Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories

Martín, A.; Herránz, Marta; Lirola, Miguel Martínez; Fernández, Rosa Fernández; Bouza, Emilio; Viedma, Darío García de

doi:10.1186/1471-2180-8-30

Cited by 11 publications

(5 citation statements)

References 14 publications

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“…Positive cultures attributed to laboratory cross‐contamination were reported to contribute up to 3% of culture‐positive cases . Changing from the relatively slow RFLP typing to the much faster VNTR typing should help in the early identification of laboratory cross contamination . This is important, as laboratory cross‐contamination may lead to false or delayed diagnosis and unnecessary health risks and costs associated with treatment and hospital admission .…”

Section: Molecular Epidemiological Insights From the Last Two Decadesmentioning

confidence: 99%

The re-emergence of tuberculosis: what have we learnt from molecular epidemiology?

Borgdorff

Soolingen

2013

Clinical Microbiology and Infection

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Tuberculosis (TB) has re-emerged over the past two decades: in industrialized countries in association with immigration, and in Africa owing to the human immunodeficiency virus epidemic. Drug-resistant TB is a major threat worldwide. The variable and uncertain impact of TB control necessitates not only better tools (diagnostics, drugs, and vaccines), but also better insights into the natural history and epidemiology of TB. Molecular epidemiological studies over the last two decades have contributed to such insights by answering long-standing questions, such as the proportion of cases attributable to recent transmission, risk factors for recent transmission, the occurrence of multiple Mycobacterium tuberculosis infection, and the proportion of recurrent TB cases attributable to re-infection. M. tuberculosis lineages have been identified and shown to be associated with geographical origin. The Beijing genotype is strongly associated with multidrug resistance, and may have escaped from bacille Calmette-Guérin-induced immunity. DNA fingerprinting has quantified the importance of institutional transmission and laboratory cross-contamination, and has helped to focus contact investigations. Questions to be answered in the near future with whole genome sequencing include identification of chains of transmission within clusters of patients, more precise quantification of mixed infection, and transmission probabilities and rates of progression from infection to disease of various M. tuberculosis lineages, as well as possible variations in vaccine efficacy by lineage. Perhaps most importantly, dynamics in the population structure of M. tuberculosis in response to control measures in high-prevalence areas should be better understood.

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Section: Molecular Epidemiological Insights From the Last Two Decadesmentioning

confidence: 99%

The re-emergence of tuberculosis: what have we learnt from molecular epidemiology?

Borgdorff

Soolingen

2013

Clinical Microbiology and Infection

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“…The MST method, which was used in this study in addition to the more commonly used VNTR/MIRU typing method [ 15 , 16 ], requires a relatively small amount of sample DNA from the patient. In contrast to the conventional IS 6110 -RFLP method, which requires a relatively large amount of DNA, both the MST and the VNTR/MIRU typing methods require only small DNA samples as they are based on PCR amplification of selected genomic regions [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…A similar, yet slightly longer delay of 13 days (median value) between initial analysis and interpretation of results was recently reported when using the VNTR/MIRU method. In contrast, the conventional IS 6110 technique provided results in a median time of 45 days [ 16 ]. The delay period required to complete the MST analysis is certainly short enough to contribute to the interpretation of laboratory data that may have a significant clinical impact on patients.…”

Section: Resultsmentioning

confidence: 99%

“…More importantly from, a strictly technical perspective, IS 6110 -RFLP analysis does a poor job of indicating the presence of M. tuberculosis when these organisms contain only a few copies of the IS 6110 sequence [ 13 ]. Recently, the variable number tandem repeat (VNTR) PCR-based technique and the mycobacterial interspersed repetitive unit (MIRU) [ 14 ] technique have proven to be reliable methods for the resolution of cross-contamination events [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection of laboratory cross-contamination with Mycobacterium tuberculosis using multispacer sequence typing

2009

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BackgroundThe ability to culture Mycobacterium tuberculosis from clinical specimens serves as the gold standard for the diagnosis of tuberculosis. However, a number of false-positive diagnoses may be due to cross-contamination of such specimens. We herein investigate such episode of cross-contamination by using a technique known as multispacer sequence typing (MST). This technique was applied to six M. tuberculosis isolates prepared within the same laboratory over a two-week period of time.ResultsMST analysis indicated a unique and common sequence profile between a strain isolated from a patient with proven pulmonary tuberculosis and a strain isolated from a patient diagnosed with lung carcinoma. Using this approach, we were able to provide a clear demonstration of laboratory cross-contamination within just four working days. Further epidemiological investigations revealed that the two isolates were processed for culture on the same day.ConclusionThe application of MST has been demonstrated to serve as a rapid and efficient method to investigate cases of possible cross-contamination with M. tuberculosis.

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“…In addition, it increased the epidemiological knowledge of M. bovis population and structure, evolutionary steps which is advantageous for the study of TB outbreaks and for understanding the dynamics of the disease in the area [57][58][59][60][61]. This will help in the control of TB strains distribution and treatment regimen by indicating the active transmission and reactivation of a Mycobacterium strain.…”

Section: Mycobacterium Bovis Prevalence Studiesmentioning

confidence: 99%

Molecular Characterization of Mycobacterium bovis and its Significance: Role for Control of Zoonotic Tuberculosis in Africa

Getahun¹

2017

J Med Diagn Meth

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Mycobacterium bovis is the main causal agent of bovine tuberculosis (BTB) that causes zoonotic tuberculosis (TB) in humans, even though M. caprae; contribute to a lesser extent, which is mostly acquired from domestic animals and their products, in which cattle are the major reservoir. This review paper discussed the importance of zoonotic tuberculosis in Africa and highlighted the uses of molecular methods in diagnosis, control and prevention of zoonotic tuberculosis. Despite the fact, there are various diagnostic methods available nowadays; the molecular techniques are proving more rapid and plausible sensitivity and specificity results that increase the diagnosis precision. In addition, molecular diagnostic methods used to indicate the epidemiological status of different strain distribution with regard to cluster distribution and reactivation of dormant or treated cases of tuberculosis indicating the drug resistance situation of the strain. Hence, applying molecular techniques for diagnosis of zoonotic tuberculosis in developing countries will help in decreasing the incidence, prevalence, mortality, morbidity and loss of economic due to carcass condemnation, milk drop and livestock trade banning in the continent. Advanced molecular methods truly play a key role in prevention and control program of tuberculosis in general and bovine tuberculosis in specific, which leads to the cooperation of various disciplines. Introducing cost effective and easy molecular diagnostic methods is recommended, implementing molecular diagnostic methods in research areas and intensive training of personnel in the use of equipment.

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Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories

Cited by 11 publications

References 14 publications

The re-emergence of tuberculosis: what have we learnt from molecular epidemiology?

The re-emergence of tuberculosis: what have we learnt from molecular epidemiology?

Rapid detection of laboratory cross-contamination with Mycobacterium tuberculosis using multispacer sequence typing

Molecular Characterization of Mycobacterium bovis and its Significance: Role for Control of Zoonotic Tuberculosis in Africa

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