Optimization of ultrasound-assisted extraction of procyanidins from perilla seed hull and their antioxidant activities in vitro

Abstract

Ultrasound-assisted extraction (UAE) was used to extract procyanidins (PC) from the perilla seed hull. Four independent variables, namely, liquid-to-solid ratio, ethanol concentration, UAE time, and UAE temperature were investigated. Response surface methodology was performed on the basis of results of single-factor tests. The optimal conditions for PC extraction were as follows: a liquid-to-solid ratio of 15:1, an ethanol concentration of 62%, a UAE time of 29 min, and a UAE temperature of 53 °C. Under these conditions, the experimental yield of PC was 2.185 mg/g (n = 3), which is correlated with the predicted value of 2.2 mg/g. The extracted and purified PC showed excellent antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radicals in vitro.

Optimization of Ultrasonic-Assisted Bioactive Compound Extraction from Green Soybean (Glycine max L.) and the Effect of Drying Methods and Storage Conditions on Procyanidin Extract

“…Antioxidants have a well-established role in preventive medicine. A surge in interest in the discovery of novel, safe, non-toxic, and valuably effective organic antioxidants has occurred as a result of their numerous benefits, such as anti-aging and anti-inflammatory properties [ 3 ].…”

“…A study has determined that higher procyanidin consumption is related to a lower cancer risk. Based on their health advantages, procyanidins are potentially beneficial as dietary antioxidants and nutraceuticals [ 3 ]. Typically, procyanidins are derived from several natural sources, including apples, cinnamon, grape peels, grape seeds, legumes, and maritime pine bark [ 5 ].…”

Berberis crataegina DC. as a novel natural food colorant source: ultrasound-assisted extraction optimization using response surface methodology and thermal stability studies

“…The UAE method shortens the extraction time and energy requirements as compared to conventional extraction methods. Also, the UAE method provides the recovery of bioactive compounds through the action of acoustic cavitation generated by an ultrasound wave in the solvent, and the enhancement of mass transport by disrupting the plant cell walls (Dranca & Oroian, 2016;Li et al, 2019;Riciputi et al, 2018;Rodrigues et al, 2015). On the other hand, the UAE efficiency is affected by several parameters such as ultrasound amplitude, extraction time, temperature, solvent-sample ratio, and pH (Chemat et al, 2017).…”

“…with a response surface methodology (RSM), which consists of mathematical techniques to maximize the analytical response (Ding et al, 2016;Li et al, 2019). However, to the best of our knowledge, there is no literature on the UAE of TPC and TAC from Berberis crataegina DC.…”

Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells

“…Perilla seed meal (PSM), a by-product of PSO extraction process, is discarded as a waste product or used as a protein source in animal feed as a low-cost material [14][15][16]. The PSM should be analyzed and reused because it is rich in numerous bioactive components such as phenolic compounds and others [17][18][19][20]. Because their molecular mechanisms remain unknown, it is worth studying these active pharmaceutical ingredients in PSO and PSM.…”