2006
DOI: 10.1159/000092817
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Optimized Growth Kinetics of <i>Pichia pastoris</i> and Recombinant <i>Candida rugosa </i>LIP1 Production by RSM

Abstract: A predictive model for Pichia pastoris expression of highly active recombinant Candida rugosa LIP1 was developed by combining the Gompertz function and response surface methodology (RSM) to evaluate the effect of yeast extract concentration, glucose concentration, temperature, and pH on specific responses. Each of the responses (maximum population densities, specific growth rate (µmax), protein concentration, and minimum lag phase duration) was determined using the modified Gompertz function. RSM and 4-factor-… Show more

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Cited by 13 publications
(8 citation statements)
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“…It was observed that accumulation of the protein in the ER lead to ER stress triggering the unfolded protein response (UPR) pathway. Similar effect of lowered temperature has been demonstrated earlier on production of recombinant LIP1 protein of Candida rugosa [41].…”
Section: Discussionsupporting
confidence: 81%
“…It was observed that accumulation of the protein in the ER lead to ER stress triggering the unfolded protein response (UPR) pathway. Similar effect of lowered temperature has been demonstrated earlier on production of recombinant LIP1 protein of Candida rugosa [41].…”
Section: Discussionsupporting
confidence: 81%
“…One of the most crucial factors influencing proteolysis is known to be the culture pH (Chang et al, ; Daly and Hearn, ; Werten et al, ). As our standard fermentation conditions for C P 4 ‐ type polymers involve pH 3, we now attempted pH 5 for the GS115 strain transformed with T 9 ‐C P 4 ‐D A .…”
Section: Resultsmentioning
confidence: 99%
“…However, each isozyme possesses diVerent substrate speciWcity and thermal stability for biocatalysis, which might lead to divergent catalytic performances [5]. Cloning and expression of the major lipase gene (lip1) is the most acceptable approach for obtaining pure C. rugosa lipase (CRL) with optimized properties for commercial use [3,6,7].…”
Section: Introductionmentioning
confidence: 99%