Optimization of Xylanase Production by Trichoderma orientalis Using Corn Cobs and Wheat Bran via Statistical Strategy

Long, Chuannan; Liu, Jian; Gan, Lihui; Zeng, Bin; Long, Minnan

doi:10.1007/s12649-017-0149-x

Cited by 22 publications

(18 citation statements)

References 23 publications

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“…The findings of the Plackett–Burman study were in agreement with other studies based on xylanase production. For example, MgSO 4 has been reported by several researchers to have a positive influence on xylanase production by bacterial and fungal species alike [21,22]. Furthermore, several studies have reported the importance of KH 2 PO 4 in xylanase production media.…”

Section: Resultsmentioning

confidence: 99%

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Ravindran¹,

Williams²,

Jaiswal³

2019

Foods

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In this study, spent coffee waste (SCW) was used as the sole carbon source for xylanase production in solid state fermentation mode using Aspergillus niger. A Box–Behnken design was constructed using three parameters viz. temperature, initial moisture content, and log number of spores to determine the optimal fermentation condition. The best fermentation conditions for xylanase production were found to be incubation at 30 °C with an initial moisture content of 70% and using an inoculum of 6.5 × 106 spores/g of dry SCW. Furthermore, the design of experiments revealed that maintaining a medium composition of 0.2 g of yeast extract, 0.04 g of K2HPO4, and 0.03 g of MgSO4 increased xylanase production. Under optimised solid-state fermentation conditions an enzyme activity of 6495.6 IU/g of dry SCW was recorded, which was approximately 1.39-fold higher than that of control (4649 IU/g of dry SCW). The efficacy of the purified xylanase as a juice enrichment agent for strawberry, blueberry, and raspberry pulp was tested.

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Section: Resultsmentioning

confidence: 99%

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Ravindran¹,

Williams²,

Jaiswal³

2019

Foods

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“…Commonly, microbes are acknowledged to yield high levels of enzymes at their optimal growth temperature. Long et al [ 16 ] informed that the optimal temperature intended for xylanase enzyme production from Trichoderma orientalis was 37.3 °C while the ideal temperature for xylanase enzyme invention from Trichoderma sp. was 30 °C [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

“…Amongst microbial sources, the genus Trichoderma is, widely known to supply an excessive amount of xylanase enzyme, can be added to the culture medium. This property makes fungi economically powerful manufacturers of xylanases [ 15 , 16 , 17 ]. Trichoderma strains are non-plant pathogenic filamentous fungi that can generate an excessive yield of extracellular xylan-degrading enzymes [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Production, Cloning, and Expression of a Xylanase Gene from Endophytic Fungal Strain Trichoderma harzianum kj831197.1: Unveiling the In Vitro Anti-Fungal Activity against Phytopathogenic Fungi

Ellatif

Razik

AL-surhanee

et al. 2022

JoF

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Trichoderma sp. is extensively applied as a beneficial fungus for the management of plant diseases, plant growth promotion, induced resistance, and plays an important role in global sustainable agriculture. This study aimed to enhance the production of microbial xylanase in high titer from the endophytic fungus Trichoderma harzianum kj831197.1, and the cloning of xylanase genes in E. coli DH5α using a pUC19 vector. A combination of glucose, 0.1 mM, Tween 80 with lactose, and 2 mM galactose combined with malt extract boostedthe enzyme production. Xylanase production was maximized at a pH of 5.0, temp. of 30 °C, and agitation of 150 rpm in the presence of malt extract and bagasse as the best nitrogen source and waste, respectively, using submerged fermentation. The molecular weight of highly purified xylanase was 32 KDa, identified using SDS-PAGE. The xylanase gene of T. harzianum kj831197.1 was screened in fungal DNA using definite primers specified in the gene bank database. The identified region was excised using restriction enzymes HindIII and EcoRI and cloned into a pUC19 plasmid vector. Optimization of fermentation conditions improved xylanase production about 23.9-fold.The antifungal efficacy of xylanase toward different phytopathogenic fungi was determined. The highest inhibition was against Corynespora cassiicola, Alternaria sp., Fusarium oxysporum, and Botrytis fabae. This study offered an economical, simple, and efficient method using Trichoderma harzianum kj831197.1 for the production of the xylanase enzyme via the submerged fermentation method.

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“…The OFAT approach is a conventional single-dimensional investigation that involves changing one independent variable at a time while the others remain at their optimal level 19 , 20 . This is the main strategy used for selecting optimal conditions, which continues to be widely used in preliminary optimization studies 21 . The main disadvantages of OFAT are the partial explanation regarding the effect of the factors on the response and the absence of the interaction effects between the variables 22 .…”

Section: Introductionmentioning

confidence: 99%

Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy

Dhaver

Pletschke

Sitholé

et al. 2022

Sci Rep

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Xylanases are hydrolytic enzymes with a wide range of applications in several industries such as biofuels, paper and pulp, food, and feed. The objective of this study was to optimize the culture conditions and medium components for maximal xylanase production from a newly isolated Trichoderma harzianum strain using the Plackett–Burman Design (PBD) and Box Behnken Design (BBD) experimental strategies. Xylanase production was enhanced 4.16-fold to 153.80 U/ml by BBD compared to a preliminary one-factor-at-a-time (OFAT) activity of 37.01 U/ml and 2.24-fold compared to the PBD (68.70 U/ml). The optimal conditions for xylanase production were: 6 days of fermentation, incubation temperature of 70 °C, pH 5.0, agitation of 160 rpm, and 1.2% wheat bran and ammonium sulphate. The experimental design effectively provided conditions for the production of an acidic-thermostable enzyme with exciting potential for application in animal feed improvement. The acidic-thermostable xylanase was purified from the submerged culture and SDS-PAGE analysis revealed a molecular weight of 72 kDa. This protein had maximum xylanolytic activity at pH 6.0 and 65 °C and was stable for 4 h retaining > 70% activity and exhibited substrate specificity for beechwood xylan with a Km of 5.56 mg/ml and Vmax of 1052.63 µmol/min/mg. Enzyme activity was enhanced by Fe2+, Mg2+, and Zn2+. There was an absence of strong inhibitors of xylanase activity. Overall, these characteristics indicate the potential for at least two industrial applications.

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Optimization of Xylanase Production by Trichoderma orientalis Using Corn Cobs and Wheat Bran via Statistical Strategy

Cited by 22 publications

References 23 publications

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Enhanced Production, Cloning, and Expression of a Xylanase Gene from Endophytic Fungal Strain Trichoderma harzianum kj831197.1: Unveiling the In Vitro Anti-Fungal Activity against Phytopathogenic Fungi

Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy

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