Optimization of ultrasonic-assisted deep eutectic solvent for the extraction of polysaccharides from Indocalamus tessellatus leaves and their biological studies

Zhang, Yalan; He, Li; Li, Qin; Cheng, Junwen; Wang, Yanbin; Zhao, Jiancheng; Yuan, Shaofei; Chen, Yongjian; Shi, Rui

doi:10.1016/j.scp.2022.100855

Cited by 20 publications

(17 citation statements)

References 60 publications

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“…The FT-IR spectra of all neutral and acid polysaccharide components were similar, displaying typical polysaccharide absorption peaks. The peaks at 3432 cm -1 and 2927 cm -1 are attributed to O-H and C-H stretching vibrations, respectively [24]; the absorption peak near 1516 cm -1 1635 cm -1 is caused by asymmetric and symmetric stretching vibration of C=O [25]; the absorption peak at 1457cm -1 is caused by the bending vibration of C-H; the peak at 1396 cm -1 is attributed to the stretching vibration of C=O on COOH, which proves that all the three components contain uronic acid structure [26]; the peak for BFP-3…”

Section: Ft-ir Analysis Of Bfpmentioning

confidence: 83%

Structural Characterization of Blueberry Fruit Polysaccharides and Assessment of its Hypolipidemic and Immunological Activity

Wang,

Qin,

Cui

et al. 2024

Food Science and Human Wellness

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In this study, polysaccharides were extracted from blueberry fruit (BFP) and isolated to three components (BFP-1, BFP-2 and BFP-3). The molecular weight, monosaccharide composition, characteristic groups, microscopic morphology, and triple helical conformation of the polysaccharides were characterized using HPGPC, HPLC, GC-MS, FT-IR, SEM and Congo red staining. Moreover, the hypolipidemic and immunological activities of the polysaccharides were also assessed. Results showed that the molecular weights of polysaccharides BFP-1, BFP-2, and BFP-3 were 5.547  10 4 , 5.671  10 4 , and 3.951  10 4 Da, respectively, the main monosaccharides were Glc, Gal and Ara,but BFP-3 was mainly composed of GalA, Glc, Gal, and Ara. The backbone of BFP-1 was →4)-Glcp-(1→, which branches to Ara and Xyl residues, while the backbone of BFP-2 was →5)-Araf-(1→, which branches to Xyl, Glu, Rha and Gal residues, in particularly, BFP-3 has a more complex branching with a →3,6)-Galp-(1→) backbone, The side chain is dominated by Araf-(1→). Blueberry polysaccharides are pyran-type polysaccharides with α-glycosidic bonds, and BFP-1 has a typical triple-helical structure. The activity assay revealed that the binding of BFP-3 to sodium glycylcholate hydrate and sodium taurocholate was 79.95% and 78.5%, respectively, indicating that it had better hypolipidemic activity than the others. Immunoactivity assay showed that BFP promoted NO secretion through activating the NF-κB signalling pathway in RAW264.7 cells, which played a role in enhancing the immune function of the organism. These findings may provide a reference for the development and application of blueberry polysaccharides in functional food and medicine.

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Section: Ft-ir Analysis Of Bfpmentioning

confidence: 83%

Structural Characterization of Blueberry Fruit Polysaccharides and Assessment of its Hypolipidemic and Immunological Activity

Wang,

Qin,

Cui

et al. 2024

Food Science and Human Wellness

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“…In the fingerprint region, prominent absorption peaks were observed at 1268.9 cm −1 , 1140.2 cm −1 , 1002.7 cm −1 , 899.8 cm −1 , 831.7 cm −1 , 1278.6 cm −1 , 1111.7 cm −1 , 1006.5 cm −1 , 912.1 cm −1 , 838.8 cm −1 . The multiple peaks were generated by the stretching vibration of single bonds [38] . The characteristic absorption peaks at 3395.1 cm −1 and 3407.4 cm −1 indicating the presence of intermolecular hydrogen bonds in ICPC-b and UICPC-b [39] .…”

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound-assisted extraction of Imperata cylindrica polysaccharides and evaluation of its anti-oxidant and amelioration of uric acid stimulated cell apoptosis

Yu,

Li,

Xiong

et al. 2024

Ultrasonics Sonochemistry

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“…This solvent system could be adjusted to a broad polarity range, dissolving different targeted compounds. After combining with ultrasonic technology, a high extraction rate could be achieved, which was reported for the polysaccharides extraction from Indocalamus tessellatus leaves and Radix Bupleuri [13,14].…”

Section: Introductionmentioning

confidence: 92%

“…These results may be due to the fact that the ultrasound-assisted DES extraction could lead to hydrolytic disruption of polysaccharide chains and breakage of intermolecular hydrogen bonds, which affects the composition of monosaccharides [38]. Similarly, Zhang et al reported that three crude polysaccharides extracted from Indocalamus tessellatus leaves by hot water, ultrasonic and ultrasonic-assisted DES exhibited the same monosaccharide components but different molar ratios [13]. Thus, the different extraction methods had different impacts on the molar ratio of the monosaccharide composition of TPs.…”

Section: Monosaccharide Compositionsmentioning

confidence: 99%

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method

Xia

Liu

Sun

et al. 2023

Foods

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Deep eutectic solvent as a new green and safe solvent system has attracted more and more attention in recent years. In this study, three deep eutectic solvents (DES) were combined with ultrasound irradiation to extract tea polysaccharides (TPs) from Anji white tea, which was compared with conventional hot water extraction (HW). The physicochemical, structural, and biological properties of TPs extracted by ultrasound-assisted DES and hot water (HWP) were further investigated. Results showed that the DES system composed of choline chloride and 1,6-hexanediol (CH) with the molar ratio of 1:2 exhibited the optimal extraction yield (19.18%) and in vitro antioxidant activities for TPs (CHP). Furthermore, compared to the HWP, the CHP had a higher extraction yield and total carbohydrate content and a lower molecular weight. Monosaccharide composition analysis displayed that the molecular structure of CHP exhibited more arabinose but less glucose, mannose, galacturonic acid, and glucuronic acid than HWP. Little difference was observed in the preliminary structural characteristics between HWP and CHP from Fourier transform infrared analysis. Besides, CHP possessed better α-glucosidase inhibitory and hypoglycemic activity in L6 cells than HWP. Therefore, the ultrasound-assisted DES extraction method can be a promising strategy for extracting TPs with excellent bioactivities for future applications in functional foods.

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Optimization of ultrasonic-assisted deep eutectic solvent for the extraction of polysaccharides from Indocalamus tessellatus leaves and their biological studies

Cited by 20 publications

References 60 publications

Structural Characterization of Blueberry Fruit Polysaccharides and Assessment of its Hypolipidemic and Immunological Activity

Structural Characterization of Blueberry Fruit Polysaccharides and Assessment of its Hypolipidemic and Immunological Activity

Optimization of ultrasound-assisted extraction of Imperata cylindrica polysaccharides and evaluation of its anti-oxidant and amelioration of uric acid stimulated cell apoptosis

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method

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