Optimization studies to develop a low-cost medium for production of the lipases of Rhizopus microsporus by solid-state fermentation and scale-up of the process to a pilot packed-bed bioreactor

Pitol, Luana Oliveira; Finkler, Anelize Terezinha Jung; Dias, Glauco Silva; Machado, Amanda Souza; Zanin, Gisella Maria; Mitchell, David A.; Krieger, Nádia

doi:10.1016/j.procbio.2017.07.019

Cited by 35 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum lipase activity was obtained at 28.19 U/g in the medium containing rice husk, cottonseed cake, and red gram husk combined in the ratio of 2:1:1 under the optimum cultivation conditions of a temperature of 40 • C, a moisture content of 75%, and pH 6.0. Pitol et al [118] were able to achieve a maximum lipase enzyme activity of 264 U/g by adding wheat bran and sugarcane bagasse (50:50 w/w), supplemented only with urea using Rhizopus microsporus CPQBA 312-07 DRM as the enzyme producer. Awan et al [119] used a mixture of almond meal and distilled water as a moistening agent as a substrate with a diluent ratio of 1:0.7, and 0.5% Tween 80 as a medium to produce lipase through the activity of Rhizopus oligosporus ISU-16 in solid-state fermentation.…”

Section: Fibrous Residuesmentioning

confidence: 99%

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

et al. 2021

View full text Add to dashboard Cite

Lipases are enzymes that catalyze various types of reactions and have versatile applications. Additionally, lipases are the most widely used class of enzymes in biotechnology and organic chemistry. Lipases can be produced by a wide range of organisms including animals, plants and microorganisms. Microbial lipases are more stable, they have substrate specificity and a lower production cost as compared to other sources of these enzymes. Although commercially available lipases are widely used as biocatalysts, there are still many challenges concerning the production of microbial lipases with the use of renewable sources as the main component of microbial growth medium such as straw, bran, oil cakes and industrial effluents. Submerged fermentation (SmF) and solid-state fermentation (SSF) are the two important technologies for the production of lipases by microorganisms. Therefore, this review focuses on microbial lipases, especially their function, specificity, types and technology production, including the use of renewable agro-industrial residues and waste materials.

show abstract

Section: Fibrous Residuesmentioning

confidence: 99%

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The main nutrients in the fermentation medium were of carbon (beef extract) and nitrogen (NH 4 NO 3 ) sources. The fermentation biomass depended on the carbon/nitrogen (C/N) ratio and was generally promoted by a higher C/N ratio [34,35]. The experiments showed that Pseudomonas sp.…”

Section: Effects Of Fermentation Conditions On the Growth Of Pseudomomentioning

confidence: 99%

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

Ji-xian

Wang

et al. 2019

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…Perlite was used as inert support (IS) and the optimal water content of perlite medium was 60%. Pitol [42] utilized lowcost medium containing wheat bran and sugarcane bagasse to produce lipase by Rhizopus microspores by SSF and the initial moisture content after inoculation was 65%. Escaramboni [43] reported fast hydrolysis of cassava bagasse sugarcane using R. oligosporus to produce amylase for ethanol biosynthesis, and substrates of agro-industrial waste were prepared with 60% moisture content in SSF.…”

Section: Influence Of Medium Water Content In Enzymatic Production Anmentioning

confidence: 99%

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Tang¹,

Luo²,

Xue³

et al. 2018

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, µ of mycelium reached to 0.15 h − 1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

show abstract

Optimization studies to develop a low-cost medium for production of the lipases of Rhizopus microsporus by solid-state fermentation and scale-up of the process to a pilot packed-bed bioreactor

Cited by 35 publications

References 26 publications

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Contact Info

Product

Resources

About