Optimization of xylanase production by Penicillium citrinum xym2 and application in saccharification of agro-residues

Saha, Shyama Prasad; Ghosh, Shilpi

doi:10.1016/j.bcab.2014.03.003

Cited by 43 publications

(29 citation statements)

References 26 publications

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“…For most of the fungus including Penicillium sp. the optimum temperature is 30 • C and outside that mesophilic range, there could be decrease in the product yield which in contrast to present report where the optimum temperature was found to be 40 • C for enzyme production (Saha and Ghosh, 2014). This might be the reason for low yield of xylanase with unoptimized conditions where the temperature was maintained at mesophilic range (30 • C).…”

Section: Discussioncontrasting

confidence: 84%

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

2020

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Corn cob is an abundant organic source with significant potential in sustainable energy development. For the effective conversion of the feedstocks to valued commodities, effective biocatalysts are highly desired. The present study aims at optimizing the critical parameters required for xylanase production by Penicillium purpurogenum isolated from rotten wood sample using the Taguchi orthogonal array layout of L25 (5 ∧ 6). The optimized conditions like temperature 40 • C, pH 3, size of inoculum 1.2 × 10 8 spores/ml, moisture 70%, peptone 0.8%, and 5 days of incubation resulted in 1,097 ± 6.76 U/gram dry substrate (gds) xylanase which was 65.72% more when compared to un-optimized production of xylanase. The xylanase thus produced, effectively carried out pretreated corn cob saccharification and the reaction was further improved with ultrasound assistance which has increased the saccharification yield to 12.02% along with significant reduction in reaction time. The saccharification efficiency of pretreated corn cob was found to be 80.29% more compared to the raw corn cob, reflecting its recalcitrance to digestion. Indeed, xylan being the second most abundant polymer in lignocellulosic biomass, considerable attention is being paid for its effective conversion to valued products.

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Section: Discussioncontrasting

confidence: 84%

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

2020

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“…Similar results were also found in previous studies (Saha and Ghosh 2014;Kocabas et al 2015). A reasonable explanation is that the xylanases from different organisms have different substrate specificities (Saha and Ghosh 2014). In this work, a relatively high yield (75%) of alkaline pretreated wheat bran demonstrated its potential in saccharification of second generation feed stocks into sugars.…”

Section: Saccharification Of Biomass Wastessupporting

confidence: 90%

“…The results showed that the crude enzyme exhibited obviously different saccharification rates for several biomass wastes. Similar results were also found in previous studies (Saha and Ghosh 2014;Kocabas et al 2015). A reasonable explanation is that the xylanases from different organisms have different substrate specificities (Saha and Ghosh 2014).…”

Section: Saccharification Of Biomass Wastessupporting

confidence: 90%

Purification and Characterization of Xylanase from Spent Mushroom Compost and its Application in Saccharification of Biomass Wastes

Lin

Sun

et al. 2017

BioResources

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The activities of xylanase extracted from spent mushroom composts (SMCs) of Coprinus comatus, Auricularia auricular, Pleurotus ostreatus, Pleurotus citrinopileatus, Agrocybe cylindracea, Hericium erinaceus, Hypsizygus marmoreus, and Tremella fuciformis were investigated. The crude extract from T. fuciformis SMC showed high xylanase activity with a value of 255.2 U/mg. Furthermore, this xylanase was purified using a combination of ammonium sulfate precipitation, diethylaminoethylcellulose (DEAE-cellulose), and gel filtration column chromatography. The enzyme was purified 20.7-fold with a yield of 43.1% and activity of 5293.8 U/mg. The purified xylanase showed maximum activity at 50 °C and pH 6, retained 80% activity after 1 h incubation at 50 °C, and sustained stability over a wide range of pH values (2 to 10). Under the optimal conditions, the enzyme exhibited a Km value of 2.5 mg/mL towards birchwood xylan. The activity of xylanase was enhanced in the presence of Mg 2+ , Ca 2+ , Ba 2+ , NH4 + , and Tween 80, while some metal ions, particularly Fe 3+ , inhibited its activity. The saccharification of several biomass wastes using the crude xylanase enzyme was studied. The results showed the potential for saccharification of alkaline-pretreated wheat bran solution where 75% saccharification was achieved.

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“…Biotechnological processes are widely used to obtain products with high added value [1]. e use of agroindustrial wastes in bioconversion by microorganisms has been the subject of extensive research, especially with reference to the production of metabolites of interest such as proteins, enzymes, organics acids, and secondary metabolites [2]. Cheese whey has been widely used as a fermentation medium, as it is a low-cost cheese residue and is nutritionally rich [3,4].…”

Section: Introductionmentioning

confidence: 99%

Determination of Cell Permeabilization and Beta-Galactosidase Extraction from Aspergillus oryzae CCT 0977 Grown in Cheese Whey

Viana

Pedrinho

Morioka

et al. 2018

International Journal of Chemical Engineering

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Aspergillus oryzae grown in cheese whey has the ability to produce beta-galactosidase. The objective of this work was to define the parameters for the determination of cell permeabilization and extraction of the enzyme from Aspergillus oryzae CCT 0977 biomass, with high enzymatic activity. The Box–Behnken design was used to determine cell permeabilization and extraction of beta-galactosidase conditions. The fermentation was carried out for a period of 5 days at 28°C, having as substrate the deproteinized cheese whey. To determine the effect of the variables on beta-galactosidase activity, enzymatic activity was determined by the lactose hydrolysis reaction. The most efficient condition for cell permeabilization was 25% ethanol at 30°C for 90 min, obtaining an enzymatic activity of 0.44 U·mL−1. For beta-galactosidase extraction from the biomass, the most efficient condition was 5.3% chloroform at 48°C, with an enzymatic activity of 0.17 U·mL−1. The use of ethanol was most efficient to promote cell permeability of Aspergillus oryzae CCT 0977.

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Optimization of xylanase production by Penicillium citrinum xym2 and application in saccharification of agro-residues

Cited by 43 publications

References 26 publications

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

Purification and Characterization of Xylanase from Spent Mushroom Compost and its Application in Saccharification of Biomass Wastes

Determination of Cell Permeabilization and Beta-Galactosidase Extraction from Aspergillus oryzae CCT 0977 Grown in Cheese Whey

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