2022
DOI: 10.18502/pbr.v8i2.11029
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Optimized Primary Culture and Subculture of Granulosa Cells

Abstract: Background: Primordial follicle includes an oocyte surrounded by a layer of somatic cells called Granulosa Cells (GCs). GCs, also known as nurse cells, are an important protective element for the growth and survival of oocytes. Oocytes, which lack some of the metabolic processes, require granulosa cells for their development. Objectives: This manuscript was provided to explain the protocol of GCs primary culture extracted from NMRI mice ovaries. Methods: For choosing the optimum protocol, we used t… Show more

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