Optimized hydrodynamic focusing with multiple inlets in MEMS based microfluidic cell sorter for effective bio-cell separation

Kumar, Mahesh; Palekar, Nikhil; Kumar, Ajai; Singh, Kulwant

doi:10.1088/1402-4896/abbaa5

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…The research work presented here is classified into two categories, in the first category, the PDMS material is selected for the cantilever to carry out the analysis of fluid behaviour and cantilever flexibility with the range of Young's modulus 360 kPa-900 kPa. In the second category, materials like eco flex, PDMS, Parylene, Polyimide, Silicon, etc were selected for a cantilever having Young's Modulus in the range of 100 kPa [48,49]. The softer and stiffer microcantilever was placed at the centre of the microchannel and analysed in terms of the maximum deflection.…”

Section: Optimization Flexibility Of T-microcantilever Inside the Mic...mentioning

confidence: 99%

“…The various fields of science and technology including biology and lab on chip devices. Microchannel-based cell separation techniques are widely used in biomedical research and diagnostics [49]. They enable the isolation and enrichment of specific cell populations from complex biological samples, such as blood, tissue, or other bodily fluids [50].…”

Section: Optimization Of Pressure In Microchannel For Rbc and Platele...mentioning

confidence: 99%

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Optimization of Newtonian fluid pressure in microcantilever integrated flexible microfluidic channel for healthcare application

Saxena,

Kumar,

Mishra

et al. 2024

Biomed. Phys. Eng. Express

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The demand for microfluidic pressure sensors is ever-increasing in various industries due to their crucial role in controlling fluid pressure within microchannels. While syringe pump setups have been traditionally used to regulate fluid pressure in microfluidic devices, they often result in larger setups that increase the cost of the device. To address this challenge and miniaturize the syringe pump setup, the researcher introduced integrated T-microcantilever-based microfluidic devices. In these devices, microcantilevers are incorporated, and their deflections correlate with the microchannel's pressure. When the relative pressure of fluid (plasma) changes, the T-microcantilever deflects, and the extent of this deflection provides information on fluid pressure within the microchannel. In this work, finite element method (FEM) based simulation was carried out to investigate the role of material, and geometric parameters of the cantilever, and the fluid viscosity on the pressure sensing capability of the T-microcantilever integrated microfluidic channel. The T-microcantilever achieves a maximum deflection of 127 µm at a 5000 µm/s velocity for Young’s modulus(E) of 360 kPa of PDMS by employing a hinged structure. On the other hand, a minimum deflection of 4.05 x 10-5 µm was attained at 5000 µm/s for Young’s modulus of 1 TPa for silicon. The maximum deflected angle of the T-cantilever is 20.46o for a 360 kPa Young’s modulus while the minimum deflection angle of the T-cantilever is measured at 13.77o for 900 KPa at a fluid velocity of 5000 µm/s. The T-cantilever functions as a built-in microchannel that gauges the fluid pressure within the microchannel. The peak pressure, set at 8.86 Pa on the surface of the cantilever leads to a maximum deflection of 0.096 µm (approximately 1 µm) in the T-cantilever at a 1:1 velocity ratio. An optimized microfluidic device embedded with microchannels can optimize fluid pressure in a microchannel support cell separation.

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Section: Optimization Flexibility Of T-microcantilever Inside the Mic...mentioning

confidence: 99%

Section: Optimization Of Pressure In Microchannel For Rbc and Platele...mentioning

confidence: 99%

Optimization of Newtonian fluid pressure in microcantilever integrated flexible microfluidic channel for healthcare application

Saxena,

Kumar,

Mishra

et al. 2024

Biomed. Phys. Eng. Express

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“…Since DEP does not require cell fixation or labeling, the cells remain viable and are suitable for analysis after sorting [ 12 ]. DEP has been used to manipulate, characterize, and distinguish a wide range of biological particles, such as mammalian cells [ 12 , 13 , 14 , 15 ], bacteria [ 16 , 17 , 18 , 19 , 20 , 21 ], and viruses [ 22 , 23 , 24 ]. Regarding the separation of cancer cells, Gupta et al developed the commercially available Apostream TM to fulfill a need in the marketplace for separating circulating tumor cells from blood without labels [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume

Duncan,

Bloomfield,

Swami

et al. 2023

Micromachines

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Aneuploidy, or an incorrect chromosome number, is ubiquitous among cancers. Whole-genome duplication, resulting in tetraploidy, often occurs during the evolution of aneuploid tumors. Cancers that evolve through a tetraploid intermediate tend to be highly aneuploid and are associated with poor patient prognosis. The identification and enrichment of tetraploid cells from mixed populations is necessary to understand the role these cells play in cancer progression. Dielectrophoresis (DEP), a label-free electrokinetic technique, can distinguish cells based on their intracellular properties when stimulated above 10 MHz, but DEP has not been shown to distinguish tetraploid and/or aneuploid cancer cells from mixed tumor cell populations. Here, we used high-frequency DEP to distinguish cell subpopulations that differ in ploidy and nuclear size under flow conditions. We used impedance analysis to quantify the level of voltage decay at high frequencies and its impact on the DEP force acting on the cell. High-frequency DEP distinguished diploid cells from tetraploid clones due to their size and intracellular composition at frequencies above 40 MHz. Our findings demonstrate that high-frequency DEP can be a useful tool for identifying and distinguishing subpopulations with nuclear differences to determine their roles in disease progression.

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“…In a recent design by Zhang et al, a Y-Y shaped microfluidic chip design where tapered side wall electrodes with opposite polarity were coinciding and hence practically cannot be considered [18]. Kumar et al used three inlets optimized at specific angles for hydrodynamic focusing and achieved good separation of RBCs and yeast cells with separation efficiency above 98% [19]. But any mismatch in velocities of multiple auxiliary inlets may hamper the efficacy of the device.…”

Section: Introductionmentioning

confidence: 99%

“…Enhanced electric field at reduced electrode excitation voltage is the novelty of the proposed microfluidic cell sorter design for avoiding joule heating issues to prevent the damage of biological cells. The proposed device structure is investigated based on standard performance parameters like cell purity, yield and percentage of cell stuck with standard DEP phenomenon [18,19,34,35]. The design proposed in this work can further pave the path to technological development for the separation of various infected tumour cells, bacterial cells, viral cells from healthy blood cells for early-stage health care diagnostics.…”

Section: Introductionmentioning

confidence: 99%

A novel microfluidic device with tapered sidewall electrodes for efficient ternary blood cells (WBCs, RBCs and PLTs) separation

Kumar¹,

Kumar²,

George³

et al. 2021

Meas. Sci. Technol.

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Separation and counting of blood cells from whole blood is very significant in measurement and data extraction in health care diagnostics and monitoring health parameters. Many techniques are available for blood cell separation out of which dielectrophoresis (DEP) on lab on chip platform is an emerging technique due to its phenomenal advantages that the force in this technique acts directly on cells based on dielectric properties. In DEP more challenges lie in optimization of the electric field at lower operating voltages as higher voltages damage the biological cells via joule heating and the separation process cannot be accomplished. Due to this optimization of the electric field distribution in the microchannel of the cell separation device is very much important and can be achieved via numerical model or design optimization of electrodes shapes and material. In this work, a microfluidic cell separation device is optimized using the finite element method for the ternary separation of blood cells. The design is realized with two inlets, three outlets, and one main channel having tapered sidewall electrodes for cell separation purpose. Inlet velocities are optimized to achieve improved cell focusing before subjecting cells to separation effects by varying the velocity ratio of main inlet velocity to buffer inlet velocity. Results have shown successful separation of platelets, red blood cells, and white blood cells with 96.7%–100% yield and purity with velocity ratio from 1:4 to 1:5 at electrode excitation voltage of 1.5 V.

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Optimized hydrodynamic focusing with multiple inlets in MEMS based microfluidic cell sorter for effective bio-cell separation

Cited by 9 publications

References 24 publications

Optimization of Newtonian fluid pressure in microcantilever integrated flexible microfluidic channel for healthcare application

Optimization of Newtonian fluid pressure in microcantilever integrated flexible microfluidic channel for healthcare application

High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume

A novel microfluidic device with tapered sidewall electrodes for efficient ternary blood cells (WBCs, RBCs and PLTs) separation

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