Optimization of ultrasound‐assisted solvent extraction of hemp (<i>Cannabis sativa</i> L.) seed oil using RSM: Evaluation of oxidative stability and physicochemical properties of oil

Kenari, Reza Esmaeilzadeh; Dehghan, Bahareh

doi:10.1002/fsn3.1796

Cited by 41 publications

(17 citation statements)

References 34 publications

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“…These results indicated good fit between the values predicted experimentally and by the model that was applied. Regarding the values of CV obtained for all the investigated responses, presented in Table 3, it was noticed that a good fit of the model applied was obtained for all responses due to relatively low CV (1.68, 3.03, 4.80, 6.34, 10.04, 4.46 and 4.61 for Y, TP, TF, TMAC, DPPH, ABTS and FRAP, respectively), since low CV values indicate the good accordance and reliability of experiments and practical works [40]. According to the results of ANOVA presented in Table 3, a good fit between experimental and predicted values was observed.…”

Section: Defining the Influence Of Process Parameters Extraction Process Modelingmentioning

confidence: 88%

Maximizing Contents of Phytochemicals Obtained from Dried Sour Cherries by Ultrasound-Assisted Extraction

et al. 2021

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Sour cherries were first dried by vacuum drying and then used as material for obtaining extracts rich in bioactive compounds by ultrasound-assisted extraction (UAE). The first step was to apply a factorial design for the preliminary experiments to determine the most influential UAE factors, and thus the three studied parameters were chosen as the most suitable for the design of the main experiment (temperature, liquid–solid ratio and ethanol concentration). In this part, the contents of total phenols and the total content of monomeric anthocyanins were taken for responses. For the further optimization of UAE, experimental design (face-centered) was applied, and the yield, total phenolics, flavonoid content and content of monomeric anthocyanins and antioxidant activity (DPPH, ABTS and FRAP assays) were analyzed. Temperature (40–80 °C), ethanol concentration (40–80%, w/w) and liquid–solid ratio (10–30 mL/g) were investigated as independent variables. The obtained experimental results were fitted to a second-order polynomial model and analysis of variance was used to determine the fit of the model and the optimal conditions for investigated responses. High quality extracts with high concentrations of polyphenols and anthocyanins were also obtained, which could be used as food additives.

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Section: Defining the Influence Of Process Parameters Extraction Process Modelingmentioning

confidence: 88%

Maximizing Contents of Phytochemicals Obtained from Dried Sour Cherries by Ultrasound-Assisted Extraction

et al. 2021

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“…The biological activities of hemp seed oil, and especially the effects on the oxidative state under non-stress and oxidative-stress conditions, have been poorly studied in animal models, despite the unique chemical composition. The accumulated results reveal the antioxidant potential of the oil [ 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Fotschki et al [ 29 ] reported that hemp seed oil induced oxidative stress in livers of obese rats, while in healthy rats it positively affected the antioxidant status.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Hemp (Cannabis sativa L.) Seed Oil in Drosophila melanogaster Larvae under Non-Stress and H2O2-Induced Oxidative Stress Conditions

et al. 2021

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The oil extracted from hemp seeds has significant nutritional and biological properties due to the unique composition of polyunsaturated fatty acids and various antioxidant compounds. The potential of this oil for the prevention of oxidative stress and for the treatment of oxidative-stress-induced ailments is of increasing interest. Most studies of hemp seed oil were conducted in-vitro, meaning we lack information about effects and activity in vivo. In the present study, we evaluated the hypothesis that hemp seed oil at different concentrations improves the oxidative state of D. melanogaster, under non-stress as well as hydrogen-peroxide-induced stress. We analyzed the effects of hemp seed oil on oxidative stress markers and on the life cycle of D. melanogaster under non-stress and hydrogen-peroxide-induced stress conditions. D. melanogaster larvae were exposed to hemp seed oil concentrations ranging from 12.5 to 125 μL/mL. The results revealed that under non-stress conditions, oil concentrations up to 62.5 µL/mL did not induce negative effects on the life cycle of D. melanogaster and maintained the redox status of the larval cells at similar levels to the control level. Under oxidative stress conditions, biochemical parameters were significantly affected and only two oil concentrations, 18.7 and 31.2 µL/mL, provided protection against hydrogen peroxide stress effects. A higher oil concentration (125 μL/mL) exerted negative effects on the oxidative status and increased larval mortality. The tested oil was characterized chemically by NMR, transesterification, and silylation, followed by GC-MS analyses, and was shown to contain polyunsaturated fatty acid triglycerides and low levels of tocopherols. The high levels of linoleic and linolenic acids in the oil are suggested to be responsible for the observed in vivo antioxidant effects. Taken together, the results show that hemp seed oil is effective for reducing oxidative stress at the cellular level, thus supporting the hypothesis. The obtained results point to the potential of hemp seed oil for the prevention and treatment of conditions caused by the action of reactive oxygen species.

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“…In contrast, wet and dry rendering permits the degradation of the phenolic compounds by oxidation or hydrolysis due to the application of high temperature (≥103 • C) in addition to time-consuming (80 min) extraction [7,8]. In previous reports on bio-oil extractions, a higher temperature and prolonged extraction time contributed to the deterioration of the phenolic compounds and decreased the antioxidant activity of the resulting oils [9][10][11]. Traditionally, water is employed as the extraction solvent by the local RFO producer.…”

Section: Introductionmentioning

confidence: 99%

“…Among these techniques, UAE seems to be the most suitable for extracting oil containing high phenolic compounds from red fruit, as a moderate temperature is applied. Additionally, UAE has been proven successful in extracting phenolic-rich oil from radish seed [12], Moringa peregrina seed [9], Cannabis sativa seed [10], and olive [13].…”

Section: Introductionmentioning

confidence: 99%

Dual Response Optimization of Ultrasound-Assisted Oil Extraction from Red Fruit (Pandanus conoideus): Recovery and Total Phenolic Compounds

2022

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Red fruit oil (RFO) is a high-value oil that contains functional compounds, mainly phenolic compounds, providing antioxidant activity. Therefore, an optimal extraction method is essential to recover the RFO and phenolic compounds simultaneously. This research aimed to optimize the ultrasound-assisted extraction (UAE) for oil from red fruit using the Box-Behnken design combined with response surface methodology. The studied UAE factors, including sample-to-solvent ratio (1:3, 1:2, and 1:1 g mL−1), extraction temperature (60, 75, and 90 °C), and pulse duty-cycle (0.20, 0.50, and 0.80 s−1). Analysis of variance revealed that the three studied factors significantly influenced the recovered RFO, while the level of total phenolic compounds in the extracts was defined merely by extraction temperature (p < 0.05). These significant factors were then included in the optimization models (R2 > 0.99, lack-of-fit p > 0.05). The proposed UAE setting by the multiresponse optimization was an extraction temperature of 67 °C, a pulse duty-cycle of 0.50 s−1, and a sample-to-solvent ratio of 1:2.5 g mL−1. Subsequently, the extraction kinetic was evaluated, confirming full recovery at 60 min of extraction time. The developed method was then applied to extract six red fruit clones. Mbarugum clones provided high RFO recovery (9.60%), with an uppermost total phenolic compound of (42.63 mg GAE g−1) among the six red fruit clones. Additionally, the resulting RFO showed eminent antioxidant activities, indicated by excellent values of IC50 DPPH (37.69 mg L−1), IC50 FIC (30.43 mg L−1), FRAP reducing power (63.55 mg AAEA g−1), and IC50 ABTS (93.88 mg L−1). In contrast with a wet rendering method, UAE enhanced the RFO recovery by 53.02%, resulting in a higher level of total phenolic compounds. Henceforth, the proposed UAE method is a promising technique to substitute conventional oil production in the food and pharmaceutical industries.

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Optimization of ultrasound‐assisted solvent extraction of hemp (Cannabis sativa L.) seed oil using RSM: Evaluation of oxidative stability and physicochemical properties of oil

Cited by 41 publications

References 34 publications

Maximizing Contents of Phytochemicals Obtained from Dried Sour Cherries by Ultrasound-Assisted Extraction

Maximizing Contents of Phytochemicals Obtained from Dried Sour Cherries by Ultrasound-Assisted Extraction

Antioxidant Activity of Hemp (Cannabis sativa L.) Seed Oil in Drosophila melanogaster Larvae under Non-Stress and H2O2-Induced Oxidative Stress Conditions

Dual Response Optimization of Ultrasound-Assisted Oil Extraction from Red Fruit (Pandanus conoideus): Recovery and Total Phenolic Compounds

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