“…The AnoxK™5 were seeded carriers harvested from a biological oxygen demand (BOD) removal municipal IFAS wastewater treatment system located in Hawkesbury, Ontario, Canada, and were operated in a single bench lab partial nitritation MBBR system operated at elevated TAN concentrations prior to this study. The reactors were designed at a target TAN SALR of 5 g TAN/ m 2. d, recommended as the optimum for the partial nitritation MBBR system based on the previous findings, as it demonstrated stable and steady performance as well as high partial nitritation rate (Schopf et al, 2019;Ikem et al, 2023). The TAN SALR in the nitrifying MBBR systems at ambient temperature are conventionally designed typically between 0.45 to 1.0 g TAN/m 2. d with respect to target effluent TAN concentrations (Hem et al, 1994;Odegaard, 1999;Young et al, 2017b); thus, the TAN SALR present in this study was significantly elevated (referred to as elevated TAN SALR) than conventional TAN SALR which would potentially allow operating high-rate partial nitritation and small land footprint system.…”