to Multiply on MS and WPM Media Supplemented with Different Concentrations of BA and Kin

The Response of Moringa oleifera Lam. Nodes to Multiply on MS and WPM Media Supplemented with Different Concentrations of BA and Kin

Effect of 2,4-D and NAA in Callus Induction and Differentiation from Different Explants of Moringa Oleifera Lam

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