Optimized conditions for the synthesis of vanillic acid under hypersaline conditions by Halomonas elongata DSM 2581T resting cells

Abstract

During growth on ferulic acid, Halomonas elongata DSM 2581 T was capable of promoting the formation of a significant amount of vanillic acid. The products were confirmed by high-performance liquid chromatography and gas chromatography mass-spectrometry analyses. To enhance the formation of vanillic acid and prevent its degradation, a resting-cell method using Halomonas elongata was developed. The growth state of the culture utilized for biomass production, the concentration of the biomass, the amount of ferulic acid that was treated and the reutilization of the biomass were optimized. The optimal yield of vanillic acid (82%) was obtained after a 10-h reaction using 10 mM ferulic acid and 5 g/l of cell pregrown on ferulic acid and harvested at the end of the exponential phase.

Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid

“…In the first method, fed batch, the cells were harvested in modified SM (1 g/L yeast extract was added as a sole carbon source) and ferulic acid was added when the cells were at the end of the exponential phase. In the second method, resting cell technique (Abdelkafi et al 2008), the cells in mineral medium supplemented with 1 g/L of yeast extract were harvested at the end of the exponential phase by centrifugation, after which cells were washed and suspended in saline/phosphate buffer and then incubated aerobically, at 33°C, in the presence of FA.…”

“…Overhage et al (1999) and Calisti et al (2008) found that ferulic acid is first activated to feruloyl-CoA by a feruloyl-CoA synthetase and then the CoA thioester is subsequently hydrated and cleaved to vanillin and acetylCoA by an enoyl-CoA hydratase/aldolase. Abdelkafi et al (2006Abdelkafi et al ( , 2008 using Halomonas elognata strains found that the ferulic acid was rapidly transformed to vanillic acid at a high yield (80 %). In these studies (Abdelkafi et al 2006(Abdelkafi et al , 2008 however, no vanillin was produced which has a considerably higher value added product than vanillic acid.…”

“…Abdelkafi et al (2006Abdelkafi et al ( , 2008 using Halomonas elognata strains found that the ferulic acid was rapidly transformed to vanillic acid at a high yield (80 %). In these studies (Abdelkafi et al 2006(Abdelkafi et al , 2008 however, no vanillin was produced which has a considerably higher value added product than vanillic acid. Moreover, until now limited microorganisms have been found capable of bioconverting ferulic acid to vanillin at high yield (Table 1).…”

Conversion of Isoeugenol to Vanillin by Psychrobacter sp. Strain CSW4

“…Therefore, the possibility of improving vanillin production from isoeugenol via inhibition of its further degradation and the prevention of biomass duplication by using resting cells strategy has been investigated. Many researchers have successfully explored this strategy for improvement of bioconversion processes [31][32][33]. Maximum vanillin yield (up to 141.45 mg L −1 , molar yield 16.4%) was obtained with this method (Fig.…”

Decarboxylation of Ferulic Acid to 4-Vinyl Guaiacol by Streptomyces setonii

“…Microbial transformations of ferulic acid to a wide variety of aromatic compounds have been reported [7][8][9][10][11][12]. According to Priefert et al [12], the four major pathways of ferulic acid degradation with respect to the initial reaction are (1) nonoxidative decarboxylation, (2) side chain reduction, (3) coenzyme-A-independent deacetylation and (4) coenzyme-Adependent deacetylation.…”