Optimization of α-amylase enzyme activity produced by Bacillus subtilis and Aspergillus niger

Mageed, Baydaa Abd El; Ali, Salah G.; Hammouda, Ola; Raslan, Mai

doi:10.21608/ejchem.2022.136372.6188

Cited by 3 publications

(4 citation statements)

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“…These are some of the typical physical characteristics of crude enzymatic preparations that are used for commercial desizing (http://www.sunsonenzyme.com/Products/ Textile/Desizing/), (https://biosolutions.novozymes.com/en/textiles/ products/desizing/aquazym). The catalytic activity of the enzyme (0.41 U/ml) is similar to that produced by Bacillus subtilis after utilizing different substrates as carbon sources, e.g., treated wheat straw (0.46 U/ml), maize straw (0.47 U/ml), and rice straw (0.44 U/ml) [30], but much lower than those of known commercial desizing amylases [Table 1]. Thus, if Amy LBW 5117 is to be used as a desizing agent, its catalytic activity will have to be enhanced, e.g., through optimizing the culture conditions that were used to grow the bacterium [31].…”

Section: Appearancementioning

confidence: 74%

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Characterization of an alkaline endo-α-1-4-amylase from alkaliphilic Bacillus halodurans LBW 5117 and demonstration of its desizing potential

Oluoch,

Muge,

Mwangi

et al. 2024

J App Biol Biotech

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The demand for amylases with improved storage and operational properties for the economical production of textiles has been on the rise in recent years. An alkaline amylase (Amy LBW 5117) from alkaliphilic Bacillus halodurans LBW 5117 was characterized and used to desize woven cotton. The enzyme had a shelf life of 6 weeks when stored at 4-30°C. It exhibited pH and temperature optima of 10 and 60 o C, respectively. Its activity was stimulated or insignificantly affected by up to 10 mM K + , Ca 2+ , Mg 2+ , Fe 3+ , Na + , and Zn 2+ , as well as Cu 2+ (≤1.0 mM), but was partially inhibited by Mn 2+ (0.5 mM). The enzyme lost all of its activity after 3 h of incubation at 60°C, but this improved to 96% in the presence of 1.0 mM Ca 2+ and 0.05 mM Tween 20. The enzyme was cellulase-free and hydrolyzed starch in an endo-fashion. Furthermore, it degraded and eliminated 8.2% starch size from woven cotton and yielded a fabric that exhibited a TEGEWA rating of 7-8 (residual starch content = 0.0725%) under its optimum operating conditions. This shows that Amy LBW 5117 has good storage and operational properties that potentially make it an effective desizing agent. ARTICLE HIGHLIGHTSAmy LBW 5117 is an alkaline endo-α-1-4-amylase that can desize woven cotton.• At high pH, implying less contamination from neutrophils.• At low temperatures, implying low-energy costs.• In the presence of metal ion impurities, implying savings on the purchase of chelators.

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Section: Appearancementioning

confidence: 74%

“…The effect of temperature on the activity of the enzyme was determined by carrying out the assays at 30,35,40,50,55,60,65, 70, and 90°C using 50 mM glycine-NaOH (pH = 10) under the standard assay conditions.…”

Section: Effect Of Temperature On the Activity Of The Enzymementioning

confidence: 99%

Characterization of an alkaline endo-α-1-4-amylase from alkaliphilic Bacillus halodurans LBW 5117 and demonstration of its desizing potential

Oluoch,

Muge,

Mwangi

et al. 2024

J App Biol Biotech

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show abstract

“…In contrast to prior studies, many utilized raw materials and wastes need an enzymatic pretreatment with amylases, proteases, and lipases to be utilized as commercial fermentation media. Such pretreatments raise the cost of the fermentation process, which contradicts the purpose of waste reuse for cost reduction 20 . The current investigation indicated the ease of utilizing the starch food wastes and used crudely for α-amylase enzyme fermentation without prior pretreatments.…”

Section: Discussionmentioning

confidence: 99%

“…All parameters substantially influenced starch hydrolysis, confirming previous observations on amylase-mediated wastewater remediation 36 . For instance, increased dissolved oxygen was reported after amylase treatment, likely due to organic matter breakdown 23 . In summary, alpha-amylase is a promising, eco-friendly biocatalyst for textile desizing, and wastewater treatment given its specificity and efficiency in starch degradation under suitable conditions.…”

Section: Discussionmentioning

confidence: 99%

Application of Response surface methodology in industrial wastewater treatment using Alpha-amylase from Bacillus amyloliquificiens grown on bread waste

Abd-Elhalim,

Gamal,

El-Sayed

et al. 2023

Preprint

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Food waste is characterized as food loss by consumers or merchants as a result of inadequate storage and a lack of awareness. The objective of this study is to produce alpha-amylase on bread brown waste (BBW) and bread white waste (BWW) using solid medium and submerged fermentation and to use the amylase produced in the treatment of industrial wastewater. B. amyloliquificiens showed the highest starch hydrolysis ratio (SHR) and -amylase activity on both solid medium and submerged fermentation. The production of α-amylase was optimized using the one-factor-at-a-time (OFAT) method. By inoculating BBW (pH 7.0) with 2% (2.3 106 CFU/mL) B. amyloliquificiens and incubating for 24 hours at 37°C and 200 rpm while submerged, the maximum alpha-amylase activity was approached, reaching 695.2 U/mL. The crude enzyme was collected and immobilized on calcium alginate beads. It had an efficiency (IE%) of 96.6% and 88.5% remaining activity after 12 runs of useability. The interaction between the process parameters and the ideal conditions was determined using the Box-Behnken (BBD) statistical design. The quadratic model and one-way analysis of variance (ANOVA) were produced by the BBD to represent the degradation of starch. The experimental values obtained for starch hydrolysis (%) were found to be very close to the predicted response values. According to the design, the ideal conditions were pH 9, 45°C, and 70% and 27.5 U/mL, respectively, of starch after 15 minutes, indicating a high value for the coefficient of determination, R2 (0.83). The alkaliphilic and thermophilic nature of the enzyme activity was confirmed by the ANOVA results, which showed that the quadratic model created from the RSM was statistically significant for starch hydrolysis. At concentrations ranging from 10.9 to 695.1 U/mL, the crude-amylase enzyme was used in the 15-minute desizing process for clothing at 45°C and pH 9.0, and it was discovered to maintain 96.3% of the desizing efficiency. From the study, it could be concluded that the RSM can be a useful tool for optimization and moderation of the process parameters to maximize starch hydrolysis in industrial wastewater treatment.

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Optimizing alpha-amylase from Bacillus amyloliquefaciens on bread waste for effective industrial wastewater treatment and textile desizing through response surface methodology

Abd-Elhalim,

Gamal,

El-Sayed

et al. 2023

Sci Rep

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Food waste is a major issue, with one-third of food wasted yearly. This study aimed to produce sustainably the industrial enzyme alpha-amylase using discarded bread waste. Brown (BBW) and white bread waste (WBW) were tested as growth substrates using solid-state and submerged fermentation. The biosynthesized α- amylase is applied to treat starch-heavy industrial wastewater and for textile desizing. Bacillus amyloliquificiens showed the highest starch hydrolysis and enzyme activity on solid and liquid media. α-amylase production by B. amyloliquificiens was optimized via a one-factor-at-a-time evaluation of production parameters. Optimal production occurred by submerged fermentation of BBW inoculated with 2% B. amyloliquificiens at 37 °C and 200rpm for 24 h, reaching 695.2 U/mL α- amylase. The crude enzyme was immobilized on calcium alginate beads with 96.6% efficiency and kept 88.5% activity after 20 reuses, enhancing stability. A Box–Behnken design (BOX) assessed variable interactions. Response surface methodology (RSM) generated a quadratic model and analysis of variance (ANOVA analysis) fitting experimental starch hydrolysis data. Optimal conditions were pH 9, 45 °C, 70% starch, and 27.5 U/mL enzyme incubated for 15 min of contact time, with a high R2 of 0.83. ANOVA confirmed the enzyme's alkaliphilic and thermophilic nature. Using enzyme concentrations ranging from 10.9 to 695.1 U/mL, the enzyme desized textiles in 15 min at pH 9.0 and 45 °C with 96.3% efficiency. Overall, the optimized α- amylase from bread waste has industrial potential for sustainable starch processing.

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Optimization of α-amylase enzyme activity produced by Bacillus subtilis and Aspergillus niger

Cited by 3 publications

References 0 publications

Characterization of an alkaline endo-α-1-4-amylase from alkaliphilic Bacillus halodurans LBW 5117 and demonstration of its desizing potential

Characterization of an alkaline endo-α-1-4-amylase from alkaliphilic Bacillus halodurans LBW 5117 and demonstration of its desizing potential

Application of Response surface methodology in industrial wastewater treatment using Alpha-amylase from Bacillus amyloliquificiens grown on bread waste

Optimizing alpha-amylase from Bacillus amyloliquefaciens on bread waste for effective industrial wastewater treatment and textile desizing through response surface methodology

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