The examination of alterations in fundamental soil properties as a consequence of longterm agricultural usage holds significant theoretical and practical implications. This research was primarily designed to analyze the agroecological transformation of zonal soil fertility attributes due to extended agricultural practices in Northern Kazakhstan. The methodological approach adopted in this study incorporated soil and landscape mapping, supplemented by Geographic Information System (GIS) interpolation techniques. The focus of the paper is on the temporal evolution of key soil components -namely humus, nitrogen, mobile phosphorus, and exchangeable potassium -over an extended period of agricultural land use within the Kostanay district, situated in the Kostanay region. The Soil Absorbing Complex (SAC) of the scrutinized soils was found to be predominantly composed of calcium (77-87%) and magnesium (11-15%). Over the course of a 33-year timeline, a slight reduction in the levels of humus, nitrogen, phosphorus, and potassium was observed. It was discerned that prolonged agricultural practices instigated minor decremental trends in crucial soil fertility parameters. In essence, the study offers valuable insights into the subtle, yet significant, impacts of long-term agricultural land use on soil fertility, contributing to our understanding of sustainable agricultural practices. This research underscores the need for careful management of agricultural lands to preserve soil health and ensure long-term productivity.