Optimization Production Conditions of Antibacterial Metabolite From Streptomyces Sp.

Al-Ghazali, Linda H; Omran, Rabab

doi:10.22159/ajpcr.2017.v10i9.19243

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Response surface methodology using OVAT method had been adopted by several workers finding the maximum production of antibacterial compounds fixing the fermentation parameters suitable for the organism [ 65 ]. Antimicrobial activity of the endophytic fungi Aspergillus sp.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity study and metabolic profiling of Colletotrichum alatae LCS1, an endophyte of club moss Lycopodium clavatum L.

Santra

Banerjee

2022

PLoS ONE

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Endophytes are silent microbial partners of green plants that ensure hosts’ survival in odd conditions. They are known as the factories of multipotent metabolites with diverse bioactivities beneficial to modern pharmaceuticals industry. Endophytic fungi have been screened from a variety of plants and it is the first-time endophytes of club moss is being studied for production of antibacterial and antioxidative compounds. The present study reveals that Lycopodium clavatum L. harbors a potent niche of bioactive endophytic fungi and Colletotrichum alatae LCS1 was the prime producer of antibacterial and antioxidative compounds among them. The minimum inhibitory and bactericidal concentrations of ethyl- acetate culture extract ranged from 15.62 to 250 μg/mL against four Gram negative and three Gram positive microorganisms including methicillin resistant Staphylococcus aureus (ATCC-33591). Bio-autogram based screening followed by Gas chromatographic analysis confirmed the occurrence of 17 bioactive compounds and α-bisabolol is known to be the prime one. Alfa bisabolol is a unique and versatile bioactive essential oil and facilitates variety of functions. Killing kinetics data along with leakage of macromolecules into extracellular environment supports the cidal activity of the antibacterial principles at MBC values. Isolate C. alatae LCS1 was optimized by one variable at a time system coupled with response surface methodology for broad spectrum antibacterial production. The organism yielded maximum response (22.66±0.894 mm of zone of inhibition against MRSA) in 250 mL Erlenmeyer flask containing 50 mL potato dextrose broth supplemented with (g/L) glucose, 7.53; yeast extract concentration, 0.47; NaCl, 0.10 with medium pH 6.46; after 134 hours of incubation at 26°C. Optimized fermentation parameters enhanced antibacterial activity up-to more than 50% than the pre-optimized one (10.33±0.57 mm). Endophytic LCS1 was also efficient in free radical scavenging tested by DPPH, ABTS, H2O2 and FRAP assay with an IC50 values of 23.38±5.32 to 82.873±6.479 μg/mL.

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Section: Discussionmentioning

confidence: 99%

Bioactivity study and metabolic profiling of Colletotrichum alatae LCS1, an endophyte of club moss Lycopodium clavatum L.

Santra

Banerjee

2022

PLoS ONE

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“…The crude antibiotics were isolated after 24 h and tested against S. aureus (MTCC 1430). [10][11][12]…”

Section: Methodology Mediamentioning

confidence: 99%

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Sudhan¹

2019

AJP

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Introduction: Five sea cucumber species were collected from fishermen at coastal region of Kanyakumari district, Tamil Nadu, and dissected aseptically. Intestinal fluids and coelomic fluids were collected. After serial dilution of the collected fluids, primary screening was carried out by crowded plate method using modified Soybean Casein Agar media (Kester et al.). Selected antibiotic-producing organisms producing zone of inhibition (ZOI) higher than 6 mm (ZOI >6 mm) were evaluated for broad spectrum of activity using two Gram positive, two Gram negative, and two fungal species by perpendicular streak method. Biochemical characterization and nutrient optimization were carried out for selected three isolates (named IF 32 , IF 52 , and CF 42) which were found to have broad spectrum of activity as well as passed Kirby-Bauer antimicrobial susceptibility test. This article focuses on optimization of temperature, pH, and duration for maximum antibiotic productivity of the selected isolates. Methodology: Optimum temperature, pH, and duration of maximum antibiotic production were evaluated for the selected antibiotic producers. Crude antibiotic was collected after 24 h of incubation under different temperature and pH conditions and tested against Staphylococcus aureus (MTCC 1430). The specific temperature and pH at which maximum ZOI was produced by the collected crude extracts against test organisms were considered. The duration of maximum antibiotic production was evaluated by incubating the isolated organisms under the optimized temperature and pH. Results: The optimum temperature for the production of crude antibiotic from IF 32 , IF 52 , and CF 42 was found to be 32°C, 35°C, and 31°C, respectively. The pH at which maximum antibiotic production was observed is found to be 8, 7, and 9, respectively, for IF 32 , IF 52 , and CF 42. The duration of maximum antibiotic productivity at the optimized conditions was found to be 4 th , 5 th , and 3 rd days, respectively, for IF 32 , IF 52 , and CF 42 .

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A New Trial for Bioformation of Antimicrobial Agent Controlling Multi Drug Resistant Microorganism

Elaasser

2020

Al-Azhar Journal of Pharmaceutical Sciences

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In the present investigation, a trial was done to find a new antimicrobial agent producing microbe from soil microbiota of local habitats to control the problem of multiple drug resistance. The term of Antimicrobial resistance (AMR) is used to describe microorganisms that can resist the effects of drugs and chemicals designed to kill them. Seventy six actinomycetes isolates were isolated from fifteen soil samples different localities in Egypt were primary screening for antimicrobial activity by agar plug diffusion method against test microorganisms. Sixteen isolates were selected for secondary screening in small scale submerged fermentation system and assayed against pathogenic tested microorganisms using agar well diffusion method. Among of these isolates tested, the isolate (S1SHA1) showed the highest antimicrobial activity against pathogenic test organisms. This isolate was identified as Streptomyces griseoplanus by morphological, physiological, biochemical characters and 16s rRNA gene sequence. Physical and nutritional factors affecting activity of antimicrobial agent were studied. The results showed that, optimum activity of antimicrobial agent achieved with pH 7, incubation temperature 28 C, for 7 days, at 150 rpm agitation, carbon and nitrogen source starch 1.5% and potassium nitrate 0.4%, as well as phosphorus 2 g/l and NaCl at concentration of 1 %. Antimicrobial agent from batch culture was subjected to extraction and purification processes using ethyle acetate and preparative TLC, respectively. Determination of minimum inhibitory concentrations (MIC) and Mode of action of antimicrobial agent produced by S. griseoplanus (S1SHA1) on the test microbial strains using Transmission Electron Microscopy (TEM). Cytotoxic studies showed that no cytotoxic effects were observed for the compound when tested even at high concentrations

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Optimization Production Conditions of Antibacterial Metabolite From Streptomyces Sp.

Cited by 4 publications

References 25 publications

Bioactivity study and metabolic profiling of Colletotrichum alatae LCS1, an endophyte of club moss Lycopodium clavatum L.

Bioactivity study and metabolic profiling of Colletotrichum alatae LCS1, an endophyte of club moss Lycopodium clavatum L.

Untitled

A New Trial for Bioformation of Antimicrobial Agent Controlling Multi Drug Resistant Microorganism

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