Optimization of Xylanase Production byThermomyces lanuginosusin Solid State Fermentation

Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase

“…It is clearly evident from Figure 2(b) that urea adversely affected β-mannanase production. These results are well supported by the earlier reports on hemicellulase production where organic nitrogen sources, particularly, yeast extract has been found to enhance enzyme production (Gaffney et al 2009) while urea supplementation repressed β-mannanase production (Betini et al 2009). …”

Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches

“…However in this study, xylanase production was done in fermentation medium composed of lignocellulosic substrate (OPEFB) mixed with respective pure simple sugars as carbon sources. Conversely, other than this study, some other reports on xylanase production using lignocellulosic media (Gaffney et al 2009;Lakshmi et al 2011), have observed that the repression of xylanase by the addition of easily-metabolised sugars-including xylose-only occurred at sugar concentrations higher than 2% and 3% (w/w) of the lignocellulosic medium. But their reports did not clarify why xylanase synthesis was repressed only at higher reducing sugar concentrations.…”

“…However, the inductive ability of a mixture of lignocellulosic substrate and easilymetabolised sugars as carbon source for lignocellulosic enzyme production has been poorly investigated, and the mechanism of observed responses is yet unclear. Incidentally, some few earlier researchers (Lakshmi et al 2009;Gaffney et al 2009) have reported the effect of this mixture on xylanase production; all were however plagued with conflicting results. In fact, none of the reports could show the mechanism or at least provide sufficient explanation for their respective observations.…”

Endoglucanase production with the newly isolated Myceliophtora sp. i-1d3b in a packed bed solid state fermentor

“…Researches in SSF using thermophilic fungi to produce enzymes are increasing due to their intrinsic stability (17), and some thermophilic fungi have been recently reported as good producers of cellulolytic complexes enzymes in experiments carried out in glass flasks or in plastic bags (4,6,9,10,15,16,26). However, it is not recommended that the optimal experimental conditions found in glass flasks tests be immediately transposed to the bioreactors tests, since the dynamic conditions observed in the fermentors are quite different (1).…”