Optimization of ultrasound‐assisted extraction parameters for improving content of acteoside, luteolin‐7‐ O ‐glucoside, and total polyphenols in extracts of Plantago lanceolata aerial parts

Abstract

This study was designed in order to determine optimal parameters for ultrasoundassisted extraction (UAE) of polyphenols from plantain herb using response surface methodology (RSM) as well as UV-visible spectrophotometric and reverse-phase high-performance liquid chromatographic (RP-HPLC) method. Solvent extraction was performed based on four factors-five levels central composite design (CCD). The influence of extraction time (X 1 : 5-65 min), ethanol concentration (X 2 : 10%-90%), solid to solvent ratio (X 3 : 1:10-1:50), and extraction temperature (X 4 : 20-80°C) on total polyphenols content (TPC) as well on content of dominant individual polyphenolic compounds (acteoside and luteolin-7-O-glucoside) was analyzed. The optimal extraction parameters were as follows: extraction time of 64 min, ethanol concentration of 45%, solid to solvent ratio of 1:49, and extraction temperature of 40°C. Under these optimal conditions, the experimental values for total phenolics and acteoside and luteolin-7-O-glucoside content in plantain herb extract were 42.15, 24.15, and 0.87 mg/g DW, respectively. Experimental validation of optimized parameters was performed and close agreement with predicted values suggested the effectiveness of the applied model and the achievement of RSM in optimizing the extraction parameters.

Efficient Recovery Strategy of Luteolin from Agricultural Waste Peanut Shells and Activity Evaluation of Its Functional Biomolecules

“…Extraction techniques such as enzyme-assisted extraction, Recent studies on luteolin recovery from biomass are summarized in Table 8. The luteolin recovery was performed from biomass such as carrot [20], Cretan brake fern [34], deulkkae [19], leaves of olive trees [35,36], pigeon pea [37], PSs [18] and ribwort plantain [38] using various extraction methods. Among them, PSs have a higher luteolin content than any other biomass.…”