To improve the automation level and operation quality of China's beet harvester and reduce the loss due to damaged and missed excavation, this study used a self-developed sugar beet combine harvester and field simulation experiment platform, based on the single-factor bench test of the automatic row following system in the early stage, taking hydraulic flow A, spring preload B, and forward speed C which have significant influence on performance indices as test factors, and taking the missed excavation rate, breakage rate and reaction time as performance indices, the orthogonal experimental study on the parameter optimization of the three-factor and three-level automatic row following system with the first-order interaction of various factors was carried out. The results of the orthogonal experiments were analyzed using range analysis and variance analysis. The results showed that there were differences in the influence degree, factor priority order and first-order interaction, and the optimal parameter combination on each performance index. A weighted comprehensive scoring method was used to optimize and analyze each index. The optimal parameter combination of the overall operating performance of the automatic row following system was A 2 B 2 C 1 , that is, the hydraulic flow was 25 L/min, the forward speed was 0.8 m/s, and the spring preload was 198 N. Under this combination, the response time was 0.496 s, the missed excavation rate was 2.35%, the breakage rate was 3.65%, and the operation quality was relatively good, which can meet the harvest requirements. The comprehensive optimization results were verified by field experiments with different ridge shapes and different planting patterns. The results showed that the mean values of the missed excavation rate of different planting patterns of conventional straight ridges and extremely large "S" ridges were 2.23% and 2.69%, respectively, and the maximum values were 2.39% and 2.98%, respectively; the average damage rates were 3.38% and 4.14%, and the maximum values were 3.58% and 4.48%, which meet the industry standards of sugar beet harvester operation quality. The overall adaptability of the automatic row following system is good. This study can provide a reference for research on automatic row following harvesting systems of sugar beets and other subsoil crop harvesters.