Optimization of Trehalase in Aspergillus niger

Mahmood, Akhtar

doi:10.18782/2320-7051.4030

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…spores during early germination and ensures that spores are supplied with the necessary intermediates and energy (Thevelein et al, 1984;Chrungu et al, 2017). Due to the degradation of trehalose into higher concentrations of smaller molecules, the osmotic pressure increases until the filament begins its emergence and facilitates the germination processes (Undeen, 1990).…”

Section: Discussionmentioning

confidence: 99%

Spore Germination of the Obligate Biotroph Spongospora subterranea: Transcriptome Analysis Reveals Germination Associated Genes

et al. 2021

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For soilborne pathogens, germination of the resting or dormant propagule that enables persistence within the soil environment is a key point in pathogenesis. Spongospora subterranea is an obligate soilborne protozoan that infects the roots and tubers of potato causing root and powdery scab disease for which there are currently no effective controls. A better understanding of the molecular basis of resting spore germination of S. subterranea could be important for development of novel disease interventions. However, as an obligate biotroph and soil dwelling organism, the application of new omics techniques for the study of the pre-infection process in S. subterranea has been problematic. Here, RNA sequencing was used to analyse the reprogramming of S. subterranea resting spores during the transition to zoospores in an in-vitro model. More than 63 million mean high-quality reads per sample were generated from the resting and germinating spores. By using a combination of reference-based and de novo transcriptome assembly, 6,664 unigenes were identified. The identified unigenes were subsequently annotated based on known proteins using BLAST search. Of 5,448 annotated genes, 570 genes were identified to be differentially expressed during the germination of S. subterranea resting spores, with most of the significant genes belonging to transcription and translation, amino acids biosynthesis, transport, energy metabolic processes, fatty acid metabolism, stress response and DNA repair. The datasets generated in this study provide a basic knowledge of the physiological processes associated with spore germination and will facilitate functional predictions of novel genes in S. subterranea and other plasmodiophorids. We introduce several candidate genes related to the germination of an obligate biotrophic soilborne pathogen which could be applied to the development of antimicrobial agents for soil inoculum management.

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Section: Discussionmentioning

confidence: 99%

Spore Germination of the Obligate Biotroph Spongospora subterranea: Transcriptome Analysis Reveals Germination Associated Genes

et al. 2021

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Effects of destruxin A on the trehalose content, trehalase activity and their gene expression ofBemisia tabaci

Zhang¹,

Qiu

2023

Preprint

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Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a serious pest of agricultural crops in different regions of the world. Destruxin A (DA) is one of 39 derivatives of destruxins, which are secondary metabolites produced by entomopathogenic fungi. Due to the high insecticidal activity, DA have great application potential in biological control of B. tabaci. In order to understand the impact of destruxin A on the energy substances of the global pest B. tabaci, we measured the changes in energy substance content of the B. tabaci adults treated with DA at LC10 and LC50 concentrations after 4, 8, and 12 hours. The results revealed that DA caused varying degrees of decline in trehalose, soluble sugars, and glycogen content, indicating its ability to influence the energy substance content of whiteflies. After DA treatment, the content of trehalose in B. tabaci decreased significantly, while the activity of trehalase, a specific hydrolase for trehalose, increased significantly. Further study of the expression levels of two genes (BtTre-1 and BtTre-2) encoding trehalases in B. tabaci after DA treatment showed that the soluble trehalase gene, BtTre-1, was induced in both low and high concentrations of DA, while the membrane-bound trehalase gene, BtTre-2, did not show significant changes in expression level.

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Constitutional Fundamentals

Aroney¹

2018

SSRN Journal

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Optimization of Trehalase in Aspergillus niger

Cited by 3 publications

References 17 publications

Spore Germination of the Obligate Biotroph Spongospora subterranea: Transcriptome Analysis Reveals Germination Associated Genes

Spore Germination of the Obligate Biotroph Spongospora subterranea: Transcriptome Analysis Reveals Germination Associated Genes

Effects of destruxin A on the trehalose content, trehalase activity and their gene expression ofBemisia tabaci

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