“…RSM is suitable for identifying the effect of individual variables and for efficiently seeking the conditions for an optimum response in a multivariable system (Montgomery, 2001). This method has been successfully applied to optimize fermentation processes (Maddox and Reichert, 1997;Chen, 1981;Grothe et al, 1999;Ratnam et al, 2003;Casas López et al, 2004), vegetable oil bioconversion (Cheynier et al, 1983), biomass production (Moresi et al, 1980), and numerous other processes (Panda et al, 1999;Ellaiah et al, 2002;Adinarayaan and Suren, 2005). Effects of temperature, pH, and enzyme dosage on sago starch hydrolysis using bacterial a-amylase, b-glucanase, and pullulanase have been previously studied using statistical design of experiment (Gorinstein et al, 1994).…”