Optimized extraction of neutral carbohydrates, crude lipids and photosynthetic pigments from the wet biomass of the microalga Scenedesmus obliquus BR003

Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus

“…The T. obliquus strain had previously been isolated from freshwater reservoirs and cultivated in BG11 culture media [23,24]. Biomass production was carried out following the protocol established by Vieira et al [17]. The biomass was produced under photoautotrophic growth conditions in a concrete raceway pond of 4 m 3 with a natural photoperiod and light during the summer (Viçosa, Minas Gerais, Brazil).…”

“…The biomass was produced under photoautotrophic growth conditions in a concrete raceway pond of 4 m 3 with a natural photoperiod and light during the summer (Viçosa, Minas Gerais, Brazil). The average temperature in the growing season was approximately 27 • C. The culture media were prepared with macronutrients provided by inorganic fertilizers to increase the protein productivity of T. obliquus through the use of trace elements and without salt stress [13,17,25]. The biomass was collected on the 12th day of cultivation during the stationary growth phase and concentrated using flocculation (Cationic Polyamine, SNF Floerger, Andrézieux, Loire, France) and sedimentation, followed by gravity filtration with a 100 µm polyester membrane, resulting in a microalgal slurry with approximately 5% (w v −1 ) total solids [17].…”

“…The average temperature in the growing season was approximately 27 • C. The culture media were prepared with macronutrients provided by inorganic fertilizers to increase the protein productivity of T. obliquus through the use of trace elements and without salt stress [13,17,25]. The biomass was collected on the 12th day of cultivation during the stationary growth phase and concentrated using flocculation (Cationic Polyamine, SNF Floerger, Andrézieux, Loire, France) and sedimentation, followed by gravity filtration with a 100 µm polyester membrane, resulting in a microalgal slurry with approximately 5% (w v −1 ) total solids [17]. The T. obliquus paste was frozen and stored at −10 • C. The frozen biomass was thawed for fractionation, washed with deionized water three times [26] to remove salts, filtered using an organza fabric filter medium to remove impurities, packed in containers, and frozen (GE, São Paulo, São Paulo, Brazil) at −80 • C. The frozen biomass was freeze-dried (LS 3000, Terroni, São Carlos, São Paulo, Brazil), giving rise to dry biomass, reaching a final moisture content of 8 mass %.…”

“…(i) are native Brazilian species abundant in aquatic environments; (ii) exhibit sizeable morphological variation within each species; (iii) exhibit a high growth rate; and (iv) contain 30% to 40% protein on a dry-mass basis [13,17]. The literature has also described the great potential of T. obliquus biomass for applications in the food, pharmaceutical, cosmetic, and biofuel industries [13,[17][18][19][20][21][22]. Silva et al [2] reported no toxic effects from the ingestion of T. obliquus biomass by Wistar rats.…”

Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

“…On the other hand, another hypothesis claims that the presence of water in the biomass improves nutrient extraction efficiency and can be removed ( Medina et al, 1998 ). Indeed, nutrient extraction such as carbohydrates and lipids from wet microalgal biomass devoid of drying has been fruitful ( Vieira et al, 2021 ). Since the drying step requires at least 89% of energy demand and incurs up to 75% of total processing cost, eliminating this step could prove economical and time-saving for large-scale production of microalgal biomass ( Taher et al, 2014 ).…”