Optimization of ultrasonic-assisted extraction of flavonoids from Lactuca indica L. cv. Mengzao and their antioxidant properties

Abstract

In this study, the ultrasound-assisted extraction (UAE) conditions of flavonoids from Lactuca indica L.cv. Mengzao (LIM) leaves was optimized, and the flavonoids content and their antioxidant potential in different parts were analyzed. The optimal extraction parameters to obtain the highest total flavonoids content (TFC) were a a ratio of liquid to solid of 24.76 mL/g, ultrasonic power of 411.43 W, ethanol concentration of 58.86% and an extraction time of 30 min, the average TFC of LIM leaves could reach 48.01 mg/g. For the yield of flavonoids, the UAE method had the best extraction capacity compared with solvent extraction and microwave-assisted extraction (MAE). In general, the TFC in different parts of LIM followed the order flower > leaf > stem > root, the flowering period is the most suitable harvesting period. From ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS) quantification, the flower samples showed significantly higher six flavonoids and had the highest radical scavenging capacities compared to other samples. A high positive correlation was observed between the antioxidant activity and TFC, luteolin-7-O-glucoside and rutin were significantly (p < 0.05) correlated with all antioxidant evaluations. This study provides valuable information for the development and utilization of flavonoids in Lactuca indica as ingredients in food, feed and nutritional health products.

Ultrasonic extraction of Inula viscosa: Enhancing antioxidant bioactivity and its application in sunflower oil as an antioxidant

“…This is because alcohol-soluble pigments and lipophilic components may dissolve more at higher ethanol concentrations. This makes it less likely for flavonoids to bind with ethanol–water molecules, which slows the extraction process [28] .…”

Extraction of flavonoids from durian (Durio zibethinus) fruit rinds and evaluation of their antioxidant, antidiabetic and anticancer properties

“…These results were similar to those for flavonoid extraction from Lactuca indica L. cv. Mengzao (Hao et al ., 2023). This behaviour could be due to the solvent polarity, which affects the efficiency of the phytochemical extraction (Do et al ., 2014; Alara et al ., 2020; Sucipto et al ., 2021).…”

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

“…The extraction of phenolic compounds from natural products has been conducted with several methods, such as maceration [68], percolation [69], Soxhlet extraction [68], reflux extraction [69], decoction [69], ultrasound-assisted extraction (UAE) [70], pulsed electric filed extraction (PEF) [71], enzyme-assisted extraction (EAE) [72], microwave-assisted extraction (MAE) [73], homogenate-assisted extraction (HAE) [74], and subcritical water extraction (SWE) [75]. An extract from plant material is a complex mixture containing different types of natural compounds with different polarities.…”