Optimized conversion of wheat straw into single cell oils by Yarrowia lipolytica and Lipomyces tetrasporus and synthesis of advanced biofuels

Caporusso, Antonio; Bari, Isabella De; Liuzzi, Federico; Albergo, Roberto; Valerio, V.; Viola, Egidio; Pietrafesa, Rocchina; Siesto, Gabriella; Capece, Angela

doi:10.1016/j.renene.2022.11.059

Cited by 10 publications

(6 citation statements)

References 75 publications

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“…tetrasporus was able to use both soy flour and urea as N sources. According to our previous results [52], when the yeast was inoculated in the stationary phase of growth as in this condition, it displayed a higher resistance toward thermal degradation by-products in the biomass hydrolysates. Under these conditions, the microorganism first detoxified the hydrolysate, mainly by converting 5-HMF and furfural into less toxic products, [5-(hydroxymethyl)furan-2-yl]methanol and (Furan-2-yl)methanol, respectively.…”

Section: Optimization Of Bench Scale Bioconversion With Three Nitroge...mentioning

confidence: 53%

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Optimization of Wheat Straw Conversion into Microbial Lipids by Lipomyces tetrasporus DSM 70314 from Bench to Pilot Scale

et al. 2023

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Microbial lipids are renewable platforms for several applications including biofuels, green chemicals, and nutraceuticals that can be produced from several residual carbon sources. Lignocellulosic biomasses are abundant raw materials for the production of second-generation sugars with conversion yields depending on the quality of the hydrolysates and the metabolic efficiency of the microorganisms. In the present work, wheat straw pre-treated by steam explosion and enzymatically hydrolysed was converted into microbial lipids by Lipomyces tetrasporus DSM 70314. The preliminary optimization of the enzymatic hydrolysis was performed at the bench scale through the response surface methodology (RSM). The fermentation medium and set-up were optimized in terms of the nitrogen (N) source and carbon-to-nitrogen (C/N) ratio yielding to the selection of soy flour as a N source and C/N ratio of 160. The bench scale settings were scaled-up and further optimized at the 10 L-scale and finally at the 50 L pilot scale bioreactor. Process optimization also included oxygen supply strategies. Under optimized conditions, a lipid concentration of 14.8 gL−1 was achieved corresponding to a 23.1% w/w lipid yield and 67.4% w/w lipid cell content. Oleic acid was the most abundant fatty acid with a percentage of 57%. The overall process mass balance was assessed for the production of biodiesel from wheat straw.

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Section: Optimization Of Bench Scale Bioconversion With Three Nitroge...mentioning

confidence: 53%

“…This is in agreement with the circular economy pursuing zero waste processes. The microbial lipids could be converted into biodiesel, with a yield of 85% [52]. Therefore, starting from 1000 g of WS, it is possible to produce about 98.9 g of biodiesel (about 110.8 mL).…”

Section: Mass Balancementioning

confidence: 99%

Optimization of Wheat Straw Conversion into Microbial Lipids by Lipomyces tetrasporus DSM 70314 from Bench to Pilot Scale

et al. 2023

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“…Biodiesel viscosity is a key physical feature that influences how well it functions as a fuel. Therefore, measuring viscosity is crucial for ensuring product quality [129] . Capillary viscometry, [130] rotational viscometry, [131–133] and optical viscometry [134] are just a few of the methods that can be used to determine the viscosity of biodiesel.…”

Section: Physical Characterization Of Biodieselmentioning

confidence: 99%

“…Therefore, measuring viscosity is crucial for ensuring product quality. [129] Capillary viscometry, [130] rotational viscometry, [131][132][133] and optical viscometry [134] are just a few of the methods that can be used to determine the viscosity of biodiesel. Time taken for a predetermined amount of biodiesel to flow through a capillary tube of defined diameter is used for capillary viscometry.…”

Section: Viscositymentioning

confidence: 99%

A Review on Biodiesel Production, Analysis, and Emission Characteristics from Non‐Edible Feedstocks

Jamil

2023

ChemistrySelect

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Biodiesel produced from different edible and non‐edible feedstocks is a viable alternative option for fueling a combustion engine. However, the widespread use of edible sources for biodiesel production may lead to food versus fuel controversy. Oil from non‐edible feedstock is best possible solution for problems that inhibits biodiesel production on commercial scale. This study presents the potential of different non‐edible feedstock for biodiesel production. Several aspects such as extraction technologies used for extracting oil from non‐edible sources and biodiesel production pathways from non‐edible feedstocks are discussed. The quality of biodiesel should be assured before its commercialization by different physical and chemical characterization techniques are used. In particular, the emission comparison of biodiesel derived from the different non‐edible feedstocks is discussed. Based on this study, the non‐edible feedstocks have the potential to produce biodiesel but still it has a long way to go. This is due to non‐edible feedstock is in the R&D phase and needs more research to eliminate additional treatment steps compared to edible feedstock for economic aspects. Furthermore, there has been a trade off in emission properties of biodiesels produced from non‐edible feedstocks which must be removed or evaluated for pilot scale investigations before commercialization.

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“…Microorganism-derived oils, also known as single-cell oils (SCOs), are similar in composition to vegetable oils [ 17 ] and animal fats [ 11 ]; however, SCOs are preferred over oils derived from plants and animals because it is easy to increase their production.…”

Section: Introductionmentioning

confidence: 99%

Production of Single-Cell Oil from a Local Isolate Bacillus subtilis Using Palm Fronds

Al-Obeidi,

Al-Rawi,

Ali

2023

International Journal of Biomaterials

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This study, conducted at the Department of Biology, University of Anbar, Iraq, focuses on addressing the escalating issue of contamination and aims to acquire microbial oils to alleviate the global shortage in plant and animal oil production, utilizing environmental waste as a carbon source to reduce global pollution and select efficient local bacterial isolates of Bacillus subtilis for the production of single-cell oil (SCO) using local soil and environmental waste as a carbon source. Four isolates were selected as the best in producing single-cell oil, with the isolate with code C4 standing out as it recorded the highest production. It is worth noting that all these isolates belong to the bacteria type Bacillus subtilis. Palm fronds were found to be the most suitable environmental residue for SCO production compared to other waste materials (wheat straw and wheat bran). Submerged cultures were used to improve SCO production, with optimal conditions determined as pH 7, a temperature of 30°C, carbon source concentration of 3 g/100 ml, inoculum volume of 3 ml/100 ml, inoculum density of 20 × 107 cells, and an incubation period of 72 hours. The Soxhlet extraction method was used to obtain the oil, which was found to contain high percentages of unsaturated fatty acids, particularly linoleic acid (46.030%) and palmitoleic acid (16.579%). The oil was highly soluble in chloroform and ethanol but insoluble in water. The saponification test indicated the potential for soap production from the oil. This comprehensive research addresses the need for locally sourced and sustainable SCO production, offering insights into the selection of efficient bacterial isolates, the optimization of cultivation conditions, and the valuable properties of the resulting SCO. The significance of this study lies in the production of single-cell oil from soil-isolated Bacillus subtilis bacteria for use in food applications.

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Optimized conversion of wheat straw into single cell oils by Yarrowia lipolytica and Lipomyces tetrasporus and synthesis of advanced biofuels

Cited by 10 publications

References 75 publications

Optimization of Wheat Straw Conversion into Microbial Lipids by Lipomyces tetrasporus DSM 70314 from Bench to Pilot Scale

Optimization of Wheat Straw Conversion into Microbial Lipids by Lipomyces tetrasporus DSM 70314 from Bench to Pilot Scale

A Review on Biodiesel Production, Analysis, and Emission Characteristics from Non‐Edible Feedstocks

Production of Single-Cell Oil from a Local Isolate Bacillus subtilis Using Palm Fronds

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