Optimization of Ultrasonic-Assisted Extraction and Purification of Zeaxanthin and Lutein in Corn Gluten Meal

Wang, Litao; Lu, Weihang; Li, Jiali; Hu, Jinxia; Ding, Ruifang; Wang, Qibao

doi:10.3390/molecules24162994

Cited by 31 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CCD of RSM was performed to optimize the recovery conditions of lutein from T. suecica . RSM has the advantage of reducing the number of experiments and obtaining reliable data [ 30 ]. Three factors ( X 1 : temperature, X 2 : time and X 3 : S/L ratio) and five levels (temperature: 0, 15, 30, 45 and 60 °C; time: 1, 2, 3, 4 and 5 h; S/L ratio: 50, 75, 100, 125 and 150 g/L) were adopted for CCD.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

et al. 2021

View full text Add to dashboard Cite

Microalgae have been attracting attention as feedstock for biorefinery because they have various advantages, such as carbon fixation, high growth rate and high energy yield. The bioactive compounds and lutein contained in microalgae are known to be beneficial for human health, especially eye and brain health. In this study, in order to improve the recovery of bioactive extracts including lutein from Tetraselmis suecica with higher efficiency, an effective solvent was selected, and the extraction parameters such as temperature, time and solid loading were optimized by response surface methodology. The most effective solvent for lutein recovery was identified as 100% methanol, and the optimum condition was determined (42.4 °C, 4.0 h and 125 g/L biomass loading) by calculation of the multiple regression model. The maximum content of recovered lutein was found to be 2.79 mg/mL, and the ABTS radical scavenging activity (IC50) and ferric reducing antioxidant power (FRAP) value were about 3.36 mg/mL and 561.9 μmol/L, respectively. Finally, the maximum lutein recovery from T. suecica through statistical optimization was estimated to be 22.3 mg/g biomass, which was 3.1-fold improved compared to the control group.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Furthermore, the CCD of RSM was performed to investigate the correlation between the thermal-alkaline pretreatment and the three variables (X 1 : time, X 2 : KOH concentration and X 3 : solid loading) of five levels (time: 0, 10, 20, 30 and 40 min; KOH concentration: 0%, 1%, 2%, 3% and 4%; solid loading: 40, 60, 80, 100 and 120 g/L) [45]. A KOH concentration of 0% indicates pretreatment with DW and a reaction time of 0 min means a control group without thermal-alkaline pretreatment.…”

Section: Optimization Of Thermal-alkaline Pretreatment By Rsmmentioning

confidence: 99%

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

2021

View full text Add to dashboard Cite

Orange peel, which is a by-product of oranges, contains carbohydrates that can be converted into sugars and used in the fermentation process. In this study, the thermal alkaline pretreatment process was chosen because of its simplicity and lesser reaction time. In addition, the reaction factors were optimized using response surface methodology. The determined optimal conditions were as follows: 60.1 g/L orange peels loading, 3% KOH and 30 min. Under the optimal conditions, glucan content (GC) and enzymatic digestibility (ED) were found to be 32.8% and 87.8%, respectively. Enzymatic hydrolysis was performed with pretreated and non-pretreated orange peels using three types of enzyme complex (cellulase, cellobiase and xylanase). The minimum concentrations of enzyme complex required to obtain maximum ED were 30 FPU (filter paper unit), 15 CBU (cellobiase unit), and 30 XNU (xylanase unit) based on 1 g-biomass. Additionally, ED of the treated group was approximately 3.7-fold higher than that of the control group. In conclusion, the use of orange peel as a feedstock for biorefinery can be a strategic solution to reduce wastage of resources and produce sustainable bioproducts.

show abstract

“…Separations 2021, 8, x FOR PEER REVIEW 3 of 18 the extraction conditions for zeaxanthin, lutein epoxide, and violaxanthin, which were based on our preliminary experiments. Furthermore, with regard to optimizing extraction factors with RSM, total carotenoids extracted from Aresch [35] by subcritical fluid extraction was 0.239 g/kg, zeaxanthin and lutein from corn gluten by UAE were separated and purified using silica gel column chromatography with the purity of zeaxanthin increasing from 0.28% to 31.5% (about 110 times) and lutein from 0.25% to 16.3% (about 65 times) [36]. Using the high hydrostatic-pressure-assisted extraction method, 2.01 ± 0.09 mg/100 g of lycopene was obtained from the tomato [37], so, different methods with various species contributed several yields.…”

Section: Introductionmentioning

confidence: 99%

Optimum Parameters for Extracting Three Kinds of Carotenoids from Pepper Leaves by Response Surface Methodology

Ding

et al. 2021

Separations

View full text Add to dashboard Cite

To determine the optimum parameters for extracting three carotenoids including zeaxanthin, lutein epoxide, and violaxanthin from pepper leaves by response surface methodology (RSM), a solvent of acetone and ethyl acetate (1:2) was used to extract carotenoids with four independent factors: ultrasound time (20–60 min); ratio of sample to solvent (1:12–1:4); saponification time (10–50 min); and concentration of saponification solution (KOH–methanol) (10–30%). A second-order polynomial model produced a satisfactory fitting of the experimental data with regard to zeaxanthin (R2 = 75.95%, p < 0.0197), lutein epoxide (R2 = 90.24%, p < 0.0001), and violaxanthin (R2 = 73.84%, p < 0.0809) content. The optimum joint extraction conditions of zeaxanthin, lutein epoxide, and violaxanthin were 40 min, 1:8, 32 min, and 20%, respectively. The optimal predicted contents for zeaxanthin (0.823022 µg/g DW), lutein epoxide (4.03684 µg/g dry; DW—dry weight), and violaxanthin (16.1972 µg/g DW) in extraction had little difference with the actual experimental values obtained under the optimum extraction conditions for each response: zeaxanthin (0.8118 µg/g DW), lutein epoxide (3.9497 µg/g DW), and violaxanthin (16.1590 µg/g DW), which provides a theoretical basis and method for cultivating new varieties at low temperatures and weak light resistance.

show abstract

Optimization of Ultrasonic-Assisted Extraction and Purification of Zeaxanthin and Lutein in Corn Gluten Meal

Cited by 31 publications

References 28 publications

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

Optimum Parameters for Extracting Three Kinds of Carotenoids from Pepper Leaves by Response Surface Methodology

Contact Info

Product

Resources

About