Optimized large-scale optogenetic interface for non-human primates

Griggs, Devon J.; Khateeb, Karam; Philips, Stephen A.; Chan, Jia Wen; Ojemann, William K. S.; Yazdan-Shahmorad, Azadeh

doi:10.1117/12.2511317

Cited by 11 publications

(23 citation statements)

References 20 publications

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“…Furthermore, bringing optical access and electrodes together into a single device would reduce experimental complexity. We previously published designs for a cranial chamber to chronically stabilize the MMAD on the cortex (Griggs et al ., 2019). We expect this stabilization to mitigate variance in day-to-day electrode positioning and reduce perturbations of the cortex which, as previously stated, appeared to accelerate tissue growth on the surface of the brain (Yazdan-Shahmorad et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

“…We expect this stabilization to mitigate variance in day-to-day electrode positioning and reduce perturbations of the cortex which, as previously stated, appeared to accelerate tissue growth on the surface of the brain (Yazdan-Shahmorad et al ., 2016). The cranial chamber is also designed to house the flexible, PCB-free, corrosion-resistant MMAD cables in a coiled configuration between experiments as we have shown bench-side (Griggs et al ., 2019) which will eliminate our previous corrosion issues with permanently mounted PCBs (Yazdan-Shahmorad et al ., 2015).…”

Section: Discussionmentioning

confidence: 99%

“…We have completed bench-side and acute in vivo experiments in this work, and we have previously published designs for a chronically implantable chamber (Griggs et al ., 2019). In the future, we plan to implant the chamber, perform a viral vector transfusion with methods similar to those we have previously published (Yazdan-Shahmorad et al ., 2016; Khateeb et al ., 2019a), and implant the MMAD, molded into its chronic form (Griggs et al ., 2019), in a single surgery. We expect that viral expression will be sufficiently pronounced roughly 2 months after the surgery, at which point we will be prepared to perform chronic in vivo experiments with electrophysiology, electrical stimulation, imaging, and optogenetic modulation.…”

Section: Discussionmentioning

confidence: 99%

“…The angle of the arms was chosen to provide optimal clearance between the clamp-connectors and the sterile field, while still providing room for imaging equipment. The connector holder also included screw posts to aid in affixing or aligning additional experimental equipment to the connector holder, such as optical stimulation devices we have presented previously (Yazdan-Shahmorad et al ., 2015, 2016; Griggs et al ., 2019), although these posts were not used in the experiments presented here.…”

Section: Methodsmentioning

confidence: 99%

“…The MMAD equips us for large-scale acute optical experiments in NHP cortex, such as optogenetic viral vector construct evaluation, with simultaneous electrophysiological recordings. Our MMAD is also designed to bring improved stability to our previously published chronic neural interfaces (Yazdan-Shahmorad et al ., 2015, 2016; Griggs et al ., 2019) which would set the stage for optimized large-scale chronic multi-modal experiments with NHPs in the future. We also outline the remaining steps to chronic MMAD implementation.…”

Section: Introductionmentioning

confidence: 99%

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Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

Griggs

Khateeb

Zhou

et al. 2021

Preprint

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Objective. Non-human primates (NHPs) are critical for development of translational neural technologies because of their neurological and neuroanatomical similarities to humans. Large-scale neural interfaces in NHPs with multiple modalities for stimulation and data collection poise us to unveil network-scale dynamics of both healthy and unhealthy neural systems. We aim to develop a large-scale multi-modal interface for NHPs for the purpose of studying large-scale neural phenomena including neural disease, damage, and recovery. Approach. We present a multi-modal artificial dura (MMAD) composed of flexible conductive traces printed into transparent medical grade polymer. Our MMAD provides simultaneous neurophysiological recordings and optical access to large areas of the cortex (~3 cm2) and is designed to mitigate photo-induced electrical artifacts. The MMAD is the centerpiece of the interfaces we have designed to support electrocorticographic recording and stimulation, cortical imaging, and optogenetic experiments, all at the large-scales afforded by the brains of NHPs. We performed electrical and optical experiments bench-side and in vivo with macaques to validate the utility of our MMAD. Main results. Using our MMAD we present large-scale electrocorticography from sensorimotor cortex of three macaques. Furthermore, we validated surface electrical stimulation in one of our animals. Our bench-side testing showed up to 90% reduction of photo-induced artifacts with our MMAD. The transparency of our MMAD was confirmed both via bench-side testing (87% transmittance) and via in vivo imaging of blood flow from the underlying microvasculature using optical coherence tomography angiography. Significance. Our results indicate that our MMAD supports large-scale electrocorticography, large-scale cortical imaging, and, by extension, large-scale optical stimulation. The MMAD prepares the way for both acute and long-term chronic experiments with complimentary data collection and stimulation modalities. When paired with the complex behaviors and cognitive abilities of NHPs, these assets prepare us to study large-scale neural phenomena including neural disease, damage, and recovery.

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Section: Discussionmentioning

confidence: 99%