1992
DOI: 10.1111/j.1432-1033.1992.tb17027.x
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Optimization of yeast‐expressed human liver cytochrome P450 3A4 catalytic activities by coexpressing NADPH‐cytochrome P450 reductase and cytochrome b5

Abstract: Human liver P450 NF25 (CYP3A4) had been previously expressed in Succhuromyces cerevisiue using the inducible GALIO-CYCl promoter and the phosphoglycerate kinase gene terminator [Renaud, J. p., Cullin, C., Pompon, D., Beaune, P. and Mansuy, D. (1990) Eur. J. Biochem. 194,. The use of an improved expression vector [Urban, P., Cullin, C. and Pompon, D. (1990) Biochimie 72,463 -4721 increased the amounts of P450 NF25 produced/culture medium by a factor of five, yielding up to 10 nmol/l. The availability of recent… Show more

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Cited by 82 publications
(46 citation statements)
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“…This activity of P450 NF25 was doubled in the presence of one equivalent of cytochrome b, (data not shown), as previously found for many other activities of this cytochrome (Peyronneau et al, 1992). The two allelic forms of P450 3A4, P450 NF25 and P450 hPCNl, gave similar bromocriptine oxidation activities.…”
Section: Oxidation Of Bromocriptine By Human Liver P450s 3a Expressedsupporting
confidence: 71%
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“…This activity of P450 NF25 was doubled in the presence of one equivalent of cytochrome b, (data not shown), as previously found for many other activities of this cytochrome (Peyronneau et al, 1992). The two allelic forms of P450 3A4, P450 NF25 and P450 hPCNl, gave similar bromocriptine oxidation activities.…”
Section: Oxidation Of Bromocriptine By Human Liver P450s 3a Expressedsupporting
confidence: 71%
“…Expression vector construction, yeast strains, yeast transformation and culture media were previously described (Pompon, 1988;Cullin and Pompon, 1988;Renaud et al, 1990, Peyronneau et al, 1992. Human P450 NF25 cDNA or P450 hPCNl cDNA, which were kindly provided by F. P. Guengerich (Beaune et al, 1986) and F. J.…”
Section: Yeast Strain Yeast Transformation Cell Culture and Microsomentioning
confidence: 99%
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“…An intriguing long standing question has been why is the metabolism of methoxyflurane and a minority of other substrates, such as nifedipine, p-nitroanisole, prostaglandin, lauric acid, n-methylcarbazole, chlorobenzene, p-nitrophenetole, 7-ethoxycoumarin, benzo(a)pyrene, and lidocaine, and testosterone ␤-hydroxylation so stimulated by the presence of cytochrome b 5 while the metabolism of most other substrates, including benzphetamine, is minimally stimulated, not effected, or slightly inhibited in the presence of cytochrome b 5 (Aoyama et al, 1990;Canova-Davis and Waskell, 1984;Hoffman et al, 1989;Okita et al, 1981;Peyronneau et al, 1992;Vatsis et al, 1982).…”
mentioning
confidence: 99%
“…Monkey, mouse, and dog male or female human liver microsomes were obtained from XenoTech LLC, Tebu-Bio (Le Perray en Yvelines, France). Yeast-expressed human P450s were prepared as already described and produced by SPI-BIO (Massy, France) (Peyronneau et al, 1992). The mutated CYP 2C8 isoform R241A expressed in yeast was kindly provided by A. Melet (Unité Mixte Recherche 8601, Centre National de la Recherche Scientifique, Paris, France).…”
mentioning
confidence: 99%