2010
DOI: 10.1002/ijc.25364
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Optimized nanosecond pulsed electric field therapy can cause murine malignant melanomas to self‐destruct with a single treatment

Abstract: We have identified a new, nanosecond pulsed electric field (nsPEF) therapy capable of eliminating murine melanomas located in the skin with a single treatment. When these optimized parameters are used, nsPEFs initiate apoptosis without hyperthermia. We have developed new suction electrodes that are compatible with human skin and have applied them to a xenograft nude mouse melanoma model system to identify the optimal field strength, pulse frequency and pulse number for the treatment of murine melanomas. A sing… Show more

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Cited by 130 publications
(97 citation statements)
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“…They developed different protocols and eventually obtained the destruction of all of the 17 treated nodules in 4 mice with only one sequence of 100 ns pulses [Nuccitelli et al, 2010]. In their experiments, which included more than 300 tumors on 159 mice, they observed tumor cells with nuclear pyknosis and reduced blood flow, as well as red blood cell leakage within a few minutes after treatment [Nuccitelli et al, 2006.…”
Section: In Vivo Studies Using Nanopulsesmentioning
confidence: 98%
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“…They developed different protocols and eventually obtained the destruction of all of the 17 treated nodules in 4 mice with only one sequence of 100 ns pulses [Nuccitelli et al, 2010]. In their experiments, which included more than 300 tumors on 159 mice, they observed tumor cells with nuclear pyknosis and reduced blood flow, as well as red blood cell leakage within a few minutes after treatment [Nuccitelli et al, 2006.…”
Section: In Vivo Studies Using Nanopulsesmentioning
confidence: 98%
“…The tumor response appeared dependent on the amplitude and the number of pulses along with the electrode configuration. Even though apoptosis appears to be the primary mechanism inducing cell destruction because the vascularization of the tissue is disrupted, necrosis is very likely to happen [Nuccitelli et al, 2006[Nuccitelli et al, , 2010.…”
Section: In Vivo Studies Using Nanopulsesmentioning
confidence: 98%
“…If all of the stored energy were released within one microsecond, the power would be one megawatt, a million times greater and if release in one nanosecond the peak power would be one gigawatt, a billion times greater. Within the last ten years, applications of ultra-high pulse power have been extended to biology and medicine [Beebe et al, 2002[Beebe et al, , 2003aBeebe and Schoenbach, 2005;Schoenbach et al, 2001Schoenbach et al, , 2004Vernier et al, 2003a,b], including the treatment of melanoma Ford et al, 2010;Nuccitelli et al, 2006Nuccitelli et al, , 2009Nuccitelli et al, , 2010. In applications of pulse power ablation for treatment of melanoma, as much as 6 kV of potential energy were release in multiple 300ns bursts at 60kV/cm into murine B16f10 melanoma cells or tumors.…”
Section: Applications Of Ultra-high Pulse Power Ablation For the Treamentioning
confidence: 99%
“…These studies used the first developed electrode design for in vivo applications of pulse power to tumors. Since then significant advances with a number of electrode designs have been used Garon et al, 2007;Nuccitelli et al, 2006Nuccitelli et al, , 2010. The fibrosarcoma studies followed studies showing that pulse power with nanosecond pulsed electric fields induced markers for apoptosis in Jurkat and HL-60 cells [Beebe et al, 2002].…”
Section: Multiple Mechanisms For Nspefs To Eliminate Tumors In Vivomentioning
confidence: 99%
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