Optimization of Various Growth Media to Freshwater Microalgae for Biomass Production

Ilavarasi, A.; MubarakAli, D.; Ramasamy, P.; Baldev, E.; Thajuddin, Nooruddin

doi:10.3923/biotech.2011.540.545

Cited by 87 publications

(23 citation statements)

References 12 publications

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“…Thus, the use of at least two types of medium, enrichment medium for the growth of general fast-growing fungi and minimal medium for the isolation of slow-growing fungi, might ensure the isolation of fungal strains with different growth rates. The BBM was designed originally for the cultivation of green algae [ 71 ]. This carbon-deficient medium made it possible to isolate slow-growing fungi, including Cladophialophora aff.…”

Section: Discussionmentioning

confidence: 99%

Effect of Isolation Conditions on Diversity of Endolichenic Fungal Communities from a Foliose Lichen, Parmotrema tinctorum

Yang

Kim

et al. 2021

JoF

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Endolichenic fungi (ELF) are emerging novel bioresources because their diverse secondary metabolites have a wide range of biological activities. Metagenomic analysis of lichen thalli demonstrated that the conventional isolation method of ELF covers a very limited range of ELF, and the development of an advanced isolation method is needed. The influence of four variables were investigated in this study to determine the suitable conditions for the isolation of more diverse ELF from a radially growing foliose lichen, Parmotrema tinctorum. Four variables were tested: age of the thallus, severity of surface-sterilization of the thallus, size of a thallus fragment for the inoculation, and nutrient requirement. In total, 104 species (1885 strains) of ELF were isolated from the five individual thalli of P. tinctorum collected at five different places. Most of the ELF isolates belong to Sordariomycetes. Because each part of lichen thallus (of different age) has unique ELF species, the whole thallus of the foliose lichen is needed to isolate diverse ELF. Moderate sterilization is appropriate for the isolation of diverse ELF. Inoculation of small fragment (1 mm2) of lichen thallus resulted in the isolation of highest diversity of ELF species compared to larger fragments (100 and 25 mm2). Moreover, ELF species isolated from the small thallus fragments covered all ELF taxa detected from the medium and the large fragments in this study. The use of two media—Bold’s basal medium (nutrient poor) and potato dextrose agar (nutrient rich)—supported the isolation of diverse ELF. Among the tested variables, size of thallus fragment more significantly influenced the isolation of diverse ELF than other three factors. Species composition and richness of ELF communities from different lichen thalli differed from each other in this study.

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Section: Discussionmentioning

confidence: 99%

Effect of Isolation Conditions on Diversity of Endolichenic Fungal Communities from a Foliose Lichen, Parmotrema tinctorum

Yang

Kim

et al. 2021

JoF

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“…The Chlorella vulgaris ( C. vulgaris ) strain (CCAP 211/63) was obtained from Culture Collection of Algae and Protozoa, UK. The C. vulgaris strain was cultured in Bold's Basal Medium in 10 l Nalgene carboys at 19°C under artificial light with a 16:8 light dark photoperiod [24]. After 30 days of culture, microalgae were first concentrated by sedimentation for 48 h at 4°C, and then the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

Renewable energy: evaluation of low energy demand pre‐treatments to optimise methane production from microalgae

Zhang

Caldwell

Sallis

2019

IET Renewable Power Generation

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Anaerobic digestion (AD) of microalgae is a sustainable process to produce methane-rich biogas for bioenergy production. However, the rigid cell walls of microalgae protect them against the attack from hydrolytic bacteria, and consequently prevent efficient biodegradability and lower methane production. In this study, the effects of low energy demand enzymatic and low-temperature thermo-alkaline pre-treatments on microalgae AD were investigated in batch biochemical methane potential tests. The results found that methane yields were significantly enhanced by both pre-treatments. Microalgae Chlorella vulgaris (C. vulgaris) after enzymatic pre-treatment enhanced methane yields the most, by 22-162%, whilst C. vulgaris pre-treated by thermo-alkaline pre-treatment improved methane yields by 4-26%. In enzymatic pre-treatment, C. vulgaris pretreated with mixed enzymes showed higher methane yields compared to single enzymes. In low-temperature thermo-alkaline pre-treatment, the level of enhancement in methane yields depended on the alkaline dosage and pre-treatment temperature, but the high alkaline dosages were associated with limitations such as a prolonged lag phase in the digestion process. From an energy viewpoint, both pre-treatments showed positive energy balances for the majority of experimental conditions, and therefore both pre-treatments are considered to be energetically efficient methods to pre-treat microalgae for methane production via AD.

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“…A total of 17 mL of microlalgae were inoculated into BBM medium as much as 153 mL and given aeration at room temperature. Microalgae growth was determined by measuring the optical density of the culture every day using a UV-Vis spectrophotometer at a wavelength of 410 nm 10 .…”

Section: Bbm Mediummentioning

confidence: 99%

Isolation and Selection of Growth Medium for Microalgae of Lake Biru Sawahlunto West Sumatra and Antibacterial Activity Test

Chaidir¹,

Rahmayuni²,

Djamaan³

2019

J Pure Appl Microbiol

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This research was done to overcome the bacterial resistance towards antibiotics by produced new antibiotics. Some microorganisms can be used as a source of antibiotics, including microalgae. This study was conducted by using microalgae Scenedesmus dimorphus to produced a new antibiotic. The study began by looking for a suitable growth medium to obtain maximum biomass to produces antibiotics. The results showed that the suitable growth medium was Bolt Basalt Medium (BBM) with dry biomass of 0.602 g/L, whereas from grow more G32% medium was obtained 0.302 g/L and commercial fertilizer P5 was obtained 0.479 g/L. The antibacterial activity of Scenedesmus dimorphus extract was tested using the disc diffusion method (Kirby & Bauer test). The extraction process using methanol, ethyl acetate and hexane solvents was carried out by the maceration method with the help of the sonication process. Antibacterial activity testing showed the inhibition zone on methanol extract using Bacillus subtilis ATCC 6633, Salmonella thypimurium ATCC 14028, Eschericia coli AtCC 25922, Methicilin Resistent-Staphylococus aureus ATCC 25175 respectively 13 mm, 10 mm, 10 mm, 20 mm while on ethyl acetate extracts the inhibition zone size were 9 mm, 8 mm, 11 mm, 12 mm and n-hexane extracts were 8 mm, 7 mm, 8 mm and 8 mm.

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Optimization of Various Growth Media to Freshwater Microalgae for Biomass Production

Cited by 87 publications

References 12 publications

Effect of Isolation Conditions on Diversity of Endolichenic Fungal Communities from a Foliose Lichen, Parmotrema tinctorum

Effect of Isolation Conditions on Diversity of Endolichenic Fungal Communities from a Foliose Lichen, Parmotrema tinctorum

Renewable energy: evaluation of low energy demand pre‐treatments to optimise methane production from microalgae

Isolation and Selection of Growth Medium for Microalgae of Lake Biru Sawahlunto West Sumatra and Antibacterial Activity Test

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