Optimization of Total Flavonoids Extraction from Coreopsis tinctoria Nutt. by Response Surface Methodology

Abstract

Response surface methodology (RSM) was applied to predict optimum conditions for extraction of flavonoid from Coreopsis tinctoria Nutt. A central composite design (CCD) was used to monitor the effect of extraction temperature, extraction time, and water-to-material ratio on yield of total flavonoids. The optimal extraction conditions were obtained as water-to-material ratio of 55 ml g −1 , extraction temperature of 80 °C and extraction time of 70 minutes. Under these conditions, the average total flavonoids yield, according to the mass of raw material, was 9.0 ± 0.6 %, which corresponds to the predicted value of 8.9 %. Thus, the extraction method was applied successfully to extract total flavonoids from C. tinctoria.

Synergistic Interactions between Christia vespertilionis Leaves Extract and Chemotherapy Drug Cyclophosphamide on WRL-68 Cell Line

“…[110] CODEN (USA): AJPRHS diffusion rate during extraction, high temperature also causes degradation of phenolic compounds 22 . In addition, flavonoids are also heat-sensitive and high extraction temperature lead to compound degradation 23 . HPLC profiling of both extracts as well as compound identification were conducted and chromatograms are shown in Figure 2.…”

Optimization of Extraction Conditions for Total Phenolics and Total Flavonoids from <i>Kaempferia parviflora</i> Rhizomes

“…In a study carried out by Liu et al [33], with a fixed water-to-raw-material ra- shown that the application of very high temperatures (≥95˚C) may also alter the concentration and composition of phenolic compounds [38]. Although some researchers hold the view that the critical temperature for flavonoid extraction is below 80˚C [39], this threshold temperature would be variable in different plants and plant organs, with PAL or CHS enzymes activity mediating the effects [40].…”

Enzyme-treated Wheat Straw-based PVOH Bio-composites: Development and Characterization

“…Beyond an optimum range, the increase in the concentration of plasticizer led to remarkable results in the mechanical strength of the composites (Liu et al 2014). The use of a plasticizer resulted in the conformational changes, via altered interactions between the host polymers to the reinforcement fibre (Kfoury et al 2013), and thus contributed to increased mobility.…”