A prompt γ neutron activation analysis (PGNAA) facility based on a 252 Cf spontaneous fission neutron was performed with the final goal of obtaining various optimised radiation protection design parameters. MCNP Monte Carlo code was used to simulate the lead (Pb) shield thickness on two sides of the sample chamber, and B 4 C, B 2 O 3 , LiOH, LiF, and borax were selected as neutron-absorbing materials that were simulated to determine optimal parameters for the optimal design of a biological multilayer composite shield. A series of calculations performed with the MCNP code indicated that the 5 cm thick Pb innermost layer of a biological shield rapidly reduced fast neutrons, and a moderation layer of 25 cm thick paraffin, 20 mm thick borax, and 2 mm thick B 4 C were selected as neutron-absorbing materials. The calculated biological shield protection parameters satisfied the requirements of the PGNAA facility used in the experiment.